Résumé
Objective To rapidly identify the chemical components in a traditional Chinese compound herbal preparation Babao Dan by ultra-high performance liquid chromatography-quadrupole-time of flightmass spectrometry (UHPLC-Q-TOF/ MS). Methods A formula database including 547 compounds was developed by Agilent software " Formula-Database Generator”. The total ion chromatograms of the Baboo Dan extracts were obtained by UHPLC-Q-TOF/MS, and the chemical components were identified by automatic matching method according to the exact mass-to-charge ratio of each chemical component The chemical components of Baboo Dan was further verified using the secondary fragment ion obtained by adjusting the secondary monitor voltage of Q-TOF/MS. The separation was performed on a Waters Xbridge BEH C18 column (2.1 mm X 100 mm, 2.5 μm). The mobile phase composing of acetonitrile (A) and 0.5% formic acid aqueous solution (B) was used for gradient eluting (0-5 min, 5% A; 5-25 min, 5%-95% A; 25-30 min, 95% A). The temperature of column was 25°C; the flowing rate was 0.8 mL/min; and the post-column spit ratio was 2:1. The Q-TOF/MS and electro spray ion source (ESI) were applied for qualitative analysis under positive and negative ion mode, with the mass scan range being m/z 100-1 500. The capillary voltage was 3 500 V under positive ion mode and 4 000 V under negative ion mode, and the range of collision energy of MS2 was 10-40 eV. Results UHPLC-Q-TOF-MS identified a total of 78 chemical components from Babao Dnn, including saponins, bile acids, amino acids and so on. Conclusion A rapid and efficient method for identifying the chemicaf components of Babao Dan by UHPLC-Q-TOF-MS has been established, and the chemical components of Babao Dan has been clarified, which lays a foundation for the quality control and further pharmacological study of Babao Dan.
Résumé
Objective: To study the chemical components in the root of Asparagus cochinchinensis (Lour.) Merr. Methods: Chemical components of Asparagus cochinchinensis root were isolated by column chromatography on macroporous resin, Silgel, Sephadex LH-20, ODS, etc. and the structures of the components were identified by physiochemical and spectral analysis, such as MS, 1HNMR, and 13 CNMR. Results: The following 5 components were isolated and identified: diosgenin-3-O-p-β-glu- copyranoside(I), smilagenin(II), 26-O-β-D-glucopyranosyl-furost-3β, 22, 26-triol-3-0-p-D-glucopyranosyl(1→2)-O-β-D-glu- copyranoside(JU) 26-0-β-D-glucopyranosyl-furost-5, 20-en-3β, 2α, 26-triol-3-O-[α-α-rhamnopyranosyl (1[2)]-[α-α-rhamnopyranosyl-(1→4)]-β-D- glucopyranoside(IV), and 26-O-β-D-glucopyranosyl-furost-3β, 26-diol-22-methoxy-3-0-α-L-rhamnopyranosy (1-4)-O-β-D-glucopyranoside (V). Conclusion: All the 5 compounds isolated from Asparagus cochinchinensis in this study is reported for the first time.
Résumé
Objective: To make a comparative study on the root and stalk of Actinidia valvata, so as to lay a ground for expanding medicinal part of Actinidia valvata. Methods: A comparative study was made between the root and stalk of Actinidia valvata on the following aspects: macroscopical properties, physical and chemical properties, contents of active constituents, and pharmacological actions. Results: The root and stalk of Actinidia valvata both showed positive reaction for alkaloids and saponin, and both had 280 nm absorption peak in UV-Vis spectrum. The aqueous extraction yield of the root and stalk were 5.35% and 5.68%, respectively; the contents of dihydrodehydrodiconiferyl alcohol were 0.005 01% and 0.006 41%, respectively. The IC50 of root and stalk extracts against HL60 were 62.39 mg and 75.51 mg, respectively; and against K562 were 70.47 mg and 77.46 mg, respectively. Their extracts had similar scavenging activity for free radicals and reductive effect; neither of them had analgesia actions. Conclusion: The root and stalk of Actinidia valvata have similar physical and chemical properties, contents of active constituents and pharmacological actions, suggesting that it is highly possible to substitute the root of Actinidia valvata with stalk for medicinal usage.
Résumé
Objective:To study the chemical components in the root of Asparagus cochinchinensis(Lour.)Merr.Methods:Chemical components of Asparagus cochinchinensis root were isolated by column chromatography on macroporous resin,Sil-gel,Sephadex LH-20,ODS,etc.and the structures of the components were identified by physiochemical and spectral analysis,such as MS,1HNMR,and 13CNMR.Results:The following 5 components were isolated and identified:diosgenin-3-O-?-D-glucopyranoside(Ⅰ),smilagenin(Ⅱ),26-O-?-D-glucopyranosyl-furost-3?,22,26-triol-3-O-?-D-glucopyranosyl(1→2)-O-?-D-glucopyranoside(Ⅲ),26-O-?-D-glucopyranosyl-furost-5,20-en-3?,2?,26-triol-3-O-\[?-?-rhamnopyranosyl(1→2)\]-\[?-?-rhamnopyranosyl-(1→4)\]-?-D-glucopyranoside(Ⅳ),and 26-O-?-D-glucopyranosyl-furost-3?,26-diol-22-methoxy-3-O-?-L-rhamnopyranosy(1→4)-O-?-D-glucopyranoside(Ⅴ).Conclusion:All the 5 compounds isolated from Asparagus cochinchinensis in this study is reported for the first time.