Résumé
Objective To observe the protective effect and mechanism of taraxasterol on cardiomyocytes in oxidative injury model caused by ischemia-reperfusion (I/R).Methods Mouse cardiomyocytes (CSC cells) were used as the study objects and divided into the normal control group,I/R group,taraxasterol treating I/R group (5,10,30 μmol/L) and positive control group.The cell viability was measured by MTT.The expressions of Caspase-3 and Bcl-2 were detected by RT-PCR.The expressions of superoxide dismutase (SOD) and malondialdehyde (MAD) were detected by biochemical methods.The expression of ERK1/2 was detected by western blot.Results MTT assay showed that 30 μmol/L taraxasterol increased the cell viability of CSC cells injured by I/R (P＜0.05).The RT-PCR results showed that the expression of Caspase-3 mRNA was decreased with 10,30 μmol/L taraxasterol treatment,the difference was statistically significant when compared with I/R group (P＜0.05).The expression of Bcl-2 mRNA was increased with 30 μmol/L taraxasterol treatment,the difference was statistically significant when compared with the I/R group (P＜0.05).The biochemical method detection showed that 30 μmol/L taraxasterol induced SOD expression was increased and MAD expression was decreased,the difference was statistically significant when compared with the I/R group (P＜0.05).Western blot detection showed that 30 μmol/L taraxasterol treatment increased the ratio of p-ERK1/2 to t-ERK1/2 in injured CSC cells (P＜0.05).Conclusion Taraxasterol might inhibit ischemia-reperfusion caused cardiomyocyte oxidative injury by up-regulation of ERK1/2 expression.
Résumé
Objective: To isolate and identify the chemical constituents of petroleum ether soluble part of 60% aq. ethanol extracts from the whole herb of Mulgedium tataricum. Methods: Separation and purification were performed on silica gel column chromatography and recrystallization, PTLC, PHPLC, and related techniques. Their chemical structures were elucidated through spectroscopic analyses (NMR). Results: Nine compounds were isolated and identified as taraxasterol (1), pseudotaraxasterol (2), lupeol (3), olean-18-en-3β-ol (4), β-sitosterol (5), olean-18-en-3-one (6), 3β-hydroxy-taraxaster-20(30)-ene-28-oic acid (7), stigmasterol (8), and lupenone (9), respectively. Conclusion: Compounds 3, 4, 6, 7, and 9 are separated from this plant for the first time.
Résumé
OBJECTIVE: To study the chemical constituents in Ainsliaea bonatii. METHODS: The column chromatographic techniques were used to isolate the constituents in A. bonatii. EI-MS and NMR were used to identify the constituents structures. RESULTS: Seven compounds were isolated and identified from the extract of A. bonatii, whose structures were elucidated as taraxasterol (1), β-sitosterol (2), nodakenetin (3), 1, 4-benzenediol (4), daphnetin (5), apigenin (6) and β-arbutin (7). CONCLUSION: Compounds 1-5 and 7were isolated for the first time from this plant, and 1, 3-5 were isolated for the first time from Ainsliaea genus.