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AIM: To study protective effects of the extract from Toona sinensis seeds on gastric mucosal injury in experimental mice. METHODS: The mice were given 200 mg/kg, 400 mg/kg of Toona sinensis seeds every day for 2 weeks and ethanol or aspirin was used to induce gastric mucosa injury model. The gastric mucosal injury index and injury inhibition rate were calculated, the levels of SOD, MDA in serum and the contents of ET-1, PGE
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Objective To investigate the inhibition of aldose reductase (AR) and antioxidant activity of fractions of ethyl acetate extract from Toona Sinensis seeds. Methods The antioxidant activities of fractions of ethyl acetate extract from Toona Sinensis seeds was evaluated with total antioxidant capacity and DPPH?free radicals scavenging. The inhibition and inhibition types of AR were investigated and the relationship of antioxidant activity and AR inhibitory activity was analyzed. Results Fractions of ethyl acetate extract from Toona Sinensis seeds exhibited antioxidant activity, total antioxidant capacity ordered by Fr.B>Fr.A>Fr.D>Fr.C. The DPPH?free radicals scavenging were ordered by Fr.A>Fr.B>Fr.D>Fr.C. The fractions exhibited AR inhibitory activity and the order was Fr.B>Fr.A>Fr.C>Fr.D. The inhibition mechanism of Fr.B was noncompetitive inhibition and IC50 was 0.401 mg/ml. The AR inhibitory activity of fractions was related to antioxidant activity with correlation index over 0.9. Conclusion This study will provide theoretical basis for exploitation of Toona Sinensis seeds.
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Objective To investigate the protective effects of Toona sinensis leaf extract on the retina of rats with high-fat diet and the expression of B-cell lymphoma (Bcl-2) and Bcl-2 associated x protein (Bax).Methods Together 24 male SD rats were randomly divided into normal group (N group),hyperlipidemia model group (HF group) and hyperlipidemia model + toona sinensis leaf extract (HF + TSLE group),and then hyperlipidemia model was induced by fed high-fat diet in the latter two groups;after 8 weeks,the model was confirmed to be successful,and the rats in HF + TSLE group were fed with TSLE solution for 4 weeks continuously,and rats in N group and HF group were given the same dose of physiological saline.At the end of the twelfth week,all rats were followed by the examination of flash electroretinogram (FERG),serum lipid total cholesterol (TC),triglyceride (TG),low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C).Then,HE staining was performed in the retinas,the expression of Bcl-2 and Bax was detected by immunohistochemistry and Western blotting for the analysis of the correlation between the expression level of apoptotic protein Bax and the abnormal function of FERG.Results In HF group,the content of HDL-C decreased,and the contents of TC,TG and LDL-C were higher than those in N group,and the differences were statistically significant (all P ＜ 0.05).The contents of TC,TG and LDL-C in HF + TSLE group were lower than those in HF group,but the content of HDL-C was significantly increased,and the differences were statistically significant (all P ＜ 0.05).The content of HDL-C in HF + TSLE group was lower than that in N group,while the contents of TC,TG and LDL-C were higher than those in N group,and the differences were not statistically significant (all P ＞0.05).The difference of a wave latency between the three groups was statistically significant (P ＜ 0.05),and the latency of a wave in HF group was longer than that in N group,while HF + TSLE group was shorter than HF group,and the difference was statistically significant (P ＜ 0.05),but HF + TSLE group was longer than N group,and the difference was not statistically significant (P ＞ 0.05).Moreover,there was no significant difference in the incubation period of b wave in the three groups (P ＞ 0.05);and there was no significant difference in the amplitude of a wave and b wave in the three groups (all P ＞ 0.05).In addition,HF group had lower expression level of Bcl-2 and overexpression of Bax than N group.The expression level of Bcl-2 increased and Bax expression level decreased significantly in HF + TSLE group,and the expression level of Bax was positively correlated with the latency of a wave and b wave (all P ＜ 0.05),but was not correlated with amplitude of a wave and b wave (all P ＞ 0.05).Conclusion TSLE has an important role in the retina of rats with abnormal lipid metabolism,and it may play a protective role by regulating the expression of Bcl-2 and Bax.
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AIM To establish a UPLC method for the simultaneous content determination of glycosides,rutinoside,myricitrin,hyperoside,isoquercitrin,guaijaverin,astragalin,quercitrin and afzelin in the shoots of Toona sinensis Roemer.METHODS The analysis of 60％ methanol extract of T.sinensis shoots was performed on a 35 ℃ thermostatic Waters XBridge Shield RP18 column (2.1 mm × 150 mm,1.7 μm),with the mobile phase comprising of acetonitrile-water flowing at 0.35 mL/min in a gradient elution manner,and the detection wavelength was set at 350 nm.RESULTS Eight flavonol glycosides showed good linear relationships within their own ranges (r≥ 0.9992),whose average recoveries were 98.3％-103.5％ with the RSDs of 0.46％-3.36％.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of T.sinensis shoots.
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AIM To establish a UPLC method for the simultaneous content determination of glycosides,rutinoside,myricitrin,hyperoside,isoquercitrin,guaijaverin,astragalin,quercitrin and afzelin in the shoots of Toona sinensis Roemer.METHODS The analysis of 60％ methanol extract of T.sinensis shoots was performed on a 35 ℃ thermostatic Waters XBridge Shield RP18 column (2.1 mm × 150 mm,1.7 μm),with the mobile phase comprising of acetonitrile-water flowing at 0.35 mL/min in a gradient elution manner,and the detection wavelength was set at 350 nm.RESULTS Eight flavonol glycosides showed good linear relationships within their own ranges (r≥ 0.9992),whose average recoveries were 98.3％-103.5％ with the RSDs of 0.46％-3.36％.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of T.sinensis shoots.
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Objective To optimize the conditions of extraction process of polysaccharides from seeds of Toona sinensis; To preliminary determinate the antioxidant activity in vitro. Methods The content of polysaccharides from Toona sinensis was chosen as evaluation index; the extraction time, material liquid ratio, and extraction times were chosen as factors; L9(34) orthogonal design was used to optimize extraction process; antioxidant activity of polysaccharides from Toona sinensis was investigated by the total reducing power and 1,1-diphenyl-2-picryhydrazyl (DPPH) method. Results From the statistical data of processing, the optimal extracting conditions were as follows:liquid to material was 1:12, extraction time was 120 min, and extracted for 3 times. Polysaccharides from seeds of Toona sinensis had certain reducing capacity and had better scavenging ability on DPPH? and the scavenging rate significantly increased with the concentration. The effect was simulated by curve equation. Conclusion The process is simple, feasible, stable and reliable and can be used for the small-scale study on extraction of polysaccharides from seeds of Toona sinensis. Polysaccharides from seeds of Toona sinensis have antioxidant activity.
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OBJECTIVE: To study the chemical constituents in the leaves of Toona sinensis Roemor. METHODS: Silica gel column chromatography and preparative high performance liquid chromatography were used to isolate constituents from dichloromethane and ethyl acetate portion of ethanol extract of this Chinese medicine. Subsequently, the chemical structures were elucidated by NMR spectral data and physical and chemical properties. RESULTS: Eight compounds are isolated and elucidated as scopoletin(I), 4, 7-dimethoxy-5-methylcoumarin (II), (+)-catechin(III), quercetin-3-O-α-L-rhamopyranoside(IV), kaempferol-3-O-α-L-rhamopyrano-side(V), l, 2, 3, 4, 6-penta-O-galloyl-β-D-glucopyranose(VI), astragalin (VII) and ethyl gallate(VIII). CONCLUSION: Except for compounds IV, VI-VIII, the other four compounds are isolated from Toona sinensis Roemor for the first time, and compound V is for the first time isolated from Toona genus. Copyright 2013 by the Chinese Pharmaceutical Association.
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Objective:To determine the antioxidization and antiproliferation of extract from leaves of Toona sinensis (LTS).Methods:The total phenolic extract of LTS was obtained by solvent and polyamide resin to determine the content.The antioxidization of the LTS extract was measured by TOSC assay.Antiproliferation was studied in vitro with different human cancer cells.Results:The total phenolic content in the LTS was (427.53±4.31) mg/g and antioxidization was 807.64 μmoL vitamin C equivalents/g in the sample.The extract significantly inhibited the colon cancer cell Caco-2,human liver cancer cell HepG2 and breast cancer cell MCF-7 proliferation with EC50 (4.00±0.39),(153.16±13.49) and (193.46±14.68) μg/mL,respectively.The bioactivity index (BI) of the LTS extract was nearly 283.Caco-2 was more sensitive than MCF-7 and HepG2.Conclusion:Extract from LTS has anticancer properties useful for preventing chronic diseases.
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Object To proceed our continual search of insecticides and potentially useful pharmaceutics in plants of the Meliaceae family Methods Chemical constituents in the ethanolic extract of the leaves of Toona sinensis (A Juss ) Roem were separated and identified Results 6 compounds were isolated and identified as: 6, 7, 8, 2′ tetramethoxy 5, 6′ dihydroxy flavone (Ⅰ); 5, 7 dihydroxy 8 methoxy flavone (Ⅱ); kaempferol (Ⅲ); 3 hydroxy 5, 6 epoxy 7 megastigmen 9 one (Ⅳ), ethyl gallate (Ⅴ) and scopoletin (Ⅵ) by spectral methods Conclusion Compound Ⅳ was obtained from Toona Roem. for the first time