Résumé
OBJECTIVE:To study the analgesic effect of total flavonoids of propolis(TFP)and its mechanism.METH?ODS:The analgesic effect test is performed by formaldehyde method,hot-plate method,warm bath method and writhing method.The mice were randomly divided into normal saline(NS)control group,morphine positive control group and different dosage of TFP group,each pain index under each experiment condition was determined after intragastric administration of each group of mice.RESULTS:Compared with the NS control group,TFP group showed a decreased scoring in pain reaction(15min later),reduced writhing response(50,100mg/kg),prolonged latent phases of foot licking(50,100mg/kg)and tail-shrinking(50mg/kg),significant decrease of the content of malonaldehyde,prostaglandin-2and NO in mice'blood serum and brain tissue.CONCLUSION:TFP have notable analgesic effect,the mechanism may be related to its function on inhibiting prostaglandin-2and lipid peroxidation and reducing the release of NO releasing of brain tissue.