Résumé
OBJECTIVE: To investigate the inhibition effect of total alkaloid, total phenolic acid and total volatile oil from Ligusticum chuanxiong on the contraction of the isolated uterine smooth muscle of rats, and to provide reference for clarifying the promoting blood circulation to restore menstrual flow traditional efficacy of L.chuanxiong. METHODS: The isolated uterine smooth muscle of rats were collected and soaked in Locke's solution. Using dimethyl sulfoxide as blank control, verapamil hydrochloride as positive control, the contraction curves of the isolated uterine smooth muscle in rats were recorded with PowerLab electrophysiolograph. Inhibitory effects of total alkaloid, total phenolic acid (mass concentrations of 0. 025, 0. 05, 0. 1 mg/mL) and total volatile oil (0. 04, 0. 08, 0. 16 mg/mL) on the contraction of the isolated uterine smooth muscle of rats induced by oxytocin were evaluated by observing the changes of contraction activity and contraction tension. Inhibitory rates of contraction activity and contraction tension of uterine smooth muscle were calculated. RESULTS: The contraction activity and contraction tension of uterine smooth muscle in rats were increased significantly after treated with oxytocin (P<0. 05 or P<0. 01). Different concentrations of 3 types of compounds from L. chuanxiong all decreased contraction activity and contraction tension of uterine smooth muscle significantly (P<0. 05 or P<0. 01). Compared with blank control, except for 0. 025 mg/mL total phenolic acid, the inhibitory rates of contraction activity and contraction tension of uterine smooth muscle were all decreased significantly (P<0. 05 or P<0. 01)after treated with other concentration drugs or components. CONCLUSIONS: The total alkaloid, total phenolic acid and total volatile oil from L. chuanxiong show certain inhibitory effect on the contraction of isolated uterine smooth muscle in rats, which provide reference for promoting blood circulation to restore menstrual flow use of L. chuanxiong.
Résumé
OBJECTIVE:To optimize the extraction technology of total volatile oil from Cinnamomum cassia Presl. and Cyper-us rotundus L. in Hegan lipi granules. METHODS:With the extraction rate of total volatile oil as the index,Box-Behnken response surface method was applied to investigate the effects of water amount,soaking time for medicinal materials and extraction time by distillation on the amount of the extracted volatile oil and optimize the extraction technology of total volatile oil from C. cassia Pre-sl. and C. rotundus L. in Hegan lipi granules,and verification tests were conducted. RESULTS:The relevant coefficient of the es-tablished quadratic polynomial regression model of 3 variable factors was 0.970 5. The optimal extraction technology was as follows as 5 times as much as the amount of medicinal materials of water,soaking time of 2.5 h,extraction time of 6.0 h. Verification tests showed the extraction rate of total volatile oil was 1.65%,with a deviation rate lower than 2% compared to the predicted value of 1.67%. CONCLUSIONS:The established model is reliable with good predictability. The optimal technology can be used for the ex-traction of total volatile oil from C. cassia Presl. and C. rotundus L. in Hegan lipi granules.