Résumé
BACKGROUND: Recent studies have shown that TCM massage can alleviate or even reverse the progression of muscle fibrosis, so it is speculated that massage can also improve the pathological changes of gluteal muscle contracture. OBJECTIVE: To evaluate the role of massage on gluteal muscle contracture and the relevant mechanism. METHODS: Twenty young rabbits were divided into an experimental group and a control group, with penicillin and benzyl alcohol injection for 8 weeks respectively. Besides, the experimental group received massage therapies for 4 weeks, whereas the control group had no specific intervention. Cell morphology and structure were observed using hematoxylin and eosin staining, Masson staining and Sirius red staining. Expression of transforming growth factor-pi in the rabbit model of gluteal muscle contraction was measured using immunohistochemistry, reverse transcription PCR and western blot assays. The study protocol was approved by the Ethics Committee of Peking University Shenzhen Hospital in China. RESULTS AND CONCLUSION: The experimental group had significantly less hyperplasia of fiber tissue compared with the control group (F < 0.05). The expression levels of collagen type I and transforming growth factor-31 were significantly increased in the control group compared with the experimental group, as determined by reverse transcription PCR, western blot assay and immunohistochemical staining (P < 0.05). Therefore, massage therapy can alleviate the progression of gluteal fibrosis, which is expected to provide a new idea for the research and treatment of gluteal muscle contracture.
Résumé
BACKGROUND: At present, scholars at home and abroad try to promote the growth of bone graft by changing the types and morphology of implant materials, and improving the thickness and smoothness of induced membrane. OBJECTIVE; To compare the degree of vascularization and the expression of some osteogenic factors in the polymethylmethacrylate bone cement membrane with different surface roughness degrees at different femoral bone defects in rats. METHODS: Forty-eight male Sprague-Dawley rats (purchased from the Experimental Animal Center of Guangzhou University of Chinese Medicine) were used to establish rat models of critical-size femur defect. These rat modes were then randomly divided into groups A, B, C and D. Polymethylmethacrylate bone cement occupiers with surface roughness 0.05). Immunohistochemical staining revealed that the positive expression of bone morphogenetic protein 2, transforming growth factor (31 and vascular endothelial growth factor protein was similar among four groups (P>0.05). These findings suggest that the change of surface roughness of bone cement has no significant effect on the histomorphological structure of induced membrane and the expression of bone morphogenetic protein 2, transforming growth factor pi and vascular endothelial growth factor in induced membrane at 6 weeks after implantation of bone cement spacer.
Résumé
BACKGROUND: Studies have found that polymethylmethacrylate bone cement implanted in the subcutaneous region, muscle and other parts can form induced membrane. The induced membrane favors vascularization and secretes multiple osteogenic factors. OBJECTIVE: To investigate the differences in the level of vascularization and the expression of osteogenic factors in the induced membrane among subcutaneous region, muscle and femoral bone defects. METHODS: Thirty-six male SD rats (purchased from the Experimental Animal Center of Guangzhou University of Chinese Medicine) were randomly divided into three groups (n=12/group). Polymethylmethacrylate antibiotic cement spacers were implanted in the subcutaneous tissue, muscle and femoral bone defects of the hind limbs. At 6 weeks after implantation, the induced membrane around the bone cement was removed. The change in membrane morphology was observed by hematoxylin-eosin staining. Expression of bone morphogenetic protein-2, transforming growth factor-31 and vascular endothelial growth factor in the induced membrane was detected by western blot, RT-qPCR and immunohistochemistry. This study was approved by Animal Ethics Committee, Guangzhou University of Chinese Medicine, China (approval No. 20181101006). RESULTS AND CONCLUSION: Hematoxylin-eosin staining showed that induced membrane formed in each group. The number of vessels in the outer layer of the membrane tissue section was higher in the femoral bone defect groups than that in the muscle and subcutaneous tissue group. The number of fibroblasts and myofibroblasts in the inner layer close to the bone cement was higher in the femoral bone defect group than in the muscle and subcutaneous tissue groups. Immunohistochemical staining revealed that bone morphogenetic protein-2, transforming growth factor-31 and vascular endothelial growth factor expression levels were highest in the femoral bone defect group and lowest in the subcutaneous tissue group. Western blot and RT-qPCR showed that bone morphogenetic protein 2, transforming growth factor 31 and vascular endothelial growth factor expression levels were significantly higher in the femoral bone defect group than in the muscle and subcutaneous tissue groups (P < 0.001). These findings suggest that different surrounding tissue conditions have different important influences on tissue structure and osteogenic factor expression in the induced membrane. Polymethylmethacrylate bone cement implanted in the femoral bone defects can improve the quality of induced membrane formation, lead to a larger amount of newly formed vessels and higher osteogenic factor expression in the induced membrane.