RÉSUMÉ
Rapeseed-mustard is one of the major oilseed crops cultivated in India. Alternaria brassicae is the most destructive pathogen of oilseeds. The present study aims to evaluate the effect of bio-agents and elicitors on Alternaria brassicae of mustard in vitro using dual culture technique and poison food technique in completely randomized design (CRD). The study was conducted at the Department of Plant Pathology Laboratory, Sam Higginbottom University of Agriculture, Technology And Sciences, Prayagraj. The results revealed that among bio-agents, Trichoderma viride + Pseudomonas fluorescens (87.70mm) recorded the maximum per cent inhibition of mycelial growth of Alternaria brassicae, followed by Trichoderma viride (82.60mm). Among elicitors, the maximum per cent inhibition of the pathogen was recorded by salicylic acid at 150 ppm (75.33%), followed by salicylic acid at 100 ppm (58.69%).
RÉSUMÉ
A survey was conducted during Rabi 2020 - 2021 to know the severity of Anthracnose of chilli in fields ofeight villages in Krishna district of Andhra Pradesh. The disease severity ranged from 13.30 to 29.65 per cent irrespective of location surveyed. The disease severity was least in Tadikilapudi village (13.3%) and highest in kamavarapukota village (29.65%) during the month of December 2020. To manage the disease severity evaluate the efficacy of bio-agents viz.., Seed Treatment with Pseudomonas fluorescens, Trichodermaviride and Microalgae.The foliar application of Neem oil is given to all the treatments. Among the Treatments disease intensity (%) was significantly decreased in treatment T6 - Pseudomonas fluorescens + Trichodermaviride + microalgae (16.03%). The plant height (cm) was significantly increased in treatment T6 – Pseudomonas fluorescens + Trichodermaviride + microalgae (62.2 cm) respectively, fruit length (cm) was significantly increased (15.96cm), yield was significantly increased in treatment T6 (128.33 q/ha) as compared to control T0.
RÉSUMÉ
A field experiment entitled, Influence of different organic sources of nutrients on yield and quality of pomegranate (Punica granatum L.) cv. Bhagwa. The current study was designed in RBD with three replications consisting of twenty-two treatments with four different organic manures viz., farmyard manure, vermicompost, poultry manure and neem cake as a source of nitrogen and recommended dose of manure and fertilizers based on plant age with or without biofertilizers (Azotobacter, PSB & KMB) and biopesticides (Trichoderma viride and and Paecilomyces lilacinus). The findings based on pooled data discovered that maximum number of fruits per plant (59.50), yield per plant (12.41 kg), yield per hectare (19.86 tonne), fruit weight (239.39 g), fruit diameter (7.55 cm), aril weight per fruit (147.70 g), juice content per fruit (97.55 ml), peel weight per fruit (91.69 g) along with peel: aril ratio (0.62) was significantly noted under treatment 100 % RDN through poultry manure + 50 ml PSB + 25 ml KMB + 5 g Trichoderma viride + 5 ml Paecilomyces lilacinus. The maximum net return (4,12,840/ha) and BCR (3.26) were also obtained in same treatment. Significantly maximum TSS (16.15°Brix), reducing sugar (14.44 %), non-reducing (2.04 %) and total sugar (16.48 %) along with minimum titrable acidity (0.41 %) were recorded with application of 37.5 % RDN through FYM + 37.5 % RDN through neem cake + 50 ml Azotobacter culture + 50 ml PSB + 25 ml KMB + 5 g Trichoderma viride + 5 ml Paecilomyces lilacinus treatment. Whereas, maximum ascorbic acid content (16.72 mg/100 ml juice) was significantly found under treatment 100 % RDN through FYM + 50 ml PSB + 25 ml KMB + 5 g Trichoderma viride + 5 ml Paecilomyces lilacinus.
RÉSUMÉ
Aims: Sclerotium rolfsii is a pathogen of agricultural crops such as tomatoes. Trichoderma viride has been shown to control this pathogen effectively but the optimum conditions of this biocontrol agent need to be determined. This piece of research was therefore aimed at studying the conditions that could be maintained in maximizing the full potential of T. viride. Methodology and results: Malt extract agar was separately supplemented with different carbon sources (glucose, sucrose, maltose, lactose, and mannitol), nitrogen sources (peptone, ammonium nitrate, zinc nitrate and sodium nitrate) as well as salts (NaCl, CaCl2, KI, BaCl2) at different concentrations. Effect of different temperature and pH ranges was also evaluated. All the carbon used supported the growth of both S. rolfsii and T. viride at all concentrations. The growth of S. rolfsii was less than 90 mm at 3% of all the carbon used compared to the plates in which no carbon sources were added whereas the growth of T. viride was 90 mm. There were morphological changes in the growth of both T. viride and S. rolfsii with increase in concentrations of peptone and ammonium nitrate. The greenish colour of T. viride was completely lost while S. rolfsii became fluffier. For peptone, the percentage reduction ranged from 68.52 to 63.33 while for zinc nitrate and sodium nitrate, the percentage reduction ranged from 85.19 to 80.74 and 55.00 to 43.70 respectively. Also, there was increase in antagonistic property of T. viride with the inclusion of salts (NaCl, CaCl2 and KI) at all concentrations used. However, barium chloride was toxic to T. viride at 2-5% (w/v). The optimum temperature and pH for the antagonistic property of T. viride appeared to be 37 °C and 6.0 respectively. Conclusion, significance and impact of study: Inclusion of different carbon, nitrogen and salts increased the antagonistic activities of T. viride against S. rolfsii although lactose appeared to be the best carbon source while zinc nitrate and CaCl2 were the best nitrogen and salt respectively. Barium chloride was toxic to T. viride at 2-5% (w/v).
RÉSUMÉ
Aims: The escalating demands for traditional fossil fuels with unsecured deliverance and issues of climate change compel the researchers to develop alternative fuels like bioethanol. This study examines the prospect of biofuel production from high carbohydrate containing lignocellulosic material, e.g. sugarcane bagasse through biological means. Methodology and Results: Cellulolytic enzymes were collected from the culture filtrate of thermotolerant Trichoderma viride grown on variously pre-treated sugarcane bagasse. CMCase and FPase enzyme activities were determined as a measure of suitable substrate pre-treatment and optimum condition for cellulolytic enzyme production. The highest CMCase and FPase activity was found to be 1.217 U/ml and 0.109 U/ml respectively under the production conditions of 200 rpm, pH 4.0 and 50 °C using steamed NaOH treated bagasse as substrate. SEM was carried out to compare and confirm the activity of cellulolytic enzymes on sugarcane bagasse. Saccharification of pre-treated bagasse was carried out with crude enzymes together using a two-factor experimental design. Under optimized conditions the pre-treated bagasse was saccharified up to 42.7 % in 24 h. The hydrolysate was concentrated by heating to suitable concentration and then used for fermentation by an indigenous isolate of Saccharomyces cerevisiae. With 50 and 80 % brix containing liquor the concentration of alcohol was 0.579 % and 1.15 % respectively. Conclusion, significance and impact of study: This is the first report in Bangladesh for the production of cellulosic ethanol using local isolates. Though the rate of alcohol production was very low, a great impetus in this field can maximize the production thereby meet the demand for fuel in future.
RÉSUMÉ
In the present study, cultural and nutritional conditions for enhanced production of xylanase by a local soil isolate of Trichoderma viride, using various lignocellulosic substrates in submerged culture fermentation have been optimized. Of the lignocellulosics used, maize straw was the best inducer followed by jowar straw for xylanase production. The highest activity achieved was between 14 to 17 days of fermentation. A continuous increase in xylanase production was observed with increasing level of lignocellulosics in the medium and highest activity was observed with maize straw at 5 percent level. Xylanase production with higher levels of lignocellulosics (3 to 5 percent) of maize, jowar and barseem was found to be higher as compared to that with commercial xylan as carbon source. Sodium nitrate was the best nitrogen source among the six sources used. Maximum xylanase production was achieved with initial medium pH of 3.5-4.0 and incubation temperature of 25ºC.The enzyme preparation was effective in bringing about saccharification of different lignocellulosics. The xylanase production could be further improved by using alkali treated straw as carbon source.
Neste estudo, otimizou-se as condições culturais e nutricionais para produção aumentada de xilanase por uma cepa local de Trichoderma viride isolada de solo, empregando-se vários substratos lignocelulósicos, em fermentação submersa. Entre os substratos utilizados, o melhor indutor de produção de xilanase foi palha de milho, seguido de palha de sorgo. A atividade mais alta foi obtida entre 14 e 17 dias de fermentação. Com palha de milho observou-se um aumento contínuo na produção de xilanase com o aumento da concentração dos substratos lignocelulósicos no meio, sendo que a melhor atividade foi obtida com 5 por cento de palha de milho. A produção de xilanase com níveis mais altos de (3 a 5 por cento) de milho, sorgo e forragem verde (barseem) foi mais levada do que com xilana comercial como fonte de carbono. Entre as fontes de nitrogênio testadas, a melhor foi nitrato de sódio. Produção máxima de xilanase foi obtida quando o pH inicial do meio foi 3,5 4,0 e a temperatura de incubação 25ºC. A enzima foi eficiente na sacarificação de diferentes substratos lignocelulósicos. A produção de xilanase poderia ser aumentada empregando-se álcali ao invés de palha tratada como fonte de carbono.
Sujet(s)
Techniques in vitro , Azote , Microbiologie du sol , Sorghum , Trichoderma/isolement et purification , Xylanes/analyse , Fermentation , Méthodes , Substrats pour Traitement BiologiqueRÉSUMÉ
The effects of surfactants on the production of cellulase by Trichoderma viride in liquid substrate fermentation process were investigated. Straw was used as the sole carbon source and the surfactants were biosurfactant rhamnolipid from Pseudomonas aeruginosa and Tween 80. The changes of FPA,CMCase,Avicelase and surface tension with time were analyzed under different concentrations of the two surfactants. The results showed that the surfactants can enhance the enzyme activity of Trichoderma viride. The FPA,CMCase,Avicelase were promoted 1.08,1.6 and 1.03 times higher than the controls by rhamnolipid. The enhancement of the enzyme activity by rhamnolipid was much higher than that of Tween 80. At the same time,rhamnolipid was not degraded prior to other substrate.
RÉSUMÉ
To study the chemical components and the antifungal activity of extraction from conidia of Trichoderma viride LTR-2.The extraction were obtained by distilling with Methylene dichloride from conidia of Trichoderma viride LTR-2 cultured on wheat bran solid matrix.Antifungal activity were determined by mycelium growth method.The chemical components of the extraction were analysed by GC-MS,the relative components in the extraction were determined by area normalization.The extraction not only have broad-spectrum control,showed antibiosis against eleven different plant fungal pathogens in PDA dish,such as Rhizoctonia solani,Alternaria brassica,Verticillium dahliae,Macrophoma kawatsukai,Fusarium moniliforme,Botrytis cinerea,Rhizoctonia cerealis,Fusarium oxysporum f.sp.vasinfectum,Bipolaris sorokinana,Fusarium graminearum,Alternaria.mali,but also have high inhibitory effect,and had 89.3% suppressive rate to Rhizoctonia cerealis.About sixty components were separated and identified by GC-MS,majority components were Hydrocarbon,the number of the Hydrocarbon were fourty-three kinds.Ergosterol was the major chemical components of the extract,and has 41.90% content.Other components comprised:Ketone,Organic acid,Alcohol,Ene,et al.Conclusion:The extraction from conidia of Trichoderma viride LTR-2 have antifungal activity.The extration comprised 2H-Pyran-2-one,5,6-dihydro-6-pentyl,it has 2.35% content.reference others literature,2H-Pyran-2-one,5,6-dihydro-6-pentyl may be the suppressive component of the extration.