Clinical value of NKT cells and tumor abnormal proteins in stage Ⅲ-Ⅳ B head and neck squamous cell carcinoma / 中华放射医学与防护杂志

Objective:To investigate the clinical value of changes in peripheral NKT cells and tumor abnormal proteins (TAPs) in stage Ⅲ-Ⅳ B head and neck squamous cell carcinoma (HNSCC) before and after radiotherapy. Methods:A retrospective analysis was performed using the data of 101 HNSCC patients, who were confirmed from January 2019 to December 2021 and treated with radical and postoperative radiotherapy. Flow cytometry and the agglutination method were used to determine the proportion of NKT cells in peripheral blood and the TAP coagulation area, respectively before and after radiotherapy. The relationships of clinical features and the cellular features such as changes in NKT cells and ATPs with local recurrence and long-term survival were analyzed. The χ2 test or Fisher′s exact test was employed for intergroup comparison. The Kaplan-Meier method and the Cox model were utilized for univariate and multivariate survival prognosis analyses, respectively. The bivariate Pearson linear correlation analysis was conducted to analyze the relationship between NKT and TAP. Results:The median follow-up time of the whole group was 25 months. Regarding the 1-, 2-, and 3-year survival rates, the local-regional recurrence-free survival (LRRFS) rates were 76.2%, 67.3%, and 64.4%, respectively, the distant metastasis-free survival (DMFS) rates 91.1%, 90.1%, and 89.1%, respectively, and the progression-free survival (PFS) rates 69.3%, 59.4%, and 55.4%, respectively. The 3-year overall survival (OS) rate was influenced by age, surgery, N stage, TNM stage, NKT cell ratio, and TAP, while the 3-year PFS rate was affected by TAP, sex, N stage, and TNM stage. Multivariate analysis suggests that independent adverse prognostic factors for HNSCC included sex, age, N stage, NKT cells, and TAP ( HR=3.00, 2.35, 2.27, 2.02, 2.56, P<0.05). The correlation analysis indicates a positive correlation between NKT cells and TAP ( r=0.26, P=0.009). Conclusions:Stage Ⅲ-Ⅳ B HNSCC treated with radical and postoperative radiotherapy is subjected to a high recurrence rate. Further research is required for the expression levels of NKT cells and TAP in peripheral blood, as well as the influence of their changes during radiotherapy on the 3-year OS, PFS, and LRRFS rates of locally advanced HNSCC.

The value of abnormal protein and C-reactive protein levels in predicting human papillomavirus infection and cervical lesions in patients with peripheral blood tumors / 中国基层医药

Objective:To investigate the value of abnormal protein (TAP) and C-reactive protein (CRP) levels in the prediction of human papillomavirus (HPV) infection and cervical lesions in patients with peripheral blood tumors.Methods:A total of 216 patients with cervical lesions who received treatment in Shan County Central Hospital from June 2019 to May 2021 were included in this cross-sectional study. According to HPV results, these patients were divided into a high-risk HPV infection group ( n = 122), a low-risk HPV infection group ( n = 76), and an HIV-uninfected group ( n = 18). TAP and CRP levels in peripheral blood were determined in each group and analyzed. Results:TAP and CRP levels in the high-risk HPV infection group were (243.29 ± 19.43) μm 2 and (37.49 ± 10.50) mg/L, respectively, and they were (125.46 ± 10.37) μm 2 and (26.27 ± 7.28) mg/L in the low-risk HPV infection group, and (74.51 ± 9.29) μm 2 and (74.51 ± 9.29) mg/L in the HIV-uninfected group. There were significant differences in TAP and CRP levels among the three groups ( F = 12.40, 6.34, both P < 0.001). TAP and CRP levels in the low-risk HPV infection group were significantly higher than those in the HIV-uninfected group ( t = 4.02, 3.26, both P < 0.05). TAP and CRP levels in the peripheral blood of patients with chronic cervicitis were (63.25 ± 5.80) μm 2 and (13.37 ± 5.29) mg/L, which were significantly different from those in patients with cervical intraepithelial neoplasia or cervical cancer ( F = 21.35, 8.26, both P < 0.05). TAP and CRP levels in the peripheral blood of patients with cervical intraepithelial neoplasia grade II and cervical intraepithelial neoplasia grade III were significantly higher than those in patients with cervical intraepithelial neoplasia grade I (both P < 0.05). TAP and CRP levels in the peripheral blood of patients with cervical cancer were significantly higher than those in patients with various grades of cervical intraepithelial neoplasia (all P < 0.05). The Pearson correlation analysis results showed that the load of high-risk human papillomavirus deoxyribonucleic acid (HPV-DNA) was positively correlated with blood TAP and CRP levels ( r = 0.64, 0.24, both P < 0.001). The area under the curve regarding the combined detection of TAP and CRP levels was 0.927 (95% CI: 0.873-0.967), with sensitivity and specificity of 91.0% and 93.6%, respectively. Conclusion:TAP and CRP levels in peripheral blood can reflect HPV infection and cervical lesion and are closely related to the type of HPV infection and the degree of cervical lesion. Combined detection of the two can improve the diagnostic efficiency of cervical cancer and deserves clinical promotion.

Expression and clinical significance of tumor abnormal protein in breast cancer / 中华内分泌外科杂志

Objective To explore the clinical application value of serum tumor abnormal protein (TAP)in early diagnosis of breast cancer.Methods The serum TAP level was determined in 53 hospitalized patients with breast cancer and 65 cases of normal physical examination population as the control group.We further compared the positive rate of TAP in the two groups and the expression level of TAP between different clinical pathological parameters in breast cancer group.Results There was no case of TAP positive in the control group,while the positive rate of TAP was as high as 83.02％ in breast cancer group.TAP positive rate of the patients with negative PR (100.00％) was significantly higher than that of PR positive patients (73.53％)(P=0.019).However there was no significant difference of TAP positive rate between patients with different ages,clinical stages,lymph node metastasis and the different expression of ER,C-erbB2 and Ki67.Conclusion It might be clinically valuable to use TAP expression level as a screening marker for breast cancer in combination with the breast cancer hormone PR.

Values of SCCAg, TAP and CEA in evaluating the efficacy of neoadjuvant chemotherapy for cervical cancer / 国际肿瘤学杂志

Objective To investigate the values of squamous cell carcinoma antigen (SCCAg),tumor abnormal protein (TAP),carcinoembryonic antigen (CEA) in evaluating the efficacy of neoadjuvant chemotherapy for cervical cancer.Methods A total of 100 patients with cervical cancer treated by neoadjuvant chemotherapy were selected from September 2015 to September 2017 in our hospital,and 100 healthy persons were selected as the control group at the same time.The serum levels of SCCAg,TAP and CEA were detected and the relationships between the levels of SCCAg,TAP,CEA and the efficacy of neoadjuvant chemotherapy were analyzed.Results The serum levels of SCCAg [(4.95 ±0.65) μg/L vs.(0.22 ±0.04) μg/L],TAP [(175.21 ± 25.42) μm2 vs.(75.45 ± 9.98) μm2],CEA [(35.65 ± 4.23) ng/ml vs.(1.26 ± 0.34) ng/rnl] in patients with cervical cancer were significantly higher than those of control group,and the differences were statistically significant (t =75.382,P ＜ 0.001;t =62.215,P ＜ 0.001;t =55.452,P ＜ 0.001).Three months after neoadjuvant chemotherapy for 100 cervical cancer patients,complete remission (CR) was achieved in 6 cases (6.00％),partial remission (PR) in 50 cases (50.00％),stable disease (SD) in 26 cases (26.00％),and progression disease (PD) in 18 cases (18.00％).The SCCAg,TAP and CEA levels of patients with CR [(2.12 ± 0.32) μg/L vs.(4.90 ± 0.52) μg/L,(133.12 ± 14.22) μm2 vs.(175.12 ± 24.32) μm2,(10.34 ± 2.42) ng/ml vs.(38.21 ± 7.82) ng/ml] and PR after chemotherapy were significantly lower than those before chemotherapy [(3.22 ± 0.47) μg/L vs.(4.94 ± 0.53) μg/L,(145.22 ± 17.77) μm2 vs.(179.52 ± 25.53) μm2,(16.75 ± 3.02) ng/ml vs.(39.12 ± 7.92) ng/ml],and the differences were statistically significant (t =11.153,P ＜ 0.001;t =3.562,P =0.004;t =8.340,P ＜ 0.001;t =17.169,P ＜ 0.001;t =7.797,P ＜ 0.001;t =18.662,P ＜ 0.001).The above indicators of patients with PD after chemotherapy were significantly higher than those before chemotherapy [(7.21 ± 0.84) μg/Lvs.(5.06±0.57) μg/L,(213.21 ±29.64) μm2vs.(171.56±26.87) μm2,(46.64± 5.12) ng/ml vs.(35.75 ± 7.88) ng/ml],and the differences were statistically significant (t =8.986,P ＜ 0.001;t =4.417,P ＜0.001;t =4.917,P ＜0.001).The differences of the above indicators before and after chemotherapy in patients with SD were not statistically significant [(5.03 ± 0.57) μg/L vs.(4.97 ± 0.55) μg/L;(175.51 ± 23.37) μm2 vs.(176.27 ± 26.55) μm2;(35.26 ± 7.34) ng/ml vs.(37.04 ± 7.73) ng/ml;t =0.386,P=0.701;t=0.110,P=0.913;t=0.851,P=0.399].The results of receiver operating characteristic (ROC) curve analysis showed that the sensitivity,specificity and accuracy of SCCAg in evaluating the neoadjuvant chemotherapy for cervical cancer were 85.71％,81.82％,84.00％,those of TAP were 82.14％,77.27％,80.00％,those of CEA were 78.57％,77.27％,78.00％,and those of the combined detection were 96.43％,95.45％,96.00％.The sensitivity,specificity and accuracy of the combined detection were significantly higher than those of the three alone,and the differences were statistically significant (x2 =14.434,P＜0.001,x2 =15.421,P＜0.001,x2 =21.741,P＜0.001).Conclusion The serum levels of SCCAg,TAP and CEA in patients with cervical cancer are decreased after neoadjuvant chemotherapy.Their level changes can be used as important indicators to evaluate the efficacy of chemotherapy,and the combination of the three has better evaluation efficiency,which is worth for further clinical promotion.

Correlation between serum tumor abnormal protein expression and chemotherapeutic effect in patients with lung adenocarcinoma / 肿瘤研究与临床

Objective To investigate the correlation between serum tumor abnormal protein (TAP) a nd chemotherapeutic effect in patients with lung adenocarcinoma. Methods A prospective study was conducted on 90 patients with lung adenocarcinoma Liyuan Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology from May 2012 to April 2016. The patients were divided into adjuvant chemotherapy group (30 cases) and palliative chemotherapy group (60 cases) according to the different treatment regimen. The adjuvant chemotherapy group was treated with FOLFOX regimen for postoperative adjuvant chemotherapy. The palliative chemotherapy group was treated with pemetrexed. The t test was used to compare the expression levels of TAP in both groups. The χ2 test was used to compare the relationship among expression of TAP, the positive results of tumor markers and the efficacy of chemotherapy. Pearson correlation analysis was used to analyze the expression level of TAP and changes of tumor markers. Results There was no statistically significant difference in serum TAP between the two groups before palliative chemotherapy [area of TAP condensed matter were (230±80)μm2 and (206±50) μm2, t=1.487, P<0.05]. The serum TAP expression in both groups was 100％. There was no statistically significant difference in serum TAP area between adjuvant chemotherapy group and palliative chemotherapy group (46.7 ％ vs. 48.3％, χ2= 0.022, P> 0.05). There was no statistically significant difference between the two groups in positive expression of tumor markers CA125, CEA, CA19-9 and ALP (χ2 values were 4.02, 3.81, 4.01, 5.01, all P>0.05). In the two groups, the changes of serum TAP before and after chemotherapy had no relationship with age, gender, pathological differentiation degree and the number of transfer viscera (all P>0.05). The expression of serum TAP, CEA and CA19-9 after chemotherapy in palliative chemotherapy group were related to the efficacy of chemotherapy (χ2=6.62, 7.78, 8.62, all P<0.01). Pearson correlation analysis showed that the changes of serum TAP in palliative chemotherapy group had no relationship with tumor markers CA125, CEA, CA19-9 and ALP (r values were 0.42, 0.19, 0.09, 0.28, all P> 0.05). The serum TAP level of the adjuvant chemotherapy group was positively correlated with the level of tumor marker CA125 (r=0.51, P=0.02), with no correlation with the changes of CEA, CA19-9 and ALP levels (r values were 0.20, 0.24, 0.19, all P>0.05). Conclusion The detection of serum TAP level in patients with lung adenocarcinoma has good accuracy and high sensitivity, which indicates that the serum level of TAP in patients with lung adenocarcinoma before and after chemotherapy can be used as an index to evaluate the efficacy of chemotherapy and an independent detection of lung adenocarcinoma.

Significance of Tumor Abnormal Protein in Peripheral Blood in the Therapeutic Monitoring of Non-Small Cell Lung Cancer / 现代检验医学杂志

Objective To observe the significance of tumor abnormal protein (TAP)in the therapeutic monitoring of non-small cell lung cancer (NSCLC).Methods Peripheral blood from 30 NSCLC patients were collected to make smears at the moment of pre-treatment,half a month,one month,3 months and 6 months after therapy.TAP was detected by coacervation method.The maximum area of condense was applied to estimate the level of TAP.Thirty healthy subj ects were chose as con-trol group.Results The positive rate of TAP in NSCLC patients was 86.67%,and 3.33% of healthy subjects were positive in TAP.The difference was statistically significant (χ2=140.3,P<0.01).The condense area of TAP in patients withⅢ~Ⅳ stage of NSCLC [411(89,562)mm2]was significantly higher than those withⅠ~Ⅱ stage NSCLC [267(31,407)mm2]. The condense areas in both of two groups went down after treatment.A significant difference of condense area appeared inⅠ~Ⅱ stage of NSCLC patients a month after therapy as well as Ⅲ~Ⅳ stage of NSCLC patients half a month after treat-ment.The condense areas went to their lowest level 3 months after therapy.ForⅠ~Ⅱ stage patients,the condense area fell to 21% compared to that before treatment,but for patients withⅢ~Ⅳ stage of NSCLC,37% of pre-treatment condense ar-ea were detected.TheⅠ~Ⅱ stage of NSCLC patients had a greater decrease in condense area of TAP than theⅢ~Ⅳ stage patients by 3 months after treatment (χ2=6.22,P<0.05).Conclusion Detection of TAP in peripheral blood had a high sensitivity for NSCLC,and is able to monitoring the treatment effect.

Expression and clinical significance of TAP in lung cancer patients / 实用医学杂志

Objective To investigate the expression and clinical significance of tumor abnormal protein (TAP)in patients with lung cancer.Methods Plasma TAP concentration was measured by enzyme-linked immu-nosorbent assay(ELISA)in 93 patients with lung cancer and 100 healthy subjects.Results The plasma TAP con-centration[(187.71 ± 82.295)μm2]in lung cancer group was significantly higher than that in the healthy control group[(67.836 ± 28.642)μm2,t=13.991,P<0.05).The sensitivity of TAP in lung cancer group was 83.87%. Conclusions TAP can improve the early diagnosis rate of lung cancer patients,which is important for early diag-nosis and early treatment. TAP detection is suitable for lung cancer screening in healthy people and people with high risks.

The si gnificances tumor abnormal p rotein detection for diagnosis and prediction of prognosis in gastroin-tetsinal tumors / 实用肿瘤学杂志

Objective To investigate the possibility of tumor abnormal protein ( TAP) ,as index of diag-nosis and prediction of prognosis in gastrointestinal tumors (GITs).Methods The outpatients and inpatients as well as healthy physical examinees in our hospital were chosen as objects in the present study .The patients were divided into GIT group(120 cases),high-risk GIT group(123 cases)and normal group(117 cases).TAP ex-pression was detected in three groups.These study objects were followed up for one and a half years .Then the re-lationship between TAP expression and the occurrence or recurrence of tumors was analyzed .Rseults There were significant differences(P<0.001)among the three groups on the positive TAP with critical expression rate .TAP detections to GITs diagnosis sensitivity ,specificity ,positive predictive value ,negative predictive value and Youden index were 88.33%,85.83%,75.71%,93.64% and 0.74 respectively.TAP positive non GITs crowd tumori-genesis proportion (23.53%)was significantly higher than that of TAP -negative(0.49%)(P<0.001).GITs TAP positive patients relapse rate(33.33%)was significantly higher than negative ones (6.90%)(P<0.001). Conlcusion TAP can be an index for diagnosis ,early prevention ,monitoring of treatment effect and prediction of prognosis of gastrointestinal tumor .