Effect of Intrathymic Injection of Allogene Bone Marrow on Small Intestine Transplantation of Rats / 中国康复理论与实践

@#ObjectiveTo investigate the immune tolerance function and significance of allogene bone marrow injection to the small intestines transplantation of rats.MethodsInbreeding line rat F344/N and Wistar/A were selected to perform heterotopic graft of the whole small intestine. 7 days before allogene transplantation, donator bone marrow cells (BMC) were injected into thymus of acceptor (the testing group). According to the isogene and allogene rat transplant model, it was comprehended whether injecting allogene donator marrow into acceptor thymus could decrease the acute rejection after transplantation.Results3, 5 or 7 days after allogeneic rat dystopia whole small intestine transplantation, typical reject reaction appeared, but there was no reject reaction in isogenome and testing group. 3 days after allotransplantation, serum soluble interleukin-2 receptor (sIL-2R) and tumor necrotic factor-α (TNF-α) levels were significantly higher than the other groups (P<0.01). The level of serum sIL-2R and TNF-α in the allogene marrow injecting group were only slight higher on the 3rd or 5th day, and getting downtrend, and there was no significant difference compared with isogenic transplantation group.ConclusionAllogenic donator bone marrow intrathymic injecting into acceptor 7 days before small intestina transplantation, can reduce the reject reaction after the grafting. The levels of serum sIL-2R and TNF-α can be selected as a sensitive early diagnosis index of acute rejection after small intestine transplantation.

Role of Overexpressed Proinflammatory Cytokines in the Pathogenesis of Cerebral Palsy / 中国康复理论与实践

@#ObjectiveTo investigate the role of overexpressed proinflammatory cytokines in the pathogenesis of cerebral palsy (CP).MethodsLevels of tumor necrotic factor-α (TNF-α) and interleukin-6 (IL-6) in serum of 31 CP children, 20 healthy children (as controls), 37 neonates with CP risk factors such as hypoxic-ischemic injury and/or perinatal infection, and 20 healthy neonates (as controls) were measured by enzyme-linked immunosorbent assays (ELISA) retrospectively.ResultsLevels of TNF-α and IL-6 of CP children and neonates with CP risk factors were significantly higher than that of healthy controls (P<0.05). TNF-α level of CP children was significantly higher than that of neonates with CP risk factors (P<0.05), but there was no significant difference in IL-6 level between two groups.ConclusionOverexpressed proinflammatory cytokines play an important role in the pathogenesis of CP and may be an independent risk factor of CP.

Association of polymorphism in the tumor necrotic factor-alpha (TNF-alpha) gene and interleukin-1beta (IL-1beta) gene with preterm delivery / 대한산부인과학회잡지

OBJECTIVE: To elucidate whether polymorphisms of tumor necrotic factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) are associated with preterm delivery caused by preterm labor and preterm premature rupture of membrane (PPROM) in korean pregnant women. METHODS: From August 2002 to October 2003, we recruited 93 pregnant women who delivered before 37 weeks in gestation. In 93 cases, there were 66 cases with preterm labor and 27 cases with PPROM. 288 pregnant women who delivered after 37 weeks in gestation served as controls. Maternal blood was collected and Genomic DNA was extracted from peripheral blood and the relevant genomic region was amplified using polymerase chain reaction (PCR). PCR products were digested with the restriction enzymes, Nco I for TNF-alpha and Ava I for IL-1beta. Data was analyzed by 2-tailed unpaired t test, x2 test, odds ratio with 95% confidence interval using SPSS 10.0. RESULTS: In normal pregnant group, the proportions of TNF-alpha*1/ TNF-alpha*1, TNF-alpha*1/ TNF-alpha*2, and TNF-alpha*2/ TNF-alpha*2 were 91%, 8%, and 1%, respectively. In normal pregnant group, the proportions of IL-1beta*A/IL-1beta*A, IL-1beta*A/IL-1beta*a, and IL-1beta*a/IL-1beta*a were 29%, 40%, and 31%,respectively. The carrier rate of TNF-alpha*2 was significantly different in PPROM group compared with control group (22% vs. 9%, P=0.03), while not in preterm labor group (15% vs. 9%, P=0.138). The allelic frequency of TNF-alpha*2 was significantly different neither in preterm labor group nor in PPROM group compared with control group (0.08 vs. 0.05, P=0.248; 0.11 vs. 0.5, P=0.062, respectively). The carrier rate of IL-1beta*a was significantly different neither in preterm labor group nor in PPROM group compared with control group (68% vs. 71%, P=0.671; 67% vs. 71%, P=0.65, respectively). The allelic frequency of IL-1beta*a was significantly different neither in preterm labor group nor in PPROM group compared with control group (0.44 vs 0.52, P=0.151; 0.52 vs. 0.52, P=0.512, respectively). CONCLUSION: These results suggest that TNF-alpha polymorphism may be, at least in part, associated with PPROM.

Inhibition effect on LPS-induced TNF-? release by homegrown and Australian Melaleuca alternifolia(Tea Tree)oil / 第三军医大学学报

Objective To compare the inhibition of the homegrown and Australian tea tree oil(TTO)solution on TNF-? release from RAW264.7 cells induced by LPS.Methods MTT assay was used to measure the effects of TTO on viability of RAW264.7 in vitro.RAW264.7 cells were pretreated with two kinds of TTO solution in the dose-and time-dependent manners,then stimulated by LPS.TNF-? in the supernate was tested by ELISA.Results In vitro,two kinds of TTO(