Establishment of tissue culture and rapid propagation system of Typhonium flagelliforme / 中草药

Objective: To establish the rapid propagation system and tissue culture techniques of Typhonium flagelliforme for large-scale seedlings. Methods: Using media with different hormones proportions, the tuber tissue was found to be a good explant for inducing asexual propagation system. Results: The callus culture medium consisted of MS+NAA 0.2 mg/L+KT 1.0 mg/L+sucrose 3%+agar 6 g/L could generate callus successfully. Medium consisted of MS+NAA 0.2 mg/L+6-BA 2 mg/L+sucrose 3% + agar 6 g/L was a superlative proportion of hormones concentration to generate adventitious buds efficiently, especially for higher proliferated multiples. The rooting medium consisted of 1/2 MS+NAA 0.2 mg/L+IB A 0.4 mg/L+KT 0.2 mg/L+sucrose 3%+agar 6 g/L+AC 0.3 g/L was conducive to generate roots rapidly. The expiants could be acclimatized after a period of 12 to 14 weeks. Experiments of one-step-seedling formation indicated that the one-step-seedling formation medium, containing MS+NAA 0.2 mg/L+6-BA 1.5 mg/L+sucrose 3%+agar 6 g/L was the best proportion of hormone concentration, for 5 to 6 weeks, new plantlets could be developed, which will be acclimatized in 10 weeks. Conclusion: The tissue culture techniques and rapid propagation system of T. flagelliforme could be used for large-scale seedlings and lay a foundation for its improved breeds.

IEF Electrophorogram of protein in Rhizoma Pinelliae / 中草药

Objective To discriminate Rhizoma Pinelliae from its relative species by electrophoro-gram of protein.Methods Using isoelectrofocusing(IEF) technology to compare the electrophorogram of tuber of Pinellia ternata produced in different habitats and from its relative plants then determining the pH values.Results The IEF electrophorogram of Rhizoma Pinelliae was stable with eight clear characteristic protein bands and had obvious differences from the electrophorogram of the tubers of its relative plants.Conclusion The IEF electrophoresis of Rhizoma Pinelliae can be used as the distinguishing basis.The PI value of the characteristic protein of P.ternana offers the basic parameter for separation and purification of the protein in Rhizoma Pinelliae.