Résumé
Genus Klebsiella from faeces of sloth bears was screened by using culture morphology, Gram’s staining, biochemical tests and polymerase chain reaction. Our results showed that out of 60 samples collected, 22 samples (36.67%) were cultured on Klebsiella Selective Agar Base with Klebsiella Selective Supplement and Gram’s stain revealed rod-shaped Gram-negative organism with purple-magenta colony - like colonies. The biochemical tests of cultured samples revealed negative to indole production and methyl red test, positive to Voges-Proskauer test, positive to Simmon citrate utilization test, negative to H2S production and that produced acid over acid reaction in TSI agar and positive to urea production in cultured samples. All Klebsiella species isolates were sensitive to azithromycin followed by enrofloxacin and resistant to clindamycin and methicillin. The gyrA gene was amplified by PCR for the genus Klebsiella and found to be positive of 36.67%. This study may provide information for developing strategies in the future in the control of Klebsiella species infections in sloth bears
Résumé
An example of lymphoblastic lymphosarcoma was found in a 7-year-old male brown bear (Ursus arctos) that died after having a 7-month history of depression, anorexia and watery diarrhea. Grossly the mesenteric lymph nodes were enlarged to approximately 4 to 6 times their normal size and histologically diagnosed as lymphoblastic lymphosarcoma. The small intestinal mucosa was corrugated and had severe mural thickening due to infiltrated neoplastic cells. Hepatic metastasis was also noted. This is the first reported case of lymphosarcoma in Ursidae in Korea. As an incidental finding, endogenous lipid pneumonia was noted in the lung.