Your browser doesn't support javascript.
loading
Montrer: 20 | 50 | 100
Résultats 1 - 1 de 1
Filtre
Ajouter des filtres








Gamme d'année
1.
Mem. Inst. Oswaldo Cruz ; 103(3): 301-305, May 2008. ilus, tab
Article Dans Anglais | LILACS | ID: lil-485225

Résumé

Three Enterococcus faecalis and one Enterococcus faecium strains were characterized by plasmid profile, pulsed-field gel electrophoresis (PFGE) and determination of antimicrobial minimal inhibitory concentrations. VanA elements were characterized by Long PCR, overlapping PCR and DNA sequencing. Enterococcal strains showed resistance to vancomycin and harbored the vanA gene, and three these were teicoplanin susceptible while one showed intermediate resistance to teicoplanin. Two E. faecalis strains showed indistinguishable PFGE profile while the third was unrelated. E. faecalis strains showed a deletion in the right terminal region of the Tn1546-like element. The E. faecium strain showed an insertion element in the vanXY intergenic region. Mutations in VanA elements were not found. Rearrangements in the VanA element could be responsible for incongruities in genotype and phenotype in these strains.


Sujets)
Humains , Antibactériens/pharmacologie , Enterococcus faecalis , Enterococcus faecium , Téicoplanine/pharmacologie , Vancomycine/pharmacologie , Brésil , Protéines bactériennes/génétique , Carbon-oxygen ligases/génétique , ADN bactérien/génétique , Électrophorèse en champ pulsé , Enterococcus faecalis/effets des médicaments et des substances chimiques , Enterococcus faecalis/génétique , Enterococcus faecalis/isolement et purification , Enterococcus faecium/effets des médicaments et des substances chimiques , Enterococcus faecium/génétique , Enterococcus faecium/isolement et purification , Génotype , Tests de sensibilité microbienne , Phénotype , Réaction de polymérisation en chaîne , Analyse de séquence d'ADN , Résistance à la vancomycine/génétique
SÉLECTION CITATIONS
Détails de la recherche