Pharmacological research progress of five classical prescriptions in treatment of chronic heart failure / 中国中药杂志

Chronic heart failure(CHF) is a comprehensive clinical syndrome caused by multiple factors that result in structural and/or functional abnormalities of the heart, leading to impaired ventricular contraction and/or relaxation functions. This medical condition represents the final stage of various cardiovascular diseases. In the treatment of CHF, multiple clinical studies have demonstrated the benefits of using traditional Chinese medicine(TCM) to control oxidative stress, inflammation, and apoptosis, thereby delaying ventricular remodeling and reducing myocardial fibrosis. In this study, common TCM syndromes in the diagnosis and treatment of CHF in recent years were reviewed and summarized. Five common treatment methods including benefiting Qi and activating blood circulation, enhancing Qi and nourishing Yin, warming Yang for diuresis, eliminating phlegm and dampness, rescuing from collapse by restoring Yang, and corresponding classic prescriptions in prevention and treatment of CHF were concluded under the guidance of TCM syndrome differentiation thinking. Meanwhile, research progress on the modern pharmacological effects of these classic prescriptions was systematically discussed, so as to establish a unique treatment system for CHF by classic prescriptions under the guidance of TCM syndrome differentiation theory and provide innovative diagnosis and treatment strategies for clinical CHF.

A Case of Third-degree Atrioventricular Block after Cardiac Radiofrequency Ablation Treated with the Method of Boosting Qi， Warming Yang and Unblocking Collaterals / 中医杂志

Third-degree atrioventricular block is a severe bradyarrhythmia， for which there is no proven effective drugs currently. Permanent pacemaker implantation recommended by the guideline， however， is not suitable for most patients. This paper reported on a case of third-degree atrioventricular block after cardiac radiofrequency ablation who has been treated with the method of boosting qi， warming yang and unblocking collaterals. The TCM syndrome of this case was diagnosed as yang qi depletion and phlegm-stasis blocking the collaterals， for which Baoyuan Decoction and Mahuang Fuzi Xixin Decoction （保元汤合麻黄附子细辛汤） in modification has been used to boost qi， warm yang and raise the sunken， dissolve phlegm， invigorate blood and unblock collaterals. After nearly 7-month treatment， the symptoms such as palpitations， shortness of breath and fatigue were basically cured， and the electrocardiogram returned to the normal.

Staged Treatment of Endometriosis Combined with Infertility Using the Method of Warming Yang and Removing Turbidity / 中医杂志

It is believed that the key pathogenesis of endometriosis combined with infertility is spleen and kidney yang deficiency and binding of dampness and stasis， for which the method of warming yang and removing turbidity is advocated， and self-made Wenyang Huazhuo Formula （温阳化浊方） is recommended with flexibility by stages in accordance with the rules of the changes of yin and yang in the menstrual cycle and the storing and drainage of uterus. Specifically， during the menstruation， it is suggested to warm channels and invigorate blood， drain dampness and remove dampness； during the late menstruation， the method of warming yang and replenishing yin， regulating and supplementing the chong mai （冲脉） and ren mai （任脉）； for inter-menstruation period， it is advised to warm yang and replenish qi， rectify qi and harmonize blood； in terms of premenstrual period， the method of warming and supplementing spleen and kidney， warming uterus and assisting in fertility can be used. Accordingly， Formulas at the menstruation stage， follicular stage， ovulation stage， and luteal stage to warm yang and remove turbidity are recommended in their modifications， respectively.

Effect of Wenyang， Jieyu， and Wenyang Jieyu Prescriptions on Hippocampal Microglia of Mice with Depression-Like Behavior Induced by Secondary LPS Exposure / 中国实验方剂学杂志

Objective:To observe the activation of microglia and the expression of inflammatory factors in hippocampus of mice with depression-like behavior after mother-infant separation （MS） combined with lipopolysaccharide （LPS） stress， and to explore the possible anti-depression mechanisms of Wenyang （WY）， Jieyu （JY）， and Wenyang Jieyu （WYJY） prescriptions from the perspective of warming Yang and relieving depression. Method:Seventy offspring mice were randomly divided into a normal group （<italic>n</italic>=10）， a LPS stress group （<italic>n</italic>=10）， and a modeling group （<italic>n</italic>=50）. After undergoing 8 h·d^-1mother-infant separation during postnatal day 5 （PD₅）–PD₁₄， mice in the modeling group were further divided into the MS + LPS group， WY group， JY group， WYJY group， and fluoxetine （FLU） group， with 10 in each group. The birth date of the offspring mice was recorded as PD₀. The mice in the normal， LPS， and MS + LPS groups were fed a normal diet during PD₂₁–PD₉₀， while those in the other groups were treated with the mixtures of corresponding drugs and feed， followed by seven-day intraperitoneal injection of LPS since PD₉₁ for inducing depression. The depression-like behavior of mice in each group was detected in the open-field， O-maze， and social interaction tests. The protein expression of microglia-specific ionized calcium-binding adaptor molecule 1 （Iba-1） in the hippocampus was assayed by immunohistochemistry， and the mRNA expression of interleukin-1<italic>β </italic>（IL-1<italic>β</italic>）， interleukin-6 （IL-6）， tumor necrosis factor-<italic>α </italic>（TNF-<italic>α</italic>）， Iba-1， and glucocorticoid receptor （GR） by real-time fluorescence quantitative PCR （Real-time PCR）. Result:Compared with the normal group， the LPS group exhibited significantly reduced residence time at the central area within 5 min （<italic>P</italic><0.01） and shortened total exercise distance （<italic>P</italic><0.01）. In the MS + LPS group， the open-arm activity time and the total activity distance decreased significantly （<italic>P</italic><0.01， <italic>P</italic><0.05）， whereas the training， discrimination and exploration time increased significantly （<italic>P</italic><0.01）. The expression of Iba-1 in hippocampal CA1 region of mice in the LPS and MS + LPS groups was remarkably elevated （<italic>P</italic><0.01）. Compared with the LPS group， the MS + LPS group displayed significantly prolonged distance of 5-min exercise （<italic>P</italic><0.05）， increased training， discrimination and exploration time （<italic>P</italic><0.05， <italic>P</italic><0.01）， and up-regulated Iba-1 expression in hippocampal CA1 area （<italic>P</italic><0.01）. As revealed by comparison with the MS + LPS group， both the total 5-min exercise distance （<italic>P</italic><0.01） and the training and discrimination time （<italic>P</italic><0.01， <italic>P</italic><0.05） of mice in each administration group was significantly shortened. The discrimination and exploration time of mice in the JY， WYJY， and FLU groups was significantly reduced （<italic>P</italic><0.01）， and the expression of Iba-1 in hippocampal CA1 region of mice in each administration group was significantly down-regulated （<italic>P</italic><0.01）. Conclusion:The warming Yang and relieving depression method helps to inhibit the occurrence and development of depression due to its efficacy in activating microglia in hippocampus of depression mice and lowering the expression of IL-1<italic>β</italic>， IL-6， and TNF-<italic>α</italic>.

Effect and Mechanism of Qixian Tongluo Prescription on Neural Function Recovery in Patients with Cerebral Infarction / 中国实验方剂学杂志

Objective:To investigate the effect of Qixian Tongluo prescription on neural function recovery in patients with cerebral infarction and its mechanism. Method:A total of 100 inpatients （January to June，2020）with cerebral infarction in the Neurology Department of Wenzhou Hospital of Traditional Chinese Medicine were assigned to an experimental group （<italic>n</italic>=50） and a control group （<italic>n</italic>=50） according to the random number table. Both groups received conventional treatment of western medicine，while the experimental group took additional Qixian Tongluo prescription. Treatment lasted for 12 weeks. The clinical efficacy，National Institutes of Health Stroke Scale （NIHSS） score， the modified Barthel index （MBI），Fugl-Meyer assessment （FMA） score， and levels of brain-derived neurotrophic factor（BDNF），vascular endothelial growth factor（VEGF）， and stromal cell-derived factor-1（SDF-1） in peripheral blood of the two groups before and after treatment were compared. Result:The total response rate in the experimental group was 84.00%（42/50），higher than 66.00%（33/50） in the control group （<italic>Z</italic>=-7.365，<italic>P</italic><0.05）. There was no significant difference in the scores of MBI，FMA， and NIHSS before treatment between the two groups. The MBI and FMA scores of the two groups increased （<italic>P</italic><0.01）， and the NIHSS scores decreased （<italic>P</italic><0.05， <italic>P</italic><0.01）. Compared with the control group after treatment， the experimental group showed increased MBI and FMA scores and decreased NIHSS score （<italic>P</italic><0.05）. There was no significant difference in BDNF level between the two groups before and after treatment. The VEGF and SDF-1 levels in the peripheral blood of the two groups were higher than those before treatment （<italic>P</italic><0.05）， and the experimental group was higher than the control group （<italic>P</italic><0.05）. Conclusion:Qixian Tongluo prescription can effectively improve the clinical efficacy，the quality of life， and the prognosis of patients with cerebral infarction during convalescence. The underlying mechanism is associated with the promotion of the expression of endogenous VEGF and SDF-1 in the peripheral blood to activate the SDF-1/chemokine receptor 4（CXCR4） signaling pathway， induce the recruitment and mobilization of endothelial progenitor cells， and facilitate the angiogenesis and repair of ischemic brain tissues.

Effects of warming yang-dispelling turbidity-dredging collaterals method on renal interstitial fibrosis in rats with chronic renal failure and mechanisms involved / 中草药

Objective To investigate the effects of warming yang-dispelling turbidity-dredging collaterals method on renal interstitial fibrosis in rats with chronic renal failure caused by 5/6 nephrectomy and explore its mechanisms. Methods Eight rats were randomly chosen as sham operation group (Sham), while the remaining 24 rats underwent 5/6 nephrectomy were divided into model group (5/6Nx), warming yang-dispelling turbidity-dredging collaterals method group (WDD) and Niaoduqing group (NDQ), and the rats in each group were administrated with corresponding drugs. After the intervention of 12 weeks, all the rats were sacrificed and the kidneys were then removed. HE, PAS Masson, Picrosirius red staining were conducted to assess kidney pathological changes and renal fibrosis. The protein expression of Collagen was tested by both immunofluorescence and Western blotting, and the mRNA expression was evaluated using qRT-PCR. The expression of protein related with the process of EMT, including α-SMA, E-Cadherin and Vimentin, were tested with immunohistochemistry and Western blotting. Results Compared with the model group, warming yang-dispelling method alleviated kidney pathological injury and renal fibrosis, and significantly down-regulated the protein and mRNA expression of Collagen . Moreover, warming yang-dispelling turbidity-dredging collaterals method regulated the process of EMT by decreasing the high expression of α-SMA and Vimentin, and increasing the low expression of E-Cadherin. Conclusion Warming yang-dispelling turbidity-dredging collaterals method can effectively attenuate renal fibrosis and injury and its underlying mechanisms may be related with its inhibitory effects on Collagen expression and regulation of EMT.

The effect of the yang-warming and fluid retention-resolving method on AngⅡ, TNF-α and IL-6 expression in rats with chronic heart failure / 国际中医中药杂志

Objective To observe the effect of the yang-warming and fluid retention-resolving mothed on neuroendocrine and inflammatory factors in chronic heart failure (CHF) rats. Methods A total of 50 healthy adult male SD rats were randomly divided into blank group (10), CHF group (40). In CHF group, the rat model of chronic heart failure was replicated by intraperitoneal injection of adriamycin (ADR). After the completion of the model making, doppler echocardiography was used to determine the cardiac function of rats. There rats were randomly divided into the model group, the yang-warming and fluid retention-resolving group, the Xuefu-Zhuyu group and the captopril group. After continuous administration for 28 d and 2 h after the last dose, the HE staining was used to observe the pathological morphological changes of myocardium in each group. The levels of AngⅡ, TNF-α and IL-6 in serum were by ELISA. Results Compared with model group, the content of serum AngⅡ(294.33 ± 25.45 pg/ml, 265.86 ± 27.80 pg/ml, 301.87 ± 41.67 pg/ml vs. 615.44 ± 22.31 pg/ml), TNF-α(117.15 ± 11.33 pg/ml, 114.02 ± 11.57 pg/ml, 133.35 ± 34.80 pg/ml vs. 185.72 ± 36.53 pg/ml), IL-6 (23.97 ± 4.77 pg/ml, 27.61 ± 7.01 pg/ml, 28.38 ± 5.78 pg/ml vs. 58.45 ± 6.36 pg/ml) in the yang-warming and fluid retention-resolving group, Xuefu-Zhuyu group and captopril group significantly decreased (P<0.01 or P<0.05). Compared with Xuefu-Zhuyu group, the content of serumAngⅡ (301.87 ± 41.67 pg/ml vs. 265.86 ± 27.80 pg/ml) in the captopril group significantly increased (P<0.05). Conclusions The yang-warming and fluid retention-resolving mothed can reduce early CHF rats through reducing the serum TNF-α, Ang and ILⅡ -6 content, which could be one of the mechanism of action of the treatment of CHF.

Application of the Wenyang Jieyu Xiaocuo Decoction in Treating Yangyu Hanning Acne by Professor WEI Yuegang / 浙江中医药大学学报

[Objective] To summarize Professor WEI Yuegang's experience in treating Yangyu Hanning acne.[Methods]Through collecting medical record from outpatient clinics, Prof. WEI Yuegang's understanding of Yangyu Hanning acne and experience of Yangyu Hanning acne syndrome differentiation and treatment were analyzed and summarized.[Results]Prof. WEI Yuegang believes that the pathogenesis of Yangyu Hanning acne is Yang deficienay and Yang depression, Yang is deficient in the body, and the cold dampness blocks meridians, dysfunction of Shaoyang, Yang deficiency and Yang depression influence each other, Yang cannot supply and distribute, Yin cannot moisten, affter a long time it leads to heat in the upper and cold in the lower, or cold heat complex. In the treatment, we should give priority to warming yang and give consideration to the solution of depression, Prof. WEI Yuegang added and subtract on the basis of the Shenqi Pill and Xiaochaihu Decoction, and formulated Wenyang Jieyu Xiaocuo Decoction for treating the disease. Wenyang Jieyu Xiaocuo Decoction can both warm Yang and dispel cold and resolve Qi stagnation in the provided medical record, so the case achieved good results. [Conclusion]Prof. WEI Yuegang's treatment experience of Yangyu Hanning acne has satisfactory clinical efficacy and is worth to use for reference and promotion.

Effect of Jianxin Decoction on Levels of Inflammatory Cytokines and Cardiac Function of Patients with Chronic Heart Failure / 广州中医药大学学报

Objective To observe the effect of Jianxin Decoction, a recipe with the actions of tonifying Qi and warming Yang, strengthening spleen and removing edema, on the levels of inflammatory cytokines and cardiac function in patients with chronic heart failure (CHF). Methods Sixty-two CHF patients were randomly divided into observation group and control group, 31 cases in each group. The control group was treated with western medicine alone, and the observation group was given Jianxin Decoction combined with western medicine. After treatment for 4 weeks, the levels of serum interleukin -6 (IL-6), interleukin -10 (IL-10), tumor necrosis factor alpha(TNF-α) and brain natriuretic peptide(BNP) were detected by enzyme-linked immunosorbent assay (ELISA). The left ventricular end systolic diameter (LVESD), left ventricular end diastolic diameter(LVEDD), left ventricular ejection fraction(LVEF), cardiac output(CO) and ratio of mitral peak velocity of early filling to mitral late diastolic peak velocity(E/A) were determined with echocardiography. The effect on traditional Chinese medicine (TCM) syndromes, effect on cardiac function grading, and adverse reaction in the two groups were also observed. Results (1) After treatment, the effective rate on TCM syndromes and improvement of cardiac function in the observation group were superior to those in the control group, the difference being significant (P<0.05). (2) Levels of serum IL-6, IL-10, TNF-αand BNP of the two groups after treatment were decreased as compared with those before treatment(P < 0.05), and the decrease in the observation group was superior to that in the control group (P<0.05). (3)After treatment, LVSED and LVEDD of the two groups were much decreased, and LVEF, E/A and CO were markedly increased (P < 0.05 compared with those before treatment). The improvement of LVSED, LVEDD, LVEF, E/A and CO in observation group was significantly superior to that in the control group (P < 0.05). (4) During the treatment, the incidence of adverse reaction showed no significant differences between the two groups (P > 0.05). Conclusion Jianxin Decoction combined with western medicine can effectively reduce the levels of inflammatory cytokines and BNP, so as to relieve the clinical symptoms and improve cardiac function grading in patients with CHF. The combination has better effect and is safer than western medicine alone for CHF.

Effects of warming yang and invigorating qi prescription on renin-angiotensin-aldosterone system in rats with heart failure after myocardial infarction

This study investigated the effects of warming yang and invigorating qi prescription on renin-angiotensin-aldosterone system (RAAS) in rats with heart failure after myocardial infarction. 126 rats were randomly divided into model group, sham operation, warming yang, invigorating qi, warming yang+invigorating qi, digoxin and captopril group for respective treatment. After intervention for 6, 8 and 10 weeks, the left ventricular ejection fraction (LVEF) was calculated, and the plasma renin, angiotensin II and aldosterone levels were measured. Results showed that, after 6, 8 and 10 weeks, LVEF in warming yang, invigorating qi, warming yang+invigorating qi and captopril group was significantly higher than model group (P < 0.05), and the plasma renin, angiotensin II and aldosterone levels in warming yang, invigorating qi, warming yang+invigorating qi and captopril groups were significantly lower than model group (P < 0.05). Renin angiotensin II and aldosterone levels in invigorating qi, warming yang+invigorating qi and captopril groups after 10 weeks was significantly lower than after 6 weeks (P < 0.05); aldosterone level in captopril groups after 10 weeks was significantly lower than after 6 weeks (P < 0.05). Warming yang and invigorating qi prescription can improve LVEF in rats with heart failure after myocardial infarction, which may be related with the inhibition of RAAS activation.(AU)

Efficacy of Benefiting Qi for Warming Yang and Activating Blood Circulation with Western Medicine for Inducing Diuresis in the Patients with Chronic Heart Failure and its Effect on the Expression of Circulating miRNAs / 中国药师

Objective:To observe the efficacy of benefiting Qi for warming Yang and activating blood circulation for inducing diu-resis method in the treatment of patients with chronic heart failure( CHF) and its effect on the expression of circulating miRNAs ( miR-423-5p, miR-210-3p). Methods:Totally 80 CHF patients were randomly divided into two groups,the control group with 40 patients was treated with the conventional anti-heart failure therapy, and the treatment group with 40 patients was treated with benefiting Qi for warming Yang and activating blood circulation for inducing diuresis decoction on the basis of the control group, and the treatment course was 2 weeks. The clinical efficacy and syndrome integral of traditional Chinese medicine ( TCM) were evaluated. Left ventricular ejec-tion fraction ( LVEF) and left ventricular end diastolic diameter( LVEDD) were measured by echocardiography;the plasma expression levels of circulating miRNAs(miR-423-5p, miR-210-3p) were measured by real-time PCR in the two groups before and after the treat-ment. The adverse drug reactions were observed as well. Results:The total clinical effective rate of the treatment group was significant-ly higher than that of the control group (87. 50% vs 65. 00%, P <0. 05); the syndrome integral of TCM, indices of LVEF and LVEDD and the expression of circulating miRNAs were improved in both groups(P<0. 05);the total clinical effective rate, syndrome integral of TCM, indices of LVEF and LVEDD and the expression of circulating miR-423-5p of the treatment group were better than those of the control group(P<0. 05); there was no significant difference in adverse drug reactions between the groups (P>0. 05). Conclusion:The benefiting Qi for warming Yang and activating blood circulation for inducing diuresis method can improve the clinical effects in the treatment of CHF, and improve the expression of circulating miRNAs.

The clinical curative effect of warming yang for diuresis of traditional Chinese medicine combined with blood purification for treatment of acute renocardiac syndrome / 中国中西医结合急救杂志

Objective To explore the effect of warming yang for diuresis method combined with blood purification on the clinical therapeutic effect of patients with acute renocardiac syndrome (ARCS).Methods Sixty ARCS patients admitted to the Department of Intensive Care Unit (ICU) of Affiliated Hospital of Tianjin Institute of Chinese Medicine from May 2013 to October 2016 were enrolled, they were divided into an observation group and a control group by random number table, 30 cases in each group, one case died of multiple organ failure due to aggravation of infection, thus the actual observation number was 29 patients in the control group. The continuous vein-vein hemofiltration (CVVH) was applied in the two groups, 6-13 hours each time, and according to the disease situation, the continuous ultrafiltration time was determined, generally about 3-7 days; the observation group was additionally treated with warming yang for diuresis oral herbal decoction, 1 dose daily, 2 times a day, once 100 mL orally taken. The changes of cardiac and renal function indexes, traditional Chinese medicine (TCM) syndrome score and clinical efficacy were observed before and after treatment in the two groups.Results After treatment, the heart and renal function indexes were significantly improved, serum creatinine (SCr), urea nitrogen (BUN), N-terminal pro-brain nitric peptide (NT-proBNP) were all significantly lower than those before treatment in both groups, glomerular filtration rate (GFR), urine output, left ventricular ejection fraction (LVEF), cardiac output index (CI) were all significantly higher than those before treatment in both group; 7 days after treatment, BUN was significantly lower in observation group than that in control group (mmol/L:10.38±1.02 vs. 13.68±2.67), GFR, urine output, CI were significantly higher in observation group than those in control group [GFR (mL/min): 62.02±4.47 vs. 52.95±1. 92, urine output (mL/24 h): 875.41±44.26 vs. 537.82±79.65, CI (L·min-1·m-2): 3.12±0.51 vs. 3.07±0.17, allP < 0.05]; the effective rate of TCM syndrome score and clinical efficacy were significantly higher in observation group than those in control group [total effective rate of TCM syndrome score: 80.0% (24/30) vs. 55.17% (16/29), total effective rate: 93.33% (28/30) vs. 72.41% (21/29), both P < 0.05].Conclusions The warming yang for diuresis TCM decoction intake combined with CVVH can improve the cardiac and renal functions and clinical symptoms, and increase urine output for patients with ARCS.

"Based on ""pulmonary gangrene"" warming yang and supporting, promoting blood circulation and Huoxuegongtan method for treatment of pulmonary alveolar proteinosis" / 中国中西医结合急救杂志

Pulmonary alveolar proteinosis (PAP) is a rare disease clinically, and at present no western medicine can effectively cure the disease. After the analysis of literatures related to Traditional Chinese Medicine (TCM) for treatment of the disease in the China National Knowledge Internet Database, it was discovered that the syndrome differentiation showed the disease closely associated with phlegm, blood stasis and yin deficiency. In this study, from view point of pulmonary gangrene TCM theory and treatment combined with PAP pathophysiological characteristics, it is emphasized that phlegm accumulation, blood stasis and yang deficiency are the key pathological manifestations of PAP, thus Yanghe decoction, Tounong powder, Kongxian pellet were used for treatment of one such case, the rational thinking in diagnosis and treatment of this patient being consistent with the idea learning western medicine for China use.

"Therapeutic Observations of""Wen Yang Zhu Shui Formulae""Combined with Moxa Salt Packets in the Treatment of Malignant Ascites" / 浙江中医药大学学报

[Objective] To observe the clinical effect ofWen Yang Zhu Shui Formulaecombined with moxa salt packets in the treatment of malignant ascites. [Methods] 61 patients were randomly divided into an observation group(n=31) and a control group(n=30). The patients in the control group were treated with western medicine, while patients in the observation group, based on the treatment of control group, were treated with Wen Yang Zhu Shui Formulaecombined with moxa salt packets for one time a day for two weeks. The effective rates for malignant ascites and Karnofsky performance score(KPS) were compared in the two groups. [Results] The effective rate for malignant ascites in the observation group(80.64%) was higher than that in the control group (53.33%), P<0.05. The observation group showed significantly higher score of KPS than patients of the control group( P<0.05). [Conclusion]Wen Yang Zhu Shui Formulaecombined with moxa salt packets can effectively reduce the malignant ascites.

Effect of “Warming Yang and Nourishing Qi”Acupuncture on AChR mRNA expression in neuromuscular junction of rats with experimental autoimmune myasthenis gravis / 吉林大学学报(医学版)

Objective:To observe the effect of “Warming Yang and Nourishing Qi” Acupuncture on the acetylcholine receptor (AchR)mRNA expression in neuromuscular junction (NMJ)of the rats with experimental autoimmune myasthenis gravis (EAMG), and to analyze the mechanism of acupuncture treatment for EAMG. Methods:AchRα1 129-145 peptide fragments were used to immune the 60 female Lewis rats to establish the EAMG rat models. 30 EAMG rats were selected randomly and divided into model control group,acupuncture treatment group and drug control group (n = 10).Another 10 SPF- “grade three”female Lewis rats,which were raised together with EAMG rats,were used as blank control group. The rats in model control group and blank control group had no treatment.The rats in acupuncture treatment group were treated with “Warming Yang and Nourishing Qi”Acupuncture.The rats in drug control group were treated with pyridostigmine bromide by gavage. The tissue samples in NMJ of gastrocnemius of the rats were collected,and the expression levels of AChR mRNA were detected with Real-time PCR method; the differences among various groups were analyzed quantitatively. Results:Compared with blank control group,the expression levels ofγ-AChR mRNA andε-AChR mRNA in NMJ of the rats in acupuncture treatment group drug control group and model control group were significantly increased (P <0.01).Compared with model control group,theγ-AChR mRNA andε-AChR mRNA in NMJ of the rats in drug acupuncture treatment group and control group were significantly increased (P < 0.01 ). Conclusion:“Warming Yang and Nourishing Qi”Acupuncture can effectively improve the expression level of AchR mRNA and it is an effective treatment method for myasthenia gravis.

Effect of“Warming Yang and Nourishing Qi”Acupuncture on acetylcholine receptor protein expression in neuromuscular j unction of rats with EAMG / 吉林大学学报(医学版)

Objective:To study the influence of “Warming Yang and Nourishing Qi”Acupuncture in the expression levels of acetylcholine receptor protein (AchR)in gastrocnemius neuromuscular junction (NMJ)of the rats with experimental autoimmune myasthenia gravis (EAMG),and to clarify the mechanism of “Warming Yang and Nourishing Qi”Acupucture in the treatment myasthenia gravis (MG).Methods:The female Lewis rats were used to establish the EAMG rat models immuned with AchRα1 129-145 polypeptide fragments.30 EAMG rats were selected randomly and divided into model control group, drug control group, and acupuncture treatment group (n=10),and another 10 rats purchased at the same period were used as blank control group.The rats in drug control group were treated with 1 8.5 mg · kg-1 · d-1 pyridostigmine by gavage. And the rats in acupuncture treatment group were treated with irritating by“Warming Yang and Nourishing Qi”Acupuncture.The rats in blank control group and model control group were not treated .The Lennon symptom scores of the rats in various groups were recorded.After treatment,the tissues of gastrocnemius NMJ of the rats were taken,and the AchR protein expression levels in NMJ of the rats in various groups were detected by Western blotting method. Results:Compared with blank control group,after successfully modelling,the Lennon symptom scores of the rats in model control group, drug control group and acupuncture treatment group before treatment were significantly increased (P<0.01).Compared with model control group,the Lennon symptom scores of the rats in drug control group and acupuncture treatment group after two treatment course were significantly increased (P<0.01 ). Compared with blank control group, the AchR protein expression level in gastronemus NMJ of the rats in model control group was significantly decreased (P<0.01). Compared with model control group, the AchR protein expression levels in gastronemus NMJ of the rats in acupuncture treatment group and drug control group after treatment were significantly increased (P<0.01).Conclusion:“Warming Yang and Nourishing Qi”Acupuncture has good therapeutic effect on the EAMG through increasing the NMJ AchR protein expression level of the EAMG rats.

Clinical Observation of Zuo’s Yang-warming Acupuncture Therapy for Treatment of Allergic Rhinitis / 广州中医药大学学报

Objective To observe the clinical efficacy of yang-warming acupuncture therapy established by Zuo Changbo for the treatment of allergic rhinitis. Methods Sixty patients with allergic rhinitis were randomly divided into observation group and control group, 30 cases in each group. The observation group was given Zuo’s yang-warming acupuncture therapy and the control group was given conventional acupuncture therapy. Acupuncture was performed once every other day, and 10 times constituted one course of treatment. After treatment, the therapeutic effect was evaluated, and the symptom scores of nasal itching, stuffy nose, sneezing and nasal discharge as well as their visual analog scale ( VAS) scores were observed. Results ( 1) Total effective rate of the observation group was 93.3%, and that of the control group was 63.3%, there was significant difference between the two groups ( P<0.01). ( 2) After treatment, the scores of the four symptoms were decreased obviously ( P<0.05 or P<0.01) , and the decrease in the observation group was superior to that in the control group ( P<0.01) . ( 3) After treatment, the VAS scores in both groups were decreased ( P<0.01) , and the decrease was more obvious in the observation group (P<0.01). Conclusion The curative effect of yang-warming acupuncture therapy on allergic rhinitis is better than that of the routine acupuncture group.

Effect ofIllicium verum Water Extract on Excess-cold Syndrome in Rats / 世界科学技术-中医药现代化

This study was aimed to observe the effect ofIllicium verum water extract on excess-cold syndrome in rats. The intragastric administration of the decoction of gypsum, gentian, phellodendron andrhizoma anemarrhenae (2:1.2:1:1.5) was used to establish the excess-cold rat model. Effects ofI. verum water extract on general body signs of excess-cold syndrome rats were observed. The detection was made on the contents of LD, PA, TG, TSH, T4 in the serum and LD, PA, glycogen in liver tissues. The vitality of the LDH, SDH and ATPas was also detected. The results showed thatI. verum water extract can improve the general body signs of excess-cold syndrome rats. It reduced the content of TG; increased the content of PA, T4, TSH; reduced the content of LD, glycogen, and the vitality of LDH;increased the vitality of LDH, Ca2+-Mg2+-ATPase in liver tissues. It was concluded thatI. verum water extract can improve some general body signs of excess-cold syndrome rats, improve the substance and energy metabolism, and regulate the thyroid axis function, in order to achieve the warmingyang for dispelling cold effect.

Clinical Application of Qianyang Fengsui Pill / 浙江中医药大学学报

[Objective] To sum up the clinical efficiency of Qianyang Fengsui Pil (suppress Yang and seal marrow)Pil (hereafter cal ed Pil ). [Method] By introducing medicine composition, functions and indications, it enumerates 3 cases to analyze the clinical application of the Pil . [Result] The Pil is the mostly recommended by vulcans, with function of inducing Qi to kidney and fire to the root.[Conclusion] The Pil has good clinical cure effect on the diseases of fire not returning to the origin and deficient Yang come-up, worth wide application.

Discussing on the therapy of warming yang in the treatment of tumor / 国际中医中药杂志

The therapy of removing beat and toxic,strengthening the body to cultivate the fundamental is the mainstream of treating cancer in traditional Chinese medicine up to now,and the therapy of warming yang has been rarely involved.While the pathogenesis of tumor is spleen and kidney yang deficiency in root,and phlegm,stasis,and poison entangled with each other at the secondary position; the main therapeutical principle of tumor is simultaneous application of purging-tonifying therapy while focusing on protecting yang.The author believes that the treatment of cancer should pay attention to the whole concept,especially warming yang for patients with advanced tumors.The therapy of warming yang is an effective way to get rid of phlegm and blood stasis.Patients after radiotherapy and chemotherapy should be paid more attention to warming yang.