Analysis of the correlation between smoking, white blood cell counts and serum bilirubin levels in male radiation workers / 公共卫生与预防医学

Objective To investigate the correlation between smoking, white blood cell counts, and serum bilirubin levels in male radiation workers, and to explore the role of systemic inflammatory response in the changes of serum bilirubin level induced by smoking. Methods Occupational health examination data of 1 320 male radiation workers in a medical institution was collected. Linear regression analysis method was used to analyze the correlations between smoking and white blood cell counts, between smoking and serum bilirubin levels, and between white blood cell counts and serum bilirubin levels. Results There was a significant positive correlation between smoking and white blood cell counts (P 0.05). By dividing white blood cell counts into two groups, an inverse correlation was found between smoking and serum bilirubin levels in the high white blood cell count group (P < 0.05). Conclusion Serum bilirubin may be an effective indicator of early health damage caused by smoking in male radiation workers. Smoking may induce inflammatory reaction, thus deplete serum bilirubin and cause its levels to drop.

Analysis of hematologic parameters of donors, patients, and granulocyte concentrates to predict successful granulocyte transfusion

BACKGROUND: Granulocyte transfusion (GTx) is performed as a supportive therapy in severe neutropenic patients caused by various conditions. The study aimed to analyze the hematologic parameters of donors, patients, and granulocyte concentrates to predict successful GTx. METHODS: This study was performed in 281 donors, with their granulocyte concentrates being collected through apheresis, and in 54 severe neutropenic patients who had various hematologic diseases. Complete blood cell counts of donors pre- and post-apheresis, granulocyte concentrates, and patients pre- and post-GTx were analyzed. Patients were divided into two groups according to survival at discharge (Group S, survival; Group D, dead) to compare various factors including age, infection status, pre- and post-GTx total white blood cell counts (TWBCC) and absolute neutrophil counts (ANC), total number of GTx, infused TWBCC and ANC per weight, and use of G-CSF during therapy. RESULTS: Overall data of patients showed that both TWBCC and ANC were significantly increased after GTx (median values at pre-GTx, TWBCC=0.40×109/L, ANC=0.14×109/L; post-GTx, TWBCC=0.57×109/L, ANC=0.29×109/L, both P<0.0001). After GTx, Group S (N=25) showed significantly higher TWBCC and ANC than Group D (N=29) (P=0.01 and P=0.04, respectively). Using different cutoff levels, post-GTx TWBCC greater than 0.5×109/L showed statistically significant difference between the two groups (P<0.01). None of the other factors showed statistically significant differences. CONCLUSION: The TWBCC and ANC after GTx were significant factors to predict patients' outcome. Therefore, follow-up of those two parameters may be helpful to select or consider other therapeutic modalities including additional GTx.

Effect of Carica papaya (L) Leaves on Haematological Parameters in Ccl4-induced Wistar Albino Rats.

Aims: To investigate the effect of Carica papaya leaves on some haematological parameters (PCV, RBC, Hb, WBC and differential blood counts) were investigated. Place and Duration of Study: Department of Medical Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Niger Delta University, Wilberforce Island, Amassoma, Bayelsa-State, Nigeria, between. Methods: Thirty male albino rats were randomly allotted to five groups of six rats per group. Haemoglobin (Hb) was determined spectrophotometrically by the cyanomethaemoglobin method, Red blood cells (RBC), was estimated by haemocytometer, using adopted standard procedure. Group1 (negative control) were fed with 100% rat feed. Groups 2- 4 were pretreated with 10, 30 and 50% C. papaya L respectively, while Group 5 (normal control) received 100% rat feed. Rats in groups (1-4), were injected with CCl4 (0.5 ml/kg body weight in 0.5 ml olive oil) on the 29th day while rats in group 5 were not administered with CCl4 (normal control). Results: The results were statistically analyzed using one-way analysis of variance (ANOVA). There were significant increases (p≤ 0.05) in the levels of Hb, PCV, RBC, lymphocytes and decreased WBC and neutrophils in rats in group 5 (normal control) as against the negative control (group 1). Rats groups pretreated with 10, 30 and 50% Carica papaya (groups 2, 3 and 4 respectively), showed significant increased (p≤ 0.05) PCV, RBC and Hb levels, when compared with untreated rats (group 1). Rats that were administered with CCl4 only (negative control), showed significant increases (p≤ 0.05) in the levels of WBC and neutrophils. However, incorporation of 10, 30 and 50% Carica papaya in groups 2, 3 and 4 respectively, significantly decreased the levels of WBC and neutrophils, when compared with rats in untreated group 1. Monocytes levels significantly increased (p≤ 0.05) in rats pre-treated with 30% and 50% Carica papaya (groups 3 and 4 respectively). While, there was zero level of basophils in all the groups. Conclusion: Carica papaya L, may therefore possess and confer erythropoietic properties on rats pretreated groups as evident in the increased levels of Hb, PCV, RBC and lymphocytes.

Factors Related to the Thrombocytosis in Children with Iron Deficiency Anemia / 대한소아혈액종양학회지

PURPOSE: We investigated to identify factors related to thrombocytosis and clinical data for thrombopoiesis in children with iron deficiency anemia (IDA). METHODS: We retrospectively analyzed clinical and laboratory data for 85 children admitted for acute infection or inflammation. Seventy patients of 85 children were diagnosed as IDA. The others were clinically suspected as IDA but they were not diagnosed. We divided three groups: group 1 included severe anemia below hemoglobin (Hb) 8.0 g/dL, group 2 mild to moderate anemia (Hb: 8.0~10.0 g/dL), and group 3 (control) were clinically suspected but without IDA. RESULTS: There are no differences among groups except age at diagnosis. The age at diagnosis in group 1 are higher than other groups. In control group, there are not any factors correlated with thrombocytosis. In group 1, the white blood cell and lymphocyte counts are significantly related to the platelet counts. However, serum iron level is only correlated with platelets in group 2. In multiple regression analysis, we found significantly correlation between white blood cell counts and serum iron level and thrombocytosis in IDA including group 1 and 2. CONCLUSION: We suggest that white blood cell counts and serum iron level in IDA may be related with increased platelet counts, as a reactive thrombocytosis. We need further study for correlation between acute phase reactants and thrombocytosis in IDA.

Clinical Analysis of Patients with Acute Appendicitis Operated on during

PURPOSE: During the night time decision making on patients suspicious of appendicitis is often difficult because diagnosis and timing for operation are frequently delayed. Therefore, we analyzed above cases and solution is suggested. METHODS: This retrospective study included 360 patients who underwent laparotomies for suspected appendicitis at Hanil Hospital during one year, from March 1998 to Feb. 1999. They were divided into two groups according to presenting time to physician (Day time: 6 a.m. to 6 p.m., Night time: 6 p.m. to 6 a.m.). Sex & age distribution, time of presentation to physician, duration of symptoms, symptoms & physical findings, white blood cell counts, interval from presentation to operation, hospital stay, and pathologic diagnosis were compared. RESULTS: There were no significant differences in sex & age distribution, duration of symptoms, symptoms & physical findings, white blood cell counts, pathologic diagnosis between the two groups. However, during the night time, the interval from presentation to operation was longer than that of the day time (9.15 hours versus 4.83 hours, p<0.001), the rate of delayed appendectomy during the night was 58.0%, the rate of negative laparotomy increased when appendectomy was delayed for more than 12 hours compared with less than 12 hours (28.1% vs 11.7%, p<0.01), and in the cases with perforated appendicitis, delayed appendectomy for more than 12 hours had longer hospital stay compared with less than 12 hours (12 days vs 9.44 days, p<0.01). Factors causing delayed appendectomy were related to the physician (42.5%), lack of anesthetic & nursing supports (19.5%), failure to structure the operation team (20.7%), and patient itself (17.3%). When white blood cell counts were rechecked in the next morning, levels above 10,000 cells/mm3 were highly associated with appendicitis in contrast to that below 10,000 cells/mm3 (91.7% vs 43.5%, p<0.002).