Effect of aging on tear strength and cytotoxicity of soft denture lining materials; in vitro

PURPOSE: The aim of this in vitro study was to evaluate the effect of aging on the tear strength and cytotoxicity of four soft denture lining materials. MATERIALS AND METHODS: Four commonly used soft denture lining materials, (Coe-Comfort(TM) GC America Inc., Alsip, IL, USA; Coe-Soft(TM) GC America Inc., Alsip, IL, USA; Visco-gel Dentsply Caulk Milford, DE, USA; and Sofreliner Tough M Tokuyama Dental Corporation Tokyo, Japan) were selected. Sixty trouser-leg designed specimens per lining material were fabricated using a stainless steel mold for tear strength testing. The specimens were divided into non-thermocycling and 1000-, and 3000- thermocycling groups. For the cytotoxicity test, twenty-four disk shaped specimens per material were fabricated using a stainless steel mold. The specimens were soaked in normal saline solution for 24 h, 48 h and 72 h. Cytotoxicity was measured by XTT assay in L929 mouse fibroblasts. Data were analyzed by two way analysis of variance and Dunnett's test (P<.05). RESULTS: Before thermocycling, Sofreliner Tough M (10.36 +/- 1.00 N) had the highest tear strength value while Coe-Comfort(TM) (0.46 +/- 0.10 N) had the lowest. After 3000 cycles, Sofreliner Tough M (9.65 +/- 1.66 N) presented the highest value and Coe-Comfort(TM) (0.42 +/- 0.08 N) the lowest. Sofreliner Tough M, in all incubation periods was the least toxic with significant differences compared to all other materials (P<.05). Coe-Comfort(TM), Coe-Soft(TM), and Sofreliner Tough M did not show any significant differences within their material group for all incubation periods. CONCLUSION: This in vitro study revealed that aging can affect both the tear strength and cytotoxicity of soft denture materials depending on the composition.

Two colorimetric assays verify that calcium sulfate promotes proliferating activity of human gingival fibroblasts / 대한치과보철학회지

STATEMENT OF PROBLEM: The role of calcium sulfate in stimulating the growth of gingival soft tissue has been reported in few studies. Such a unique property of calcium sulfate could serve as a trouble-solving broker in compensating for the lack of soft tissues in various oral surgeries. PURPOSE: The purpose of this study was to compare the proliferating activities of human gingival fibroblasts seeded on various bone graft barrier materials of calcium sulfate, collagen, and polytetrafluorethylene (PTFE). MATERIAL AND METHODS: Two calcium sulfates (CAPSET(R) and CalForma(R), Lifecore Biomedical Inc., St. Paul, Minnesota, USA), a resorbable natural collagen (Bio-Gide(R), Geistlich Pharma Ag., Wolhusen, Switzerland), and a non-resorbable PTFE (TefGen-FD(R), Lifecore Biomedical Inc., St. Paul, Minnesota, USA) served as the human gingival fibroblasts'substrates and comprised the four experimental groups, whereas the untreated floors of culture plastics were used in the control group, in this study. Cells were trypsinized, seeded, and incubated for 48 h. The proliferating activities of fibroblasts were determined by XTT and SRB assay and absorbance (optical density, OD) was measured. One-way ANOVA was used to analyze the differences in the mean OD values between the groups of CAPSET, CalForma, Bio-Gide, TefGen, and the control (p<0.05). RESULTS: From the XTT assay, the mean OD value of the control group, the highest, was significantly greater than that of any of the four experimental groups followed by CalForma, CAPSET, TefGen, and Bio-Gide. Further, the mean OD value of CalForma, was significantly greater compared to that of Bio-Gide. From the SRB assay, Calforma showed the highest mean OD value, which was significantly greater than that of any other groups, followed by the control, CAPSET, Bio-Gide, and TefGen. The mean OD values of both the control and CAPSET were significantly greater compared to that of TefGen (p<0.05). CONCLUSION: Assessment of the viability and proliferation of cultured fibroblasts seeded and incubated for 48 h on various barrier-material substrates using XTT and SRB assay showed that calcium sulfate CalForma(R) promotes the proliferating activity of human gingival fibroblasts.