Résumé
Objective To investigate up-regulation of lncRNA-AK058003 expression in SK-Hep1 hepatocellular carcinoma cells and its effect on the growth of human hepatocellular carcinoma xenograft tumor in nude mice.Methods The recombinant plasmid of lncRNA-AK058003 and empty plasmid were transferred into SK-Hep1 human hepatocellular carcinoma cell line by the method of slow virus transfection.The overexpression of lncRNA-AK058003 cell line was screened by neomycin.Overexpression of lncRNA-AK058003 in SK-Hep1 hepatocarcinoma cells was detected by quantitative PCR.SK-Hep1 hepatocellular carcinoma cells stably overexpressing lncRNA-AK058003 and SK-Hep1 hepatocellular carcinoma cells containing empty plasmids were injected subcutaneously into armpit of nude mice.The long and short diameters of the transplanted tumor were measured regularly to calculate the volume of the xenograft tumor.After 35 days of feeding we measure the weight of the xenograft tumor.Results The recombinant plasmid of lncRNA-AK058003 was successfully constructed and stably over expressed in hepatocellular carcinoma cells.The experimental results of subcutaneous tumor bearing nude mice showed that the volume(308.4 ± 439.4mm3),weight (0.464 ± 0.518g)and growth rate of the xenograft tumor in the over expression group were lower than that of the empty plasmid group(1410.0 ± 973.0mm3,1.363 ± 0.856g,P ＜ 0.05).Conclusion LncRNA-AK058003 has inhibitory effect on the proliferation of human hepatocellular carcinoma xenograft tumor in nude miee.
Résumé
Objective To investigate up-regulation of lncRNA-AK058003 expression in SK-Hep1 hepatocellular carcinoma cells and its effect on the growth of human hepatocellular carcinoma xenograft tumor in nude mice.Methods The recombinant plasmid of lncRNA-AK058003 and empty plasmid were transferred into SK-Hep1 human hepatocellular carcinoma cell line by the method of slow virus transfection.The overexpression of lncRNA-AK058003 cell line was screened by neomycin.Overexpression of lncRNA-AK058003 in SK-Hep1 hepatocarcinoma cells was detected by quantitative PCR.SK-Hep1 hepatocellular carcinoma cells stably overexpressing lncRNA-AK058003 and SK-Hep1 hepatocellular carcinoma cells containing empty plasmids were injected subcutaneously into armpit of nude mice.The long and short diameters of the transplanted tumor were measured regularly to calculate the volume of the xenograft tumor.After 35 days of feeding we measure the weight of the xenograft tumor.Results The recombinant plasmid of lncRNA-AK058003 was successfully constructed and stably over expressed in hepatocellular carcinoma cells.The experimental results of subcutaneous tumor bearing nude mice showed that the volume(308.4 ± 439.4mm3),weight (0.464 ± 0.518g)and growth rate of the xenograft tumor in the over expression group were lower than that of the empty plasmid group(1410.0 ± 973.0mm3,1.363 ± 0.856g,P ＜ 0.05).Conclusion LncRNA-AK058003 has inhibitory effect on the proliferation of human hepatocellular carcinoma xenograft tumor in nude miee.