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Objective To investigate the effects of Yanshu injection for the chemotherapy, toxicity- reducing and immunization in mice implanted tumor. Methods Bulid up the mice models with S180 solid tumor transfered by ascites tumor, and observe the effect on tumor weight, blood WBC and bone marrow nucleated cells when combined use Yanshu injection and cyclophospamide (Cy). Determine OD value of plague forming cell by spectrophotometry, measure T-lymphocyte transformation by 3H-TDR incorporation, and survey natural killer cell (NKC) activity by colorimetric method, all these can help to study the role of Yanshu injection on the immune effect in the mice models. Results Yanshu injection can strengthen antitumor activity of Cy, antagonize the inhibition of Cy to WBC and bone marrow nucleated cell, improve OD value of plague forming cell, promote T-lymphocyte transformation, also can enhance NKC activity and killing rate. Conclusion Yanshu injection can increase the chemotherapeutic effects, reduce toxin, and enhance immune function of mice implanted tumor.
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Objective To discuss the clinical mechanism of Yanshu injection by observing its function to the tumor cells SGC-7901,HepG2 and BEL-7402 in vitro.Method The kill rate by MTT,flow cytometry were used to detect the change of cell cycle and apoptosis rate,and the expression of bcl-2,CD44V6 and nm23 of HepG2,SGC-7901 and BEL-7402 were observed.Results In vitro,to HepG2,SGC-7901 and BEL-7402,the living rate after using Yanshu injection was 72 %,40 % and 50 %.Compared with the control,the cell quantity in S phase decreased,and the apoptosis rate was higher,the expression of CD44V6 and nm23 changed a lot.Conclusion To the tumor cells,HepG2,SGC-7901 and BEL-7402,Yanshu injection can kill them,arrest them in G2 phase,block the conversion to M phase,accelerate cell apoptosis which has no correlation with the expression of bcl-2,and it still can restrain tumor metastasis.
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0.05).The median survival time was 32 weeks in group A compared to 27 weeks in group B(P