Résumé
Objective To investigate the effect of Yishen Jianpi Yangxue Tongli fang (YSJP) on PCOS rats and approach the action mechanism of the recipe. Methods The animal model of levonorgestrel’s PCOS rat was established to observe the pregnancy rate and examinational results of light and electron-microscope. Results YSJP could promote pregnancy. The examinational results of light and electron-microscope showed that YSJP could recover the abnormal structure of organelle of the ovary. Conclusion YSJP had the effect of promoting pregnancy, recovering the injured organelle.
Résumé
Objective To investigate the effects of Yishen dianpi Yangxue Tongli Fang (YSJP) on IGF-1 mRNA expression in the ovary of PCOS rat, and study its mechanism on the molecular level. Methods Seventy two female SD rats were randomly divided into normal control group, model group, clomifene group and low-, mid-, high-dose Chinese medicine group. The animal model established by capsulae levonorgestreli silasticus was used to observe the IGF-1 mRNA expression in ovary by hybridization in situ. Results IGF-1 mRNA expression of PCOS model group was higher than the normal control group obviously. The semi-quantitative image analysis showed that TCM treatment could decrease IGF-1 mRNA expression. Conclusion Yishen Jianpi Yangnue Tongli Fang could decrease the IGF-1 mRNA expression in ovary and improve the insulin resistance of PCOS rats.