RÉSUMÉ
This study was aimed to observe the effect of Yue-Ju Bao-He (YJBH) pill and simvastatin on the reversal of myocardial fibrosis induced by atherosclerosis (AS).ApoE-/-mice were fed on high fat diet to establish models of atherosclerotic plaque and myocardial fibrosis.YJBH pill and simvastatin were used alone or in combination as interventions.Control groups of high fat diet as well as normal diet were observed.Serum lipid and vascular angiotensin Ⅱ (Ang Ⅱ),matrix metalloproteinase-1 (MMP-1) were measured by Elisa.Quantitative analysis of collagen volume fraction (CVF) was conducted by myocardial tissue Masson staining.High-throughput transcriptome sequencing analysis was also adapted to detect differentially expressed genes in myocardial tissues of each group.The results showed that compared with single usage,YJBH pill combined with simvastatin can effectively reduce the total cholesterol (TC),triglyceride (TG),low density lipoprotein (LDL-C) and Ang Ⅱ,increase high density lipoprotein (HDL-C),MMP-1,while Masson staining CVF value was also significantly decreased.Compared with the control group of normal diet,the analysis results from high-throughput transcriptome sequencing analysis showed that difference of gene expression of YJBH pill combined with simvastatin group was mainly in fatty acid metabolism,extend and degradation related pathways,and compared with the model group,the difference of gene expression was mainly in primary immunodeficiency and intestinal immune network IgA production pathways.It was concluded that the combined therapy of YJBH pill and simvastatin can effectively reverse the AS induced myocardial fibrosis.Its mechanism is related to the regulation of immune inflammatory reaction.