Résumé
High selectivity provided by biomolecules such as antibodies and enzymes has been exploited during the last two decades for development of biosensors. Of particular importance are efficient immobilization methods for biomolecules in order to preserve their biological activities. In this study, we have evaluated immobilization strategies for an anti-DNA antibody on a self-assembled monolayer of omega-functionalized thiols. The antibody was immobilized via peptide bond formation between the primary amines in the antibody and the carboxyl groups on the self-assembled monolayer. The peptide bond coupling was achieved by activating COOH groups on the surface through N-Hydroxysuccimide (NHS)-ester formation, followed by acylation of NH2 group in the antibody. DNA binding activity of the immobilized antibody was examined by counting beta emission from 35S-labeled DNA.
Sujets)
Anticorps antinucléaires , ADN/immunologie , ADN/analyse , Protéines de liaison à l'ADN/composition chimique , Or , Membrane artificielle , Réaction de polymérisation en chaîne , Polyvinyles/composition chimique , Dosage radioimmunologique/méthodes , Acide lipoïque/composition chimiqueRésumé
One hundred and fifty-seven cases of Graves' disease were divided into three groups according to their clinical features and thyroid functions: Group A, untreated; group B, treated but uncontrolled or relapsed; group C, treated and remitted. The positive rates of TSH receptor antibody (TRAb), anti-tnyroglobulin antibody (TgAb), anti-microsomal antibody (TmAb), anti-DNA antibody and anti-nuclear antibody (ANA) were 91.2, 79.4, 79.4, 70.6 and 23.5% respectively in group A. The positive rate of TRAb and TBII were significantly different between group C and group A or B (both P