RÉSUMÉ
Crotalaria ramosissima Roxb. (Fabales: Fabaceae) is a common weed that grows prolifically in few areas of Karnataka. The plant used as insect repellent in grain storage room and C. ramosissima leaves used to treat skin diseases. The purpose of study was to investigate phytochemical constituents and evaluate their antibacterial and antioxidant properties along with bioactive compound profiling. Phytochemical screening of ethyl acetate, ethanol and methanol extracts revealed presence of necessary phytochemical components, anti-microbial activity against plant pathogens showed best results from ethyl acetate extract with MIC 15.60µg mL-1 against Pseudomonas syringae and Xanthomonas oryzae with MIC 31.25µg mL-1, confirmed with TLC bio-autography, DPPH antioxidant assay, showed the highest activity of IC-50 2.71µg mL-1 from methanol extract with standard reference, Gas chromatography/Mass spectroscopy (GC-MS) used for profiling to detect chemical compounds from plant solvent extracts which showed presence of 21 compounds, ethyl acetate extract identified with 1,2,4-Oxidiazole, 3-(1,3-bezodioxol-5-y-5-[2-(4-methoxyphenyl)-ethyl] which is heterocyclic aromatic compound of azole family-alkaloid, which is reported for the first time in C. ramosissima. The results revealed significant properties and the obtained 1,2,4-oxidiazole derivative can be a novel bio-control agent against microorganisms and for crop protection. It also retained current researcher's attention from its biological properties in pharmaceutical drug industry.
RÉSUMÉ
Objective To stimulate the embryonic cells of the musca domestica with lipopolysaccharide(LPS) for production of antibacterial protein and to extract antibacterial protein ,then research the inhibitory action of the antibacterial protein and homohar‐ringtonine on human myeloid leukemia cells K562 and normal human cells .Methods The logarithmic growth phase′s embryonic cells of the musca domestica were stimulated using no serum M3 insect medium which contained 20 mg/L LPS for sixteen hours . The antibacterial protein was extracted from supernatant fluid .The antibacterial protein was prepared in 40 ,80 ,160 ,320 and 640μg/mL five density groups ;the MTT experiments were used to test the inhibition of antibacterial protein on K562 cells and the hu‐man umbilical vein vascular endothelial cells .The K562 cells and human umbilical vein vascular endothelial cells were prepared HTT and antibacterial protein of the embryonic cells of the musca domestica groups ,the normal control group was established by cells itself .Effective killing rate of K562 cells and the human umbilical vein vascular endothelial cells were measured .Results The effective inhibition ratio of homoharringtonine and the antibacterial protein on K562 cells and human umbilical vein vascular endo‐thelial cells on three density groups were detected by flow cytometry .The MTT examination demonstrated that all density antibac‐terial peptides had inhibition activities on K562 cells ,but no inhibition activities on human umbilical vein vascular endothelial cell . The effective killing and wound ratio of the control group ,the homoharringtonine group and of the antibacterial protein group from the embryonic cells of the musca domestica on the K562 cells were(28 .16 ± 2 .14)% ,(81 .41 ± 1 .95)% and (82 .90 ± 3 .03)% ,re‐spectively ;the effective killing rate on human umbilical vein vascular endothelial cells were(41 .13 ± 2 .51)% ,(82 .20 ± 2 .57)% and (36 .68 ± 1 .86)% ,respectively .Conclusion Compared with the common chemotherapeutics medicine ,the merit of the antibacterial protein from the embryonic cells of the musca domestica is that it can kill the tumor cells effectively ,but would not damage the nor‐mal person cells .