Gene coexpression analysis reveals dose-dependent and type-specific networks responding to ionizing radiation in the aquatic model plant Lemna minor using public data

Ionizing radiations (IRs) are widespread damaging stresses to plant growth and development. However, the regulatory networks underlying the mechanisms of responses to IRs remains poorly understood. Here, a set of publicly available transcriptomic data (conducted by Van Hoeck et al. 2015a), in which Lemna minor plants were exposed to a series of doses of gamma, beta and uranium treatments was used to perform gene coexpression network analysis. Overall, the genes involved in DNA synthesis and chromatin structure, light signalling, photosynthesis, and carbohydrate metabolism were commonly responsive to gamma, beta and uranium treatments. Genes related to anthocyanin accumulation and trichome differentiation were specifically downregulated, andgenes related to nitrogen and phosphate nutrition, cell vesicle transport, mitochondrial electron transport and ATP synthesis were specifically upregulated in response to uranium treatment. While genes involved in DNA damage and repair, RNA processing and RNA binding were specifically downregulated and genes involved in calcium signalling, redox and degradation of carbohydrate metabolism were specifically upregulated responding to gamma radiation. These findings revealed both dose-dependent and typespecific networks responding to different IRs in L. minor, and can be served as a useful resource to better understand the mechanisms of responses to different IRs in other plants.

Radiolytic studies of cefozopran hydrochloride in the solid state

Background: The radiation sterilization is one of the best methods for sterilizing vulnerable degradation drugs like cefozopran hydrochloride. Results: Chemical stability of radiosterylized cefozopran hydrochloride, was confirmed by spectrophotometric and chromatographic methods. EPR studies showed that radiation has created some radical defects whose concentration was no more than several dozen ppm. The antibacterial activity of cefozopran hydrochloride irradiated with a dose of 25 kGy was unaltered for Gram-positive bacteria but changed for two Gram-negative strains. The radiation sterilized cefozopran hydrochloride was not in vitro cytotoxic against human CCD39Lu normal lung fibroblast cell line. Conclusions: Cefozopran hydrochloride in solid state is not resistant to radiation sterilization and this method cannot be used for sterilization of this compound.

Beta therapy with 90strontium as single modality therapy for canine squamous cell carcinoma in third eyelid / Betaterapia com estrôncio-90 como modalidade única de terapia para o carcinoma de células escamosas na terceira pálpebra em cães

The purpose was to evaluate the effectiveness of beta-radiation with strontium-90 as single modality treatment of canine third eyelid squamous cell carcinoma (SCC). Nine dogs diagnosed with third eyelid SCC were treated with strontium-90. Radiation therapy was administered in four fractions of 100cGy per site every four days and at a depth of 0.2cm (Strontium-90 build' up) in each fraction. Radiation with beta therapy was well tolerated in all animals with no occurrence of radiation induced cataracts. In all cases, there were increased signs of conjunctival inflammation around the mass, which subsided with topical anti-inflammatory. Two dogs required surgical treatment for local tumor recurrence at 150 days and 352 days. In the remaining seven cases, disease free interval ranged from 1239 days to 2555 days. Beta therapy using 90Sr may be a valid alternative for the treatment of third eyelid SCC in dogs.

O objetivo foi avaliar a eficácia da betaterapia com estrôncio-90 como modalidade única de tratamento em carcinomas de células escamosas (CEC) da terceira pálpebra de cães. Nove cães foram diagnosticados com CEC de terceira pálpebra, que foram tratados com estrôncio-90. A radioterapia foi administrada em quatro frações de 100cGy por local, a cada quatro dias e a uma profundidade de 0,2cm em cada fração. A betaterapia foi bem tolerada por todos os animais, sem ocorrência de catarata induzida pela radiação. Em todos os casos, houve um aumento dos sinais de inflamação da conjuntiva ao redor da neoformação, as quais cederam com o uso de anti-inflamatório tópico. Em dois cães houve a necessidade de tratamento cirúrgico, pois houve recorrência local do tumor aos 150 dias e aos 352 dias. Nos outros sete casos, o intervalo livre de doença variou entre 1.239 dias e 2.555 dias. Betaterapia usando 90Sr pode ser uma alternativa válida para o tratamento do CEC da terceira pálpebra em cães.

Experimental model of the morphological and morphometric aspects of tissue repair in skin wounds submitted to beta radiation emitted by Strontium -90 in rats

The purperose of this work is tell the use of the experimental model to investigation of the effects of Beta radiation of estroncium-90 for repairing the tissue of wounds made in rats. 48 animals of the lineage EPM-1 Wistar were used, distributed in two groups that received radiation in alternate days (group A) and daily (group B). Each group was divided in four groups of six rats to be analysed in the 3rd, 7th, 14th and 21st day after operation. Two incisions were made in the back of the animal and sutured immediately afterwards; the upper part was irradiated and the lower part used as control. On the dates established the irradiated and control wounds were macroscopically examined and withdrawn for preparation of the histological comparative study in the optical microscope. Following, an morphometrical analysis was performed to count leucocyts, fiberblast and colagen fibers which were submitted to statistical study.

O objetivo deste trabalho é relatar o uso do modelo experimental para investigação dos efeitos da radiação Beta do Estrôncio-90 no processo de reparação tecidual de feridas provocadas em ratos. Utilizamos 48 animais da linhagem EPM-1 Wistar, distribuídos em dois grupos que receberam a radiação em dias alternados (Grupo A) e diariamente (Grupo B). Cada grupo foi subdividido em quatro, cada um com 6 ratos, para estudo no 3º, 7º, 14º e 21º dias de pós-operatório. Realizou-se duas incisões no dorso de cada animal, suturou-se em seguida, sendo que a de situação cranial foi irradiada e a caudal serviu para controle. Nas datas determinadas, as feridas controle e irradiada foram observadas macroscopicamente e retiradas para preparo do estudo histológico comparativo ao microscópico óptico. Realizou-se em seguida, análise morfométrica para contagem de leucócitos, fibroblastos e fibras colágenas, submetidos a estudo estatístico.