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{L-End}Objective To understand the influence of job burnout and anxiety symptoms on work ability of biosafety laboratory (BSL) workers in Xinjiang Uygur Autonomous Region. {L-End}Methods A total of 5 808 BSL workers in Xinjiang Region were selected as the research subjects using cluster sampling method. The Job Burnout Questionnaire, Anxiety Self-rating Scale and Work Ability Index Questionnaire were used to evaluate their job burnout, anxiety symptoms and work ability. The mediation effect of anxiety symptoms on the relationship between job burnout and work ability was tested by stepwise regression analysis. {L-End}Results The detection rate of job burnout, anxiety symptoms and poor working ability was 83.1% (4 829/5 808), 37.2% (2 160/5 808) and 5.8% (339/5 808), respectively. There was a positive correlation between job burnout and anxiety symptoms score in BSL workers ［correlation coefficient (r) was 0.48, P<0.01］. The score of job burnout and anxiety symptoms was negatively correlated with the work ability score (r were -0.58, -0.51, both P<0.01). The results of mediation effect analysis show that the total effect of job burnout on work ability was -0.31, with the direct effect of -0.15. The mediation effect of anxiety symptoms on the relationship between job burnout and work ability was -0.16, accounting for 51.6% of the total effect. {L-End}Conclusion The detection rates of job burnout and anxiety symptoms among BSL workers in Xinjiang Region are relatively high. Anxiety symptoms show a mediate effect on the relationship between job burnout and work ability. The work ability of BSL workers can be improved by improving job burnout and anxiety symptoms.
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Objective To understand the status of new coronavirus contamination in the biosafety laboratory environment, identify key areas prone to contamination, and provide evidence for disinfection of central objects. Methods surfaces of high-frequency contact environment and protective equipment were sampled with moistened sterile cotton swabs after experiment and before disinfection, the results of the one-step real-time reverse transcription polymerase chain reaction (RT-PCR) of open reading frame 1ab and N fragment were used to evaluate the pollution status. Results Environmental surveys found 4 of 217 samples of environmental objects to be positive for new coronavirus RNA, that positive rate was 1.84%. Among them, BSL-3, BSL-2, and BSL-1 were sampled 23, 184, and 10 respectively. The 3 positive samples were from surfaces of nucleic acid extraction room of BSL-2 and from the handles of pass-through box, laboratory door handles and the outer surface of the alcohol watering pot respectively. The 1 positive sample was from the forearm of the protective clothing in BSL-2 laboratory. Conclusion There was a certain degree of virus pollution in key areas of the new coronavirus laboratory. The BSL-2 laboratory has a higher risk of environmental pollution than the BSL-3 and BSL-1 laboratories.
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Objective Aims to reduce the hidden risks of laboratory biosafety,understand the status of biosafety laboratory record filing situations in Shenzhen,and also to provide scientific basis for further standardizing the management of biosafety laboratory in Shenzhen.Methods In 2017,75 laboratories in Shenzhen completed record filing were surveyed,method ologies adopted including application materials review,phone call consultation and communication,carrying out corrective ac tions based on feedback peer review suggestions and finally complete the record filing.Results The first/second level laborato ry of biosafety in shenzhen is mainly public medical institutions,followed by private hospitals.In 2017,the first three recordfiling LABS were Futian district,nanshan district and longgang district.According to the data analysis,lack of the second category of pathogenic microorganism laboratory activity project risk assessment report,and laboratory layout diagram function partition is not clear were two of the more prominent problems in the software and hardware of laboratory management respectively.Conclusions Basically,the overall record filing of Shenzhen biosafety laboratory is good,however,more measurements should be developed to deal with identified problems to further strengthen the standardized management of laboratory biosafety.
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This article describes the developing process of China’s pathogenic microorganism laboratory construction through a fair summary of the key points and events involving the issues of "Pathogenic Microbiology Laboratory Biosafety Management Regulations" , "National High Level Biosafety Laboratory System Construction Plan "and "High Level Biosafety Laboratory System Construction Plan" (2016-2025) " , the research and development of Domestic Mobile Biological Safety Level-3 Lab, the establishment and operation of Wuhan National Biosafety Laboratory and the release of other key technology and equipment development.
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Objective To develop an instrument for automatic testing of airtightness of the equipment and facilities in biosafety laboratory using pressure decay method with an aim at improving testing efficiency.Methods On the basis of the characteristics of the pressure decay method and the equipment performance in the biosafety laboratory,the PLC and touch screen and etc,industrial control instrument was employed for designing the structure and components.By programming PLC and touch screen the intended functions were achieved.Results This developed instrument could be used for automatic and multi-channel air tightness testing in parallel,as well as for monitoring and recording the pressure and temperature data of the equipment in real time.Conclusion By using the developed instrument,the testing cycle,and the manpower and material resources input can greatly be reduced.It is presumed that the instrument can be used for testing the airtightness of facilities and equipment in biosafety laboratory.
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Objective To develop an instrument for automatic testing of airtightness of the equipment and facilities in biosafety laboratory using pressure decay method with an aim at improving testing efficiency.Methods On the basis of the characteristics of the pressure decay method and the equipment performance in the biosafety laboratory,the PLC and touch screen and etc,industrial control instrument was employed for designing the structure and components.By programming PLC and touch screen the intended functions were achieved.Results This developed instrument could be used for automatic and multi-channel air tightness testing in parallel,as well as for monitoring and recording the pressure and temperature data of the equipment in real time.Conclusion By using the developed instrument,the testing cycle,and the manpower and material resources input can greatly be reduced.It is presumed that the instrument can be used for testing the airtightness of facilities and equipment in biosafety laboratory.
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In dynamic state,the airflow and particle distribution of class Ⅲ biosafety laboratory is simulated by means of the CFD.The identical results of the simulation and the practical survey show good reliability of this experiment.The result indicates that the good unidirectional air distribution is formed in main lab and air distribution is the main factors to determine the character of particle distribution.
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This paper mainly introduces some related problems of biosafety and biosafety laboratory. It also mentions the present situation of worldwide biosafety laboratory and gives out some proposals.
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The construction of biosafety laboratory was highly concerned these years because of the spread of high pathogenic diseases including SARS and avian influenza. In the past several years, the construction of many BSL-2/3 laboratories played an important role in preventing the dissemination of pathogens involved in the research. Meanwhile, serious problems were also raised because some project designers or constructors were not clear with the compulsory safety equipments in these laboratories so that the requirement of zero-leakage could not be achieved. The compulsory safety equipments emerging and disposing solid, liquid and gas offal in the biosafety laboratory were reviewed. The R&D of special equipments in biosafety laboratory in China was also introduced.
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Based on the engineering practice, the sealed design of key nodes for high-level biosafety laboratories(BSL-3 and BSL-4 Laboratory) are introduced including the selection and assemblage of panel materials of building enclosure, a variety of channels through walls, and inflatable airtight doors and efficient exhaust filtration unit, which can solve the gastightness questions of building enclosure. The test results of model laboratory show that natural attenuation of the pres- sure is less than 250 Pa in 20 min when he indoor air pressure up to 500 Pa.