Résumé
Objective:To explore the effect of inhibiting c-raf-1 by antisense technology on radiosensitivity in human cervical carcinoma cell line HeLa.Methods:There were four groups in the study:control group,lipofectin group,sense oligodexynucleotides(S-ODN) group,antisense oligodexynucleotides(AS-ODN) group.The expression of c-raf-1 was detected by means of RT-PCR,Cellular response to irradiation was evaluated by the colony forming test,Apoptosis was observed by fluorescent staining.Bcl-2 protein expression was determined by flow cytometry.Results:AS-ODN group could significantly decreased the proliferation rate and increasing the apoptosis rate,significantly downregulating the expression of Bcl-2 of irradiated human cervical carcinoma cells,(vs lipofectin group or S-ODN group,P
Résumé
Objective: To investigate the effects and the mechanisms of c-raf-1 genes antisense oligodeoxynucleotides(ASODN) transfection in inhibiting the human ovarian carcinoma SKOV3 cell lines.Methods: There were 3 groups in our study: normal control group,c-raf-1 sense oligodeoxynucleotides(SODN) experimental group,and c-raf-1 antisense experimental group.at the different time points after liposome-mediated transfection,the cell proliferation,apoptosis,protein expressing level were observed by MTT assay,flow cytometry,fluorescent microscope and cloning test.Results: In the ASODN experimental group and SODN group,the OD-value were 0.272 and 1.307 respectively(P
Résumé
Objective:To explore the effect of c-raf-1 antisense oligodeoxynucleotides (ASODN) treatment in the human ovarian epithelial cancer transplanted subcutaneously in nude mice.Methods:The models of human ovarian epithelial cancer transplanted subcutaneously were established in 15 nude mice,then divided randomly into 3 groups and different treatment were given respectively (control group,senseexperimental group and antisense experimental group).The weight of nude mice and tumor volume were observed,the tumor growth inhibitory rate and the tumor response rate calculated,too.Results:The growth inhibitory rate in sense experimental group and antisense experimental group were 6.8% and 68.1%,respectively,the tumor response rate of antisense experimental group was 16.7%.There was no statistical difference in nude mice weight among the 3 groups.Conclusion:The results suggest that there is a positive value in the human ovarian epithelial cancer transplanted subcutaneously in nude mice treated by c-raf-1 antisense oligodeoxynucleotides,which will be an important gene therapeutic strategy for the ovarian epithelial carcinoma in the future.