Résumé
Objective To investigate the application of theoretical K value and the calibration K value on Hitachi 7600 Automatic Biochemical Analyzer .Methods Rate method was employed to detect alanine aminotransferase (ALT ) ,aspartate aminotransferase (AST) ,gamma-glutamyl transpeptidase(γ-GT) ,creatine kinase(CK) and lactate dehydrogenase(LDH) .The theoretical K value and K values calibrated by two kinds of calibration serum were recorded as K ,K1 ,K2 and the three kinds of calibration results of enzymes mentioned above were compared .Results The theoretical K values of ALT ,AST ,γ-GT ,CK and LDH were all less than their two kinds of actual K values .The results of the calibration K value were significantly superior to that of the theoretical K value .The calibration results R2 value of five kinds of enzymes were all greater than 0 .95 .Conclusion The theoretical K value is not suitable for clinical laboratories ,and the two kinds of calibration serum can meet the needs of quality control and clinical work .