Résumé
To clarify the functions of Coix-seed Reactive Derivatives(CRD), we analyzed 13 kinds of phenolic compounds and coixol(an alkaloid) in CRD powder by using Liquid Chromatography/Mass Spectrometry(LC-MS).The following are the results: p -Coumaric acid 275.0 (μg/g), p -Hydroxybenzaldehyde 50.2 (μg/g), Caffeic acid 36.9 (μg/g), Protocatechuic acid 31.9 (μg/g), Ferulic acid 27.1 (μg/g), Quercetin 16.2 (μg/g), Syringaldehyde 12.1 (μg/g), Chlorogenic acid 11.3 (μg/g), Naringenin 11.1 (μg/g), Vanillin 9.2 (μg/g), trans -Coniferylaldehyde 9.1 (μg/g), Tricin 0.7 (μg/g), Luteolin 0.5 (μg/g) and Coixol(6-Methoxy-2-benzoxazolinone) 5.5 (μg/g). CRD is composed of many substances other than these phenoic compounds. Therefore, further studies are needed to understand the various clinical features associated with CRD intake.
Résumé
Objective: To compare the differences of contents of effective components triolein and coixol in Coix lacryma-jobi var. mayuen from different origins. Methods: The Discover C18 column was used for the detection of triolein with mobile phase of acetonitrile-dichloromethanol (66:34). The flow rate of mobile phase was 0.7 mL/min. The column temperature was controlled at 30℃. The ELSD was used. The tube temperature was 45℃, and the flow rate of nitrogen was 1.5 L/min. The Agilent Zorbax SB-C18 column was used for the detection of the coixol with the mobile phase of acetonitrile-water (25:75), a flow rate of 1 mL/min, a temperature of 25℃ and a wavelength of 232 nm. Results: The triolein content of Zhejiang province was the highest, at 1.04%, and the lowest was in Hainan Province, which was 0.53%; The highest coixol content was in Shanghai, reaching 3.469 7 mg/g, but the lowest content was only 0.619 3 mg/g in Anhui province. Conclusion: The contents of triolein and coixol in adlay from eight Provinces are significantly different, the contents of triolein and coixol in adlay from Zhejiang and Fujian Provinces were relatively high, and medicinal material quality is better.
Résumé
Hot water extract of adlay (<i>Coix lachryma-jobi</i> L. <i>var. ma-yuen</i> Stapf) seed, commonly called Yokuinin, has been used as herbal medicine for treating verruca vulgaris, et al. Although there have been a number of studies on the usefulness of Yokuinin, the pharmacological assessment of its husk, pellicle, and astringent skin remains unclear. In this line, we evaluated the effect of methanol extract from all parts of adlay grain (seed, husk, pellicle, astringent skin) on cancer cells and identified its useful chemical components. Results revealed that a fraction of the extract have weak growth-suppressing activity on human cervical cancer cell line (HeLa cell). In particular, 5,7-dihydroxychromone and coixol were isolated and identified from the active fraction. This indicates the possible cancer chemopreventive efficacy of methanol extract from adlay. Moreover, further tests are needed to determine the role of 5,7-dihydroxychromone.<br>