Résumé
Objective: To screen out the molecular mechanism of core herbal pair “Astragali Radix-Angelica Sinensis” for treating consumptive disease by using the Traditional Chinese Medicine Inheritance Support System (TCMISS) and network pharmacology. Methods: The prescriptions and commonly used compatibility combinations containing Astragali Radix, and their main treatment of diseases were excavated from TCMISS database, and the core compatibility of “Astragali Radix-Angelica Sinensis” against consumptive disease was illustrated. The herb-ingredients-targets network was constructed through network pharmacology referring to Batman-TCM, GeneCards, OMIM, and so on. Meanwhile, the topology, cluster analysis, GO, and KEGG pathways analysis of the targets were performed. Results: TCMISS database contained totally 459 prescriptions containing Astragali Radix involving 641 herbs. “Astragali Radix-Angelica Sinensis” was the core compatibility for the treatment of consumptive disease, which was consistent with ancient record and clinical medication. The results also showed that the process of immune response, oxidative stress, and signal transduction were its mainly molecular mechanism, by adjusting the pathways in cancer, PI3K-Akt signaling pathway to play its treating consumptive disease effect. Conclusion: The paper revealed the molecular mechanism of “Astragali Radix-Angelica Sinensis”, and provided theoretical basis and reference for the follow-up study of Astragali Radix and its prescriptions.
Résumé
By using the traditional Chinese medicine inheritance support system (TCMISS) in this study, the prescription rules of Baizhi formulae were analyzed and the core herbal pair "Baizhi-Chuanxiong" was obtained. Through the systemic analysis of prescription rules of "Baizhi-Chuanxiong" and combined with the pharmacology thinking of "Baizhi-Chuanxiong" in treating headache, the paper was aimed to find out the combination rules containing Baizhi andits molecular mechanisms for treating headaches, and provide the theory basis for further research and reference of Baizhi and its formula. Totally 3 887 prescriptions were included in this study, involving 2 534 Chinese herbs. With a support degree of 20% in analysis, 16 most commonly used drug combinations were screened, which were mainly used to treat 15 types of diseases. Baizhi was often used to treat headache, and the core combination "Baizhi-Chuanxiong" was also often used to treat, consistent with ancient record. A chemical database was established; then the headache and migraine disease targets were retrieved and added in the database to build up the "compounds-targets-pathways "core network of "Baizhi-Chuanxiong" by the internet-based computation platform for IP of TCM (TCM-IP). TCM-IP was then applied to study the molecular mechanism of "Baizhi-Chuanxiong" treatment of headache. The results suggested that37 chemical compounds in the core combination "Baizhi-Chuanxiong" were closely related with headache treatment by adjusting serotonin levels or applying to inflammation-related targets and energy metabolism pathways such as purine metabolism, pyruvate metabolism, fatty acid degradation, carbon metabolism and gluconeogenesis.
Résumé
This study was aimed to analyze the current prescription combination rules on traditional Chinese medicine (TCM) in gastric cancer treatment, in order to explore reasonable application of data mining technology in the study of prescription combination rules. Modern literatures were searched in CNKI and WanFang database. Frequency analysis, association analysis and composition network were used comprehensively. Rules such as herb application frequency and combination rules of the target prescription set were analyzed. The results showed that after screening and standardization, 116 prescriptions were included in the set which including 1 269 herbs and involving 17 types of 212 herbs. It was concluded that TCM paid attention to strengthen body resistance in gastric cancer treatment. It can also be combined with drugs for invigorating blood circulation and stasis, heat-clearing and detoxification, dispersing phlegm and stasis, as well as regulatingqi stagnation depending on the syndrome.