Résumé
OBJECTIVE: In order to investigate the role of VEGF and bFGF in endometrial stromal sarcoma(ESS). METHODS: We studied the effect of VEGF and bFGF on proliferation of endometrial stromal sarcoma cell after adding VEGF and bFGF into a culture system by autoradiography. The incorporation of [H3]-thymidine into cellular DNA was used as an index of cellular proliferation. RESULTS: VEGF and bFGF significantly stimulated the proliferation of endometrial stromal sarcoma cells. The comparison of the effects of VEGF and bFGF showed that there was slightly more potent growth effects on endometrial stromal sarcoma cells with bFGF however, there was no stastistical significance. CONCLUSION: Taken together, these results suggest that VEGF and bFGF play an essential role in endometrial stromal sarcoma growth and our data adds new insights in the pathogenesis of ESS. This suggests that the involvement of growth factors may not only angiogenesis, but also play a critical role in controlling ESS cell growth.
Sujets)
Autoradiographie , Prolifération cellulaire , ADN , Facteur de croissance fibroblastique de type 2 , Protéines et peptides de signalisation intercellulaire , Sarcome du stroma endométrial , Facteur de croissance endothéliale vasculaire de type ARésumé
OBJECTIVE: In order to investigate the role of VEGF and bFGF in endometrial stromal sarcoma(ESS). METHODS: We studied the effect of VEGF and bFGF on proliferation of endometrial stromal sarcoma cell after adding VEGF and bFGF into a culture system by autoradiography. The incorporation of [H3]-thymidine into cellular DNA was used as an index of cellular proliferation. RESULTS: VEGF and bFGF significantly stimulated the proliferation of endometrial stromal sarcoma cells. The comparison of the effects of VEGF and bFGF showed that there was slightly more potent growth effects on endometrial stromal sarcoma cells with bFGF however, there was no stastistical significance. CONCLUSION: Taken together, these results suggest that VEGF and bFGF play an essential role in endometrial stromal sarcoma growth and our data adds new insights in the pathogenesis of ESS. This suggests that the involvement of growth factors may not only angiogenesis, but also play a critical role in controlling ESS cell growth.