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Introduction: Carcinoma of the stomach is a disease with agrave prognosis. The revelation of the genetic and molecularbasis of gastric cancer has helped the development of targetedtherapies which has the potential to improve survival.Decreased expression of Epithelial cadherin (E-cadherin)in gastric carcinoma is one such genetic alteration whichcould help in targeted therapy and prognostication. Theobjective of the present study was to identify E- cadherinimmunohistochemical expression in gastric carcinoma and itscorrelation with histopathological features.Material and Methods: Gastric biopsies and surgicalspecimens from a tertiary care center in South Indiawere included and assessed by light microscopy andimmunohistochemistry (IHC).Results: Aberrant E-cadherin staining was seen in 42.8% ofgastric adenocarcinoma. Aberrant staining was found withdisproportionately high frequency in diffuse-type (95.5%)when compared to intestinal-type (18.6%) and mixed type(20%) with very high statistical significance with P <0.001. All(100%) poorly differentiated tumors had aberrant E- cadherinstaining while only 21.2% of moderately differentiated and12.5% of well-differentiated tumors had aberrant E-cadherinstaining and this was statistically highly significant with P<0.001. In this study, there was no significant associationbetween E-cadherin with age, gender, nature of the specimen,site of the tumor, size of the tumor, lymph node status andtumor invasion.Conclusion: This study emphasizes the importance ofidentification of aberrant E-cadherin expression in gastriccarcinomas which in turn helps in selecting patients for noveltherapies which might improve the prognosis of this gravedisease.
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Objective: To investigate the targeting relationship between microRNA-153-3p and transforming growth factor-beta 2 (TGFβ2) and its effects on invasion, migration and epithelial-mesenchymal transformation of cultured glioma cells. Methods: Real-time fluorescence quantitative PCR and Western blotting were used to detect the effects of miR-153-3p on the expressions of TGFβ2 mRNA and protein. Bioinformatics software was used to predict the binding sites of miR-153-3p to TGFβ2. Luciferase reporter assay was used to analyze the targeting relationship between miR-153-3p and TGFβ2. Transwell and wound healing assays were used to detect the effects of miR-153-3p on SHG-44 cells invasion and migration. Western blotting was used to detect the expressions of proteins related to invasion, migration and epithelial-mesenchymal transition. Results: miR-153-3p mimic inhibited the expressions of TGFβ2 mRNA and protein in SHG-44 cells. Software predicted results showed a continuous binding region between miR-153-3p and TGFβ2. miR-153-3p mimic co-transfected with TGFβ2 wild-type reporter vector significantly decreased the activity of luciferase in the cells (P<0.01). miR-153-3p mimic co-transfected with TGFβ2 mutant reporter showed no significant change in luciferase activity. The number of invasive cells and scratch closure rate of SHG-44 cells transfected with miR-153-3p mimic were significantly decreased. The expression levels of MMP-2, MMP-9 and VEGF were significantly decreased. The expression levels of N-cadherin, Snail-2, Vimentin and Twist were significantly decreased, and the expression level of E-cadherin was significantly increased (P<0.01). The high expression of TGFβ2 alleviated the effect of miR-153-3p on the cell invasion and epithelial mesenchymal transition related proteins in SHG-44. Conclusion: miR-153-3p inhibits the invasion and migration of cultured glioma cells and epithelial mesenchymal transition by targeting TGFβ2.
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Context: Gastric adenocarcinoma (GAC) is a common malignancy with high mortality-rate. Analysis of molecular markers could form a foundation for the future use of targeted therapies to reduce morbidity and mortality. Aims: To fi nd the prevalence and relation of epithelial cadherin (E-cadherin) and human epidermal growth factor receptor 2 (HER-2/neu) protein expression with histological type and grade of GAC using immunohistochemistry (IHC). Materials and Methods: A total of 100 cases of GAC diagnosed over a 2 year period were studied. Expression of E-cadherin and HER-2/neu was analyzed by IHC in relation to the histological type and grade. Results: Of the 100 cases of GAC studied, 11 revealed a loss of E-cadherin and over-expression of HER-2/neu was seen in 17 cases. Loss of E-cadherin was seen in 50% of signet ring-cell carcinomas but only in 8% of tubular and none of papillary and mucin-secreting GAC (P = 0.003). Of all the cases of tubular GAC with loss of E-cadherin expression, majority (71.4%) were Grade III (P = 0.04). Of all the tubular GAC cases with an over-expression of HER-2/neu, 20% and 67% were Grade I and II GAC respectively while only 13% were Grade III (P < 0.001). Conclusions: Although poorly-differentiated tumors show loss of E-cadherin, better-differentiated tumors over-express HER-2/neu protein. Signet ring-cell carcinoma is more likely to exhibit a loss of E-cadherin protein. Targeted therapy toward HER-2/neu in GAC should be considered. Novel therapy to block E-cadherin down-regulation is justified.
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Objective To explore the expressions and clinical significance of epidermal growth factor receptor (EGFR),COOH-terminus tensin-like molecule (CTEN)and epithelial cadherin (E-cad)in non-small cell lung cancer (NSCLC).Methods The expressions of EGFR,CTEN and E-cad in 36 cases of normal lung tissue and 82 cases of NSCLC tissues were observed with immunohistochemical SP method and their correlation with NSCLC invasion,metastasis and prognosis was analyzed.Results The positive rate of EGFR,CTEN and E-cad was 58.5% (48/82),69.5% (57/82)and 28.1% (23/82)in 82 cases of lung cancer,while 13.9% (5/36),0.0% (0/36)and 100% (36/36)in normal tissues;the differences were all significant (P<0.05).EGFR,CTEN and E-cad expressions in NSCLC tissues were significantly correlated with tumor differentiation,TNM stage and lymph node metastasis (P<0 .0 5 ).The expressions of EGFR and CTEN were correlated with each other in NSCLC (r=0 .5 3 0 , P<0.001),while the expressions of EGFR and CTEN were correlated with that of E-cad (r=0.499,P<0.001;r=0.333,P=0.001 ).Multivariate Cox regression analysis showed that CTEN expression in NSCLC was an independent prognostic factor (P<0.05 ).Conclusion EGFR,CTEN and E-cad may play a role in the development,invasion and metastasis of NSCLC and have some significance in predicting the prognosis of NSCLC.
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Objective To investigate the correlation between serum soluble epithelial cadherin (sE-cad) and postoperative recurrence and prognosis in patients with advanced gastric cancer.Methods The level of serum sE-cad in 85 patients with advanced gastric cancer (advanced gastric cancer group) was detected by ELISA technique preoperative and postoperative 1 month,and compared with 30 healthy controls(control group).The patients in advanced gastric cancer group were followed up for 3 years,the level of serum sE-cad in recurrent patients and non-recurrent patients was compared.Results The level of serum sE-cad in advanced gastric cancer group preoperative was significantly higher than that in control group [(24.3 ± 14.8) μ g/L vs.(9.4 ± 3.8) μ g/L,P ＜ 0.01].The level of serum sE-cad in advanced gastric cancer group was significantly decreased postoperative 1 month [(12.5 ± 6.4) μ g/L vs.(24.3 ± 14.8) μ g/L,P ＜0.01].The level of serum sE-cad in recurrent patients was significantly higher than that in non-recurrent patients and postoperative 1 month [(20.7 ±9.8)μg/L vs.(12.5 ±6.4),(14.8 ±6.2) μg/L,P＜0.01].Univariate analysis revealed that preoperative high serum sE-cad level was related with tumor size,differentiated degree,lymph node metastasis ratio,depth of tumor invasion (P ＜0.05),but had no relationship with histological type(P＞ 0.05).Elevated preoperative serum sE-cad level negatively affected the postoperative survival rate and recurrence rate.Multivariate Logistic regression anaiysis revealed that preoperative serum sE-cad level was an independent risk factor for postoperative 3 years survival rate in advanced gastric cancer (HR =2.068,P =0.013).Conclusions Preoperative elevated serum sE-cad level is related with pathologic features in patients with advanced gastric cancer,and may be an important prognostic factor.Postoperative monitoring the level of serum sE-cad is useful for evaluating the prognosis and recurrence.
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ObjectiveTo examine the expressions of amplified in breast cancer 1(AIB1) and epithelial cadherin (E-cadherin) in ovarian carcinoma (OC) tissues,and determine the correlation between the expression and clinical pathological features.MethodsThe expression of AIB 1,E-cadherin,estrogen receptor (ER),progesterone receptor (PR) and Ki-67 in tissues of 50OCs and 13 normal ovarians tissues were detected by immunohistochemistry(IHC) EnVision two step process analysis.ResultsPositive expression of AIB1 in OC tissues[68％(34/50) ] was obviously higher than that in normal ovarian tissues [8％ (1/13)] (P ＜0.01).Down-regulation of E-cadherin expression was 60％ (30/50).The positive expression of AIB1 was significantly higher in stage Ⅲ and Ⅳ than in stage Ⅰand Ⅱ according to International Federation of Gynecology and Obstetrics (FIGO) stage (P =0.036),in lymph node metastasis group than in none lymph node metastasis group ( P =0.027 ),in stage G3 than in stage G1 and G2 according to Silverberg stage (P =0.003),and in serous adenocarcinoma group than in non-serous adenocarcinoma group (P=0.049);positive rates of ER and Ki-67 were higher than negative rates of ER(P=0.000) and Ki-67 (P =0.009) respectively.Down-regulation of E-cadherin expression was higher in FIGO stage Ⅲ and Ⅳ than in stage Ⅰ and Ⅱ (P =0.044),in serous adenocarcinoma group than in non- serous adenocarcinoma group ( P =0.022) ; positive rates of ER and Ki-67 were higher than negative rates of ER ( P =0.02 1 ) and Ki-67 (P=0.035) respectively.The expression of AIB1 was negatively correlated with E-cadherin expressioh (P =0.026).ConclusionsThe expressions of AIB1 and E-cadherin in OC tissues is closely related to clinical stage.Therefore,AIB1 and E-cadherin may be important moleculars involved in the progression of OC.
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Objective To investigate the expressions of RGC-32 and E-cadherin in pancreatic cancer and analyze their clinicopathological significance and the correlation with each other.Methods SP immunohistochemistry was used to detect the expressions of RGC-32 and E-cadherin in 42 cases of pancreatic cancer tissues,12 cases of chronic pancreatitis tissues and 8 cases of normal pancreatic tissues.Results The positive staining for RGC-32 was predominantly observed in the cytoplasm of pancreatic acinar cells.The positive staining for E-cadherin was mainly observed in the cytomembrane of normal pancreatic and chronic pancreatitis acinar cells,but aberrant expression ( cytoplasm expression and ( or ) weaker expression) could be found in pancreatic cancer cells.The positive expression rate of RGC-32 and aberrant expression rate of E-cadherin were 78.6％ (33/42) and 54.8％ (23/42),respectively,in pancreatic cancer tissues,which were significantly higher than those in normal pancreatic tissues [37.5％ (3/8) and 0] and chronic pancreatitis [41.7％ (5/12)and 8.3％ (1/12) with statisticai significance,P ＜0.05].The expression of RG C-32 in pancreatic cancer was associated with lymph node metastasis and TNM staging (P =0.016,0.025,respectively),but not with age,gender and differentiation degree ( P =0.831,1.000,0.629,respectively).The aberrant expression of E-cadherin was associated with differentiation degree,lymph node metastasis and TNM staging ( P =0.024,0.004,0.004,respectively),but not with age and gender ( P =0.970,1.000,respectively).A significantly positive correlation was found between positive expression rate of RGC-32 and aberrant expression rate of E-cadherin (r =0.458,P ＜0.01 ).Conclusions Both positive expression rate of RGC-32 and aberrant expression rate of E-cadherin are up-regulated significantly in pancreatic cancer tissues and RGC-32 may be involved in the invasion and metastasis of pancreatic cancer by regulating epithelial mesenchymal transition.
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Objective: To investigate CDH1 methylation of epithelial cadherin(E-cadherin) gene in intrahepatic cholangio carcinomas (ICCs). Methods: Forty-two liver samples were obrained from ICC patients (32 males and 10 females) during surgical resection in Eastern Hepatobiliary Surgery Hospital. The ICC tissue samples and the adjacent tissue samples were paraffin-embedded and fresh ice-frozen. A methylation-specific polymerase chain reaction (MSP) was used for analyzing the methylation of CDH1 gene; E-cadherin protein and mRNA expression was detected by immunohistochemical method and RT PCR analysis, respectively. Results: The methylation rate of CDHI was 28.6% in ICC patients. The expression of E-cadherin mRNA and protein was decreased in 64.3% and 69.1% of the samples, respectively. The methylation of CDH1 gene was correlated with the expression of E-cadherin protein and mRNA and metastasis of ICCs (P=0.008, P=0.031, and P=0.020, respectively), but not with the prognosis of ICC. The abnormal expression of E-cadherin was significantly correlated with the survival of patients (P=0.002). Conclusion: The methylation of CDH1 gene and down-regulation of E-cadherin are frequently seen in ICC patients, indicating that they may be closely related to the development and progression of ICCs.
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Objective To study the expression of cyclooxygenase-2,epithelial cadherin and vascular endothelial growth factor-c in colorectal cancer and its significance. Methods Seventy-seven patients with colorectal cancer were detected the expression of COX-2, E-ca nd VEGF-c by immunohistochemical SP method. Results COX-2 positive expression in colorectal cancer tissue was 24.7%; E-ca positive expression rate of 68.8%; VEGF-c expression rate of 84.4%. COX-2 in highly differentiated cancer tissues was significantly higher than in poorly differentiated ones (P ＜0. 05); E-ca in the tubular adenocarcinoma was higher than in the mucous glands cancer and other types of cancer ( P ＜ 0. 05 ); The Positive expression rate of E-ca in the stage Ⅰ + Ⅱ was significantly higher than stage Ⅲ + Ⅳ (P ＜0. 005). COX-2 protein expression and VEGF-c expression was negatively correlated (P ＜ 0. 05 ). Conclusion Combined detection of COX-2,VEGF-c and E-ca is helpful to recognize the occurrence, development and tratment of colorectal cancer.
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Objective To investigate the value of soluble epithelial cadherin(sE-cad)in the differential diagnosis of pleural effusion. Methods Patients were divided into malignant pleural effusion group,infective pleural effusion group and transudation group.sE-cad in pleural fluids obtained during the first thoracocentesis was measured by enzyme-linked immunosorbent assay(ELISA).The concentration of sE-cad in all kinds of pleural effusions was compared.The cut-off value of sE-cad for the differential diagnosis of benign and malignant pleural effusion was determined by ROC curve.The diagnostic value of sE-cad was also compared with common tumor markers such as CEA,CA199,CA125 and NSE.Results The concentration of sE-cad was significant higher in the malignant pleural effusion than in the benign pleural effusion[(38.38?4.15)ng/mL vs(14.17?0.80)ng/mL,P
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Objective To observe the effects of Chinese herbal medicine of Yiqi Huoxue (YQHX) on proliferation and expression of E-CD and invasion of human lung cancer cell lines - small cell lung cancer (NC-H446) in vitro. Methods By cell measured method, immunohistochemistry method, technique of image and Boyden Chamber method, the effects of YQHX or all trans-tretinoin acid (ARTA) on proliferation and expression of E-CD and invasion of NC-H446 cell lines with different time cultured by YQHX or ARTA in vitro were studied. Results The proliferation of NC-H446 were inhibited significantly by 5% YQHX or 10-5 mmol/L ARTA (P0.05). The shape and structure of cell NC-H446 was occurred variant. YQHX enhanced remarkably expression of E-CD of NC-H446 with 36 hour cultured (P 0.05). The quantity of NC-H446 cells crossed Matrigel and polyvinylpyrrolidone-free polycarbonate filter were more than the control group (P 0.05). Conclusion YQHX can inhibit the proliferation, enhance expression of E-CD on NC-H446, and decrease invasion and metastasis of NC-H446.
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OBJECTIVE: To study the correlation between serum soluble epithelial cadherin(sE-Cd) of patients with gastric carcinoma(GA) and the invasion and recurrence of gastric cancer.METHODS: Concentrations of soluble E-cadherin in 20 healthy adults,20 recurrent cases after GA surgery and 80 GA cases at 0,7 and 14 days after operation were determined by enzyme linked immunosorbent assay so as to explore the regular pattern;the correlation between sE-Cd level and the pathology and clinical features of GA was analyzed,and the carcinoembryonic antigen(CEA) and CA19-9 which served as controls were detected.RESULTS: Serum concentrations of sE-Cd were higher in the patients with postoperative relapse of gastric carcinoma than in normal control(P
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AIM: To explore the expression of E-cadherin(E-cd) in the human bronchial epithelial cells(HBECs) and their role in the damage and injury of the airway.METHODS: The E-cd protein expression and the Ecd mRNA of HBECs were determined by immunocytochemistry analysisand RT-PTR analysis,respectively.RESULTS: Ozone-stress caused a decrease in the HBECs membrane E-cd expression and an increase the cytoplasmic E-cd expression(P(0.05)).CONCLUSION: E-cd is distributed on HBECs.O_3-stress decreases the membrane E-cd expression but increases the cytoplamic E-cd expression.The change of E-cd expression may mediate HBECs injuries induced by O_3-stress.
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Objective To study the expression and significance of CD44v6 and E-Cadherin( E-cd) in human renal cell carcinoma. Methods Immunohistochemical method was used to detect CD44v6 and E-cd expression in 20 normal renal tissues and 72 primary renal cell carcinoma tissues. Results The postive expression rate of CD44v6 in normal renal tissues and primary renal cell carcinoma tissues was 15 00% and 66 67% respectively with significant difference (P
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Purpose:To investigate the expression of E cadherin and nm23 in papillary thyroid carcinoma (PTC) and its clinical significance. Methods:The expression of E cadherin and nm23 were detected by SP immunohistochemical technique in 70 paraffin embedded tissue specimens of PTC.Results:The positive rates of E cadherin and nm23 were 44.3%(31/70) and 62.9%(44/70) respectively. There were close relations between the expression of E cadherin and nm23 and pathological grade, TNM stage and lymph node metastasis, and no difference could be found between the expression of E cadherin and nm23 and size of tumor, sex and age of patients. There was distinct correlation between the expression of E cadherin and nm23. The metastatic rate in the group, which the expression of E cadherin and nm23 were both positive, was 12%(3/25), but in the all negative group, the metastatic rate was 75%(15/20). There was significant difference between the two groups ( P