Résumé
Objective: To study the effect of Yinhuangshen essential oil in mice of solar dermatitis model by ultraviolet (UVB). Method: Sixty SPF BALB/c female mice were randomly divided into blank group, model group, Minlixiao group, and large and small-dose Yinhuangshen essential oil groups. Except for blank group, model group was given 0.25 mL·cm-2 normal saline on the back every day. The Minlixiao group was given Min Lixiao every day. Low-dose Yinhuangshen essential oil group was given 0.125 mL·cm-2, and large-dose group was given 0.25 mL·cm-2, once a day, and the corresponding drugs were given continuously for 8 days. Except for blank group, all of the other groups were irradiated for 1.5 hours at 15 cm away from 308 nm UVB lamp on the 6th day. One hour and 24 hours after irradiation, mice were given corresponding drug once, and skin was observed 48 hours later. Contents of nitric oxide (NO), nitric oxide synthase (NOS) and tumor necrosis factor-alpha (TNF-α) in some local skin homogenates were measured. The histopathological changes were observed by htoxylin eosin(HE) staining. Result: Compared with blank group, skin of model group was reddish, swollen and ulcerated, epithelium and adnexal were absent, a large number of inflammatory cells and necrosis were adhered to the surface, the local histopathological changes significantly (Pα were significantly increased (Pα in the skin tissue of Minlixiao group and Yinhuangshen essential oil group were significantly decreased, and it could remarkable reduce the pathological changes in local tissue morphology (PConclusion: Yinhuangshen essential oil has a good effect in preventing and treating solar dermatitis.
Résumé
The external treatment of traditional Chinese medicine(TCM) is the earliest known treatment method for diseases. It originated in pre-Qin dynasty, grew up in Han and Tang dynasties, innovated in Song and Yuan dynasties, and developed in Ming and Qing dynasties. It has made outstanding contributions to the human health.With the rapid rise of modern drug-derived diseases, the external treatment of drugs has attracted wide attention at home and abroad due to its small adverse reactions, convenient application and accurate curative effect. With the increasing of TCM for external use drugs, dosage form, method, the clinical application of TCM has been expanding unceasingly, it has been widely applied to the pediatric, gynecological, internal medicine, oncology, however, there has not many specifications for amount, usage, time, and course of treatment, which are more arbitrary. Topical use of TCM can play a direct role in the treatment of local lesions. The external use of TCM is mainly explained by "the principle of external treatment is the principle of internal treatment", and the internal use function can only be explained from the theoretical part of TCM on its external treatment characteristics, and most can not well guide the corresponding clinical application of TCM. There are few reports on the mechanism of external use of TCM, and most of them are from theory to theory, lacking the confirmation and support of modern studies. There are also related reports on transdermal absorption of scattered TCM for external use. However, most of the external use of TCM are crude preparations such as pulverising, decocting in water and alcohol extraction, and its more random which the dose, dosage type, course of treatment, external use methods, coupled with its complex ingredients and low content, it is difficult to explain the mechanism of external use simply from transdermal absorption, nor does it meet the facts of clinical application of TCM. The mechanism of external use is not clear, and restricted the development of TCM for external use. This article through the extensive experimental research, literature research and database research, and proposed that TCM for external use through adjustment of neural-endocrine-immune network disorders, through local micro effect, micro stimulation, micro absorption, that's the "three fine tuning balances", and exert external use curative effect, so as to achieve the purpose of treating diseases.
Résumé
With a clear efficacy in external treatment of diseases, traditional Chinese medicine(TCM) has been widely applied in surgery, in acute, pediatric, gynecological, internal medicine and other departments. Its advantages include easy use, direct access to lesions, and prevent of the first-pass effect of the liver. However, its mechanism of action is still unclear, which impacts its clinical promotion. This article makes analysis and summary on the clinical application of TCM in external treatment of diseases, the characteristics of commonly used TCM in treatment of diseases, and the relationship of the common externally treated diseases and the "neurological-endocrine-immune network". The findings showed that TCM for external treatment were widely used, with exact curative effects; the pathogenesis of commonly used TCM for externally treating diseases is closely related to the "neuro-endocrine-immune network". Moreover, the modern pharmacological effects of TCM for externally treating diseases were found to be 100%related to the "neuro-endocrine-immune network". According to the analysis of the relationship between the common diseases externally treated with TCM and the "neuro-endocrine-immune network", the pathogenesis of the treatment with TCM is closely related to the "neuro-endocrine-immune network". Therefore, we believe that the "neuro-endocrine-immune network" system is probably one of the main mechanisms of action of TCMs for externally treating diseases. The verification of this mechanism requires the joint efforts of various departments in conducting clinical or experimental verification in the future, so as to provide a reference for clarifying the mechanism of TCM for external use, and basis for the promotion and application of TCM for external use.