Résumé
Objective To investigate the immunological reaction,serum and liver ?-L-iduronidase(IDUA) activity after human fetal liver cells(FLCs) transplantation therapy on mucopolysaccharidosis(MPS) which attribute to IDUA defect.Methods FLCs were transplanted into IDUA deficiency mice.T cell subsets(CD3,CD4 and CD8) was detected by flow cytometry,while IL-2,TNF-? and IFN-? by ELISA,and serum and liver IDUA activity by fluorospectrophotometer at different stage(before transplantation and 5,10,15 days after transplantation).Results Human FLCs survived in IDUA deficiency mice,bringing elevated serum and liver enzyme activity to receptor.T cell subsets,IL-2,TNF-? and IFN-? was significantly higher 5,10 and 15 days after transplantation than those before transplantation(P
Résumé
This paper describes the study on intramedullary injection of fetal liver cell suspension into the lethally irradiated mice. The results show that the therapeutic effect of intramedullary injection is better than that of intravenous one.It is suggested that the fetal liver cells injected into the bone marrow cavity can settle down and proliferate here and the volume of injection should be limited.
Résumé
Fifteen patients with hepatocellular failure from viral hepatitis were treated with general combined therapy and transfusion" of homologous fetal hepatic cells (FLO for 36 times (group A). An additional 15 cases with hepatocellular failure from viral hepatitis were treated only with general combined therapy, as control (group B). Twelve cases of the group A were diagnosed by transcutaneous liver biopsy.The results showed that FLC suspension achieved satisfactory effects and those who received FLC rallied remarkably better than those who did not. The fatality rates of group A and B were 26.7% (4/15) and 60.0% (9/15) respectively. In group A after transfusion of FLC, the effects were mainly shown by the elevation of the prothrombin activity (elevated for 16.5% after 24 h and for 18.2% after 48 h in average), recovery from hepatic coma and improvement in the subjective symptoms and various hepatic function tests. In survivals of group A (11 cases), the serum bilirubin returned to normal more quickly than that of group B (5 cases), being 55.4?24.5 days and 119.7?54.9 days, respectively (P