Résumé
Objective: To investigate the effect of CD44 fucosylation on fluid adhesion force of rabbit bone marrow mesenchymal stem cells (BMSCs).
Résumé
Objective To establish a platform for enrichment and purification of core fucose glycosylation and analyse its immu‐nity properties .Methods Serum immunoglobulin G(IgG) was seperated and purified by using protein G cross‐linked sepharose ,and core fucose glycosylation components of IgG were enriched and purified by using lens culinaris agglutinin (LCA)affinity chromatog‐raphy ,meanwhile ,the isoforms of LCA‐IgG were determined by using Western blotting .Results The purification rate of serum IgG was 62% and yield rate of LCA‐IgG was 4 .59% .Serum IgG1 ,IgG2 and IgG3 were identified except IgG4 in patients with schizo‐phrenia ,while serum IgG1 ,IgG2 ,IgG3 and IgG4 were all identified in healthy individuals .Conclusion A method for enrichment and purification of core fucose glycosylation in patients with schizophrenia have been successfully established and distribution character‐istics of the LCA‐IgG isoforms have been determined ,which could provide a new insight into immune pathomechanism of schizo‐phrenia .
Résumé
Objective To investigate the correlation between the levels of serum immunoglobulin core fucosylation and humoral immune parameters in patients with schizophrenia and its immunological significance .Methods The levels of serum lens culinaris agglutinin(LCA)-IgM ,LCA-IgG ,LCA-IgA ,IgM ,IgG ,IgA ,circular immune complex(CIC) in patients with schizophrenia(n= 46) and health people(n=47) were determined .Their correlations were investigated by linear regression analysis .Results There was a positive correlation between the LCA-IgA and IgG in healthy people(r=0 .311 7 ,P<0 .05) .However ,there was a negative correla-tion between LCA-IgG and IgA in patients with schizophrenia(r=0 .323 6 ,P<0 .05) .In addition ,there were positive correlations between LCA-IgM ,LCA-IgG ,LCA-IgA and CIC(r=0 .354 8 ,r=0 .189 8 ,r=0 .479 7 ,P<0 .05) .Conclusion Increased fucosyla-tion levels of immunoglobulin were detected in patients with schizophrenia ,which may play important roles in immunological inju-ries of central nervous system in schizophrenia .
Résumé
A glycomic method was used to screen the aberrantly ?1-6 fucosylated glycoproteins related to HCC metastasis and analyze the alteration of CK8 both in its expression level and its glycan parts associated with metastatic ability. Based on the approach, 2-DE coupled with lectin affinity blot, lectin affinity precipitation followed by MALDI-TOF-MS/MS, the lens culinaris agglutinin (LCA) affinity glycoprotein profiles from MHCC97-L and MHCC97-H cells, two higher metastatic HCC cell lines, were obtained, in which a differentially displayed protein spot was indicated when compared with Hep3B, in the region within 55～60 ku in molecular mass and 4～6 in isoelectric point. The identification result was CK8 by MALDI-TOF-MS/MS. To confirm the relation between increased core-fucosylation of CK8 and HCC metastasis, LCA affinity precipitation was used to extract the ?1-6 fucosylated glycoproteins, followed by Western blot. And it was found that CK8 was highly fucosylated in both MHCC97-L and MHCC97-H cells compared to Hep3B. Immunofluorescence analysis and Western blot were used to detect its intracellular localization and its protein expression levels, indicating that CK8 distributed in cytoplasm and increased protein expressions in MHCC97-L and MHCC97-H cell lines. And further lectin binding studies found that CK8 has a high affinity for Con A in both MHCC97-H and Hep3B cells, indicating that CK8 was a glycoprotein with high-mannose type N-glycans. But the amount of the lectin RCA-1 binding to CK8 was greater in MHCC97-H than Hep3B, suggesting that CK8 contained the increased terminal galactose residues ?-1, 4-linked to GlcNAc in MHCC97-H. All the results suggested that the increase of CK8 in its protein expression level, core-fucosylation and terminal gal ?1,4 GlcNAc disaccharides might be related to HCC metastatic ability.
Résumé
Tumor recurrence and metastasis are the major cause for cancer patient death. Aberrant glycosylation is one of the important mechanisms of malignant tumor metastasis. The glycoprotein CD24, which could be a ligand for P-selectin on the surface of activated endothelial cells and platelets, appears to be a new diagnostic marker of tumors and possibly participates in tumor metastasis with unknown pathway. ?1,3 Fucosyltransferase Ⅶ (FucT-Ⅶ) is one of the fucosyltransferases which catalyze the transfer of a fucosyl residue from GDP-?-L-fucose to a sugar acceptor, usually galactose in the sugar chains of glycoproteins or glycolipids. The elevated expression of sialyl Lewis (SLeX), the only carbohydrate product of ?1,3 FucT-Ⅶ , predicts an ominous prognosis and high risk of recurrence and regulates the function of CD24. It is possible that ?1,3 FucT-Ⅶ may enhance the glycosylation of CD24 and thus promote the tumor metastasis.