Résumé
Objective To establish the quality control methods and HPLC fingerprint for Haishengsu Injection,a kind of extractions from Tegillarca granosa.Methods Biuret reaction and ninhydrin reaction were used for identification of the Haishengsu Injection.Nitrogen content in the Haishengsu Injection was determined by N_(2) method(semi-trace method).HPLC was used to establish the fingerprint.Other indices of the product were determined.Results The results of the biuret reaction and ninhydrin reaction were positive.Nitrogen content in the Haishengsu Injection was not less than 0.4mg?mL~(-1).And different batches of the Haishengsu Injection have the same dactylogram diagrams.Conclusion We have established rapid,convenient and accurate methods which is suitable and can be used for quality control of the Haishengsu Injection.
Résumé
Objective To investigate the antitumor effect of Haishengsu injection extracted from marine shellfish. Methods Transplant tumor models of sarcoma 180 (S 180), Ehrlich ascites carcinoma (EAC), and hepatoma (Heps) in mice were established. Different doses of Haishengsu injection were given to the mice and the tumor inhibition rates of Haishengsu injection, life span of the mice were calculated. Results The tumor inhibition rates of haishengsu injection (490～1000mg?kg -1?d -1,iv) were 41.10%～49.08% in mice with S 180 and 36.29%～49.19% in mice with hepatoma,respectively. The same doses of Haishengsu injection prolonged the life spans of EAC-bearing mice by 22.93%～69.98%. Conclusion The haishengsu injection has the antitumor effects on the tumor-bearing mice without evident side effects.