Responses of tomato hybrid cultivars to soil application of humic acid under greenhouse conditions

Abstract Responses of three tomato (Lycopersicon esculentum Mill.) hybrids viz. 'Luanova', 'Savarona' and 'Tessera' to various doses (0, 0.5, 1.0, 1.5 and 2.0 g L-1) of soil application of humic acid (HA) were evaluated in terms of plant growth, productivity and quality of fruits, under greenhouse conditions, during 2011-12 and 2012-13. In addition to improved quality characteristics, higher values for yield and yield components were recorded for tomato fruits harvested from plants of 'Tessera' cultivar compared to those harvested from other hybrids tested, regardless of growing seasons. Plants from 'Luanova' cultivar were, however, earlier in fruiting with higher number of fruits per plant. Soil application of HA presented positive effects on yield and physicochemical quality of tomato fruits irrespective of the dose of HA and the cultivar investigated, in both seasons. Soil application of HA at higher dose 1.5 g L-1 exhibited better results in all the three tomato cultivars tested, in terms of their vegetative and reproductive growth, and significantly improved the physicochemical quality of their fruits. Conclusively, the tomato hybrid 'Tessera' was found suitable for cultivation under greenhouse conditions while soil application of aqueous solution of HA @ 1.5 g L-1 substantially resulted in higher production of quality tomato fruits.

Resumo Respostas de três híbridos de tomate (Lycopersicon esculentum Mill.) viz. 'Luanova', 'Savarona' e 'Tessera' para várias doses (0, 0,5, 1,0, 1,5 e 2,0 g L-1) de aplicação de ácido húmico (AH) no solo foram avaliadas em termos de crescimento da planta, produtividade e qualidade de frutas, em casa de vegetação, durante 2011-12 e 2012-13. Além das características de qualidade melhoradas, valores mais elevados para o rendimento e os componentes do rendimento foram registrados para frutos de tomate colhidos de plantas do cultivar 'Tessera' em comparação com aqueles colhidos de outros híbridos testados, independentemente das estações de cultivo. As plantas do cultivar 'Luanova', no entanto, frutificaram mais cedo com maior número de frutos por planta. A aplicação de AH no solo apresentou efeitos positivos sobre a produtividade e a qualidade físico-química dos frutos de tomate, independentemente da dose de AH e do cultivar investigado, nas duas safras. A aplicação de HA no solo na dose maior de 1,5 g L-1 apresentou melhores resultados nos três cultivares de tomate testados, quanto ao crescimento vegetativo e reprodutivo, e melhorou significativamente a qualidade físico-química dos frutos. Conclusivamente, o híbrido de tomate 'Tessera' foi considerado adequado para cultivo em casa de vegetação, enquanto a aplicação no solo de solução aquosa de HA @ 1,5 g L-1 resultou substancialmente em maior produção de frutos de tomate de qualidade.

Correlation and Path Coefficient Studies of Three Line Rice Hybrids

Thirty-one three-line rice hybrids and three commercial checks were used in the current study for correlation and path coefficient analysis. In Kharif 2021, the experiment was carried out at the TCA Hybrid Rice field in Dholi, Pusa, Bihar, India. The experimental design adopted was a Randomized Complete Block Design (RBD) with three replications that included 18 quantitative attributes. Plant height, number of tillers per plant, number of panicles per plant, leaf length, leaf area, kernel length, kernel width, root fresh weight, root dry weight, spikelet fertility, and test weight showed a positive significant correlation with grain yield per plant. Hence, selection for any one of these characteristics would ultimately bring improvement in grain yield. The traits number of tillers per plant, plant height, root fresh weight, leaf length, no. of panicles per plant, leaf width, kernel length, and root volume revealed a significant direct impact on grain yield per plant. Thus, breeding for these characteristics could end up resulting in a higher grain yield.

New Bivoltine Hybrids of the Silkworm (Bombyx mori L.) Performance at Farmer’s Site

The rise in atmospheric temperature, due to global warming, offers a great challenge to make a successful cocoon harvest, as it not only affects the progression of the larvae but also leads to outbreak of diseases. Thus, silkworm breeds have potential to thrive well under such harsh fluctuating climatic conditions without sacrificing commercial traits of cocoon and silk are in-need. Towards this, new bivoltine breeds developed employing heat shock technology, for the first time, were used to develop four bivoltine double hybrids (NBH1 × FC1, NBH2 x FC1, FC1 x NBH1, and FC1 x NBH2) and two poly hybrids (PM x NBH1 and PM x NBH2) and subjected to evaluation for their performance at the farmer's site. Notably, all four double hybrids distributed to farmers performed very well with substantial cocoon yield. Among them, NBH1 x FC1 and NBH2 x FC1 were outperformed with their high fecundity (675 and 700 eggs/dfl) and cocoon yield of 549 and 228 kg, out of 579 and 225 dfls reared by the farmers respectively. On the other hand, 360 and 248 kg of cocoons were harvested as against a total of 325 dfls and 250 dfls of PM x NBH1 and PM x NBH2 distributed to farmers. Eventually, all the farmers were delighted to rear these new double hybrids and poly hybrids, as it fetched a good price to sell the cocoons. Thus, we suggest all these new double hybrids and poly hybrids shall be used for large-scale silkworm rearing in the field conquering the climate change threat to produce both qualitatively and quantitatively better cocoons and silk that shall protect the wealth of Seri-farmers and silk reelers

Genetic Analysis of Pearl Millet [Pennisetum glaucum (L.) R. Br] Lines for Yield and Yield Contributing Traits

This investigation was conducted to study combining ability along with inheritance of grain yield and its component traits in 36 hybrids of pearl millet which were generated through line × tester mating design using 12 male sterile lines and 3 restorers as parental material at IIMR, Hyderabad during kharif, 2022. These hybrids were evaluated in randomized block design with 3 replications during kharif, 2022 and summer, 2023 at IIMR, Hyderabad. In results, both GCA and SCA variances were found significant for majority of characters. The ratio of GCA and SCA variance indicated the predominance of non-additive gene action for all the characters studied except for panicle length and panicle width. GCA effects revealed that parents like 274A, 269A, 04999A and 260A (female) and 123R (male) were good general combiners for grain yield and some contributing characters. On the basis of SCA effects the crosses namely 252A × 124R, 843-22A × 124R, 843-22A × 132R, 242A × 123R, 264A × 132R and 274A × 123R were identified as superior for grain yield and related traits over the seasons. Therefore, it is advised that these parents and hybrids be used in the creation of fruitful hybrids as well as for population growth.

Agronomic Evaluation of Rice (Oryza sativa L.) Hybrids under Agro- Climatic Conditions of Naini, Prayagraj

This research paper aims to evaluate the agronomic performance of twenty rice hybrid varieties under the specific agro-climatic conditions of Prayagraj, Uttar Pradesh, India. The study focuses on analyzing their growth and yield parameters using a randomized block design replicated thrice. The findings will provide valuable insights into the performance of rice hybrids and assist farmers in selecting suitable varieties for optimal productivity in the region. During Kharif season, of 2022 the field experiment was conducted at Crop Research Farm, Department of Agronomy, Sam Higginbottom Institute of Agriculture, Technology and Sciences (SHUATS), Prayagraj (U.P.). Based on the objectives taken maximum plant height (122.57 cm), number of tillers (15.07), plant dry weight (55.91 g/plant), panicle length (29.00 cm), filled grains (256.17), were recorded significantly higher in the hybrid R-504 as compared to other treatments though grain yield/hill (29.13 g) was recorded significantly higher in the hybrid R-410. This research paper aims to provide an in-depth analysis of agronomic evaluation of rice hybrids. The study is intended to explore the advantages and challenges associated with rice hybrid varieties, with a focus on growth attributes, yield potential and other agronomic traits.

Agronomic Evaluation of Mustard (Brassica juncea l.) Hybrids under Agro-climatic Conditions of Prayagraj (U.P), India: Experimental Investigation

A field experiment was carried out at the Crop Research Farm, Department of Agronomy, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj during Rabi season 2022 on Mustard crop. The experiment was laid out in randomized block design with ten treatments and three replications. The soil of experimental plot was sandy loam in texture, nearly neutral in soil reaction (pH 7.1), low in organic carbon (0.36%), available N (171.48 kg/ha), available P (15.2 kg/ha) and available K (232.5 kg/ha). The maximum plant height (175.97 cm), primary branches (8.85), secondary branches (18.85/plant), plant dry weight (40.97 g/plant) at 100 DAS in hybrid M-155 was recorded. Maximum Crop growth rate (CGR) recorded at 60-80 DAS (10.24 g/m2 /day), number of siliqua/plant (466.27), number of seeds/siliqua (13.63), test weight (5.40 g) and seed yield (2.40 t/ha) were observed in hybrid M-155. In terms of economics, highest gross returns (Rs 130905.7/ha), net returns (Rs 81592.73/ha) and B:C ratio (1.65) were observed in hybrid M-155.

Ploidy Determination in Banana Hybrids through Stomatal Studies and Chloroplast Count

Stomata are probably a viable taxonomy distinguishing feature. The study of stomata characteristics of synthetic banana hybrids and their parentage has been carried out. Ploidy determination is essential in banana breeding programs to understand the genetic makeup of hybrid plants. The purpose of this study was to determine the ploidy level of hybrids in contrast with the properties of plant stomata in synthetic banana hybrids with those of their parent plants. Therefore, by examining components such as stomata location, number of epidermal cells, number of stomata cells, stomata length, width, area, intensity and density, as well as chloroplast count, it is possible to compare and contrast the traits of synthetic banana hybrids and their parentage which have different genomes. The entire mount approach was used to make an incision for the purpose of observing stomata. By characterising the stomata traits of the synthetic hybrids and their parentage, the results were then descriptively and qualitatively assessed. Three different locations on the abaxial of the leaf were taken for the determination. The samples were collected from close to the petiole (R1), middle (R2), and distal end (R3), stomata were observed using the replica method. Results showed that more number of stomata was observed in R2of leaf abaxial. The chloroplast count in pairs of stomatal guard cells from the accessions was also measured. The chloroplast density was determined in pairs of stomatal guard cells from the accessions. Based on morphological descriptions, it was observed that 16.66% of the accessions were diploid, 50% were triploid and 33.33% were tetraploid. When considering the chloroplast count, the accessions were categorized into groups, certain accessions classified as tetraploids, others as triploids, and diploids.

Response of Capsicum (Capsicum annuum L.) Hybrids to Micro-nutrients under Protected Conditions

The field experiment was carried out during rabi season of 2021-22 and 2022 at Vegetable Research Farm of Chandra Shekhar Azad University of Agriculture and Technology, Kanpur to find out the response of capsicum hybrids to micronutrients under protected conditions. The experiment was laid out in Split Plot Design (SPD) with 15 treatment combinations and replicated thrice. The experiment comprised three hybrids viz., Indira, Swarna and Bomby and five levels of micronutrients viz., control (no micronutrients application), soil application of Zn, Fe & B, fertigation of EDTA of Zn & Fe and solubor at 15 days interval, foliar application of vegetable special @ 5g/lit at 15 days interval and foliar application of chelated combo micronutrients @ 1g/lit at 15 days interval. Recommended package of practices for the crop were followed except treatments. Results of the experiment revealed that the hybrid Indira recorded significantly highest plant height (91.30 and 94.89 cm), number of branches (11.87 and 12.30), stem girth (4.31 and 4.60 cm), number of leaves (102.94 and 106.43), internode length (13.82 and 14.01 cm), number of flowers per plant (38.22 and 38.36), fruit set (55.10 and 55.44%), no. of fruits per plant (20.99 and 21.22), fruit weight per plant (3.69 and 3.74 kg) and fruit yield per 1000 m2 (70.36 and 71.35 q) while fruit length (11.45 and 11.71 cm), fruit diameter (10.54 and 10.66 cm), weight of placenta (18.51 and 18.71g) and average weight of fruit (193.31 and 195.90g) were significantly highest in Swarna. In case of micronutrients, foliar application of vegetable special @ 5g/at 15 day recorded significantly highest value of plant height (93.72 and 96.77cm), no. of branches (12.66 and 13.39), stem girth (4.75 and 5.12 cm), no. of leaves (105.52 and 108.68), internode length (14.75 and 15.04cm), no. of flowers per plant (41.34 and 41.95), fruit set (56.66 and 57.45%), no. fruits per plant (20.56 and 20.73), fruit length (11.85 and 12.07cm), fruit diameter (10.62 and 10.80cm), weight of placenta (18.31 and 18.46g), average fruit weight (189.03 and 191.33g), fruit weight per plant (4.12 and 4.17 kg) and fruit yield (78.64 & 79.60q/1000 m2). Hence, hybrid Indira and foliar application of vegetable special @ 5g/at 15 days may be recommended for higher productivity from capsicum crops under protected conditions.

Cultivation of Hybrids Mustard (Brassica juncea L.) in the Pedo- Climatic Conditions of paryagraj Sangam Region (U.P), India

The field experiment was carried out at the Crop Research Farm, Department of Agronomy, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj during Rabi season 2021-22 on Mustard crop. The experiment was performed in randomized block design with ten treatments and three replication. The soil of experimental plot was sandy loam in texture, nearly neutral in soil reaction (pH 7.1), low in organic carbon (0.36%), available N (171.48 kg/ha), available P (15.2 kg/ha) and available K (232.5 kg/ha). The maximum plant height (171.76 cm), primary branches (6.5/plant), secondary branches (16.20/plant), plant dry weight (43.43 g/plant) at 100 DAS in hybrid ADV 4029 was recorded. Maximum Crop growth rate (CGR) recorded at 40-60DAS (20.32 g/m2 /day), number of siliqua/plant (457.27), number of seeds/siliqua (13.55), test weight (4.73 g) and seed yield (2.51 t/ha) were observed in hybrid ADV 4029. In terms of economics, highest gross returns (Rs 136660.90/ha), net returns (Rs 87347.93/ha) and B:C ratio (1.77) were observed in hybrid ADV 4029.

Regression Equation Model for Brinjal Pests (Solanum melongena L.) in Maharashtra Plain Zone

An experiment “Effect of weather parameters on growth, yield and insect pest infestation on brinjal varieties under different planting windows” was carried out at Faculty of Agriculture Department of Agricultural Meteorology Farm, Centre for Advanced Agricultural Meteorology, College of Agriculture, Pune during Kharif seasons of 2014 and 2015. The experiment was laid out in split plot design with three replications. The treatment comprised of three brinjal hybrids viz., V1:Phule Arjun, V2: Krishna, V3: Panchganaga as main plot and four planting windows viz., P1: 31st MW (30 July-5 August), P2: 32ndMW (6-12 August), P3:33rdMW (13-19 August) and P4: 34thMW (20-26 August) as sub plot treatments.The correlation of meteorological parameters with incidence of brinjal shoot and fruit borer was studied in 2014. The correlation of shoot and fruit borer on brinjal (0.764*and 0.796*) and (0.784* and 0.704*) with the weather parameters are presented. Whereas morning relative humidity, rainfall and bright sunshine hours showed positive correlation with shoot and fruit borer population Average number of aphids/ jassid/ whiteflies plant showed highly significant negative correlation with minimum temperature(r= -0.702*,-0.710* and 0-.800*,) respectively. During 31stMW planting windows maximum temperature showed significant negative correlation with shoot and fruit borer population(r= -0.796*).It was observed that the infestation of shoot and fruit borer started increasing from October to November and its decreased onwards meteorological week. Amongst all the brinjal hybrids, Phule Arjun hybrids found significantly superior under extended planting windows followed by Krishna and Panchganga. Planting during 31st MW (1st week of August) was observed to be most suitable and optimum for brinjal considering the growth and yield attributes. This planting window was at par with 32nd MW planting window. Linear correlation analysis for brinjal fruit yield with weather parameters was significantly positively correlated with maximum temperature and minimum temperature.Timely planting during 31st MW (P1) and 32nd (P2) recorded lower incidence of shoot and fruit borer, aphids, jassid and whiteflies. Whereas, crop planted during 34thMW (P4) recorded maximum incidence of all pests. Among the brinjal hybrids, higher incidence was recorded with Panchganaga and minimum was recorded on Phule Arjun.Among the brinjal hybrids lower incidence of shoot and fruit borer, aphids, jassids and whiteflies was recorded on hy. Phule Arjun, which was found to be tolerant. This was followed by hy. Krishna. The higher incidence of all pests was recorded hy. Panchganga, which was found to be susceptible. Pest population on brinjal shoot/fruit/Aphid/Jassid/Whiteflies had significant negative correlation with minimum temperatures, whereas, morning and evening relative humidity, wind speed, pan evaporation and bright sunshine hours showed positive correlation pests population.

Detection of True Hybrids in Pearl Millet Cross Combinations by Employing SSR Molecular Markers

Pearl millet (Pennisetum glaucum L. R. Br.), a crucial staple food and significant cereal crop, is gaining prominence due to its versatile applications as feed, food, and fodder. Heterosis of this crop has been extensively harnessed to increase productivity. Hybrid variants exhibit superior grain and stover yields compared to open-pollinated varieties.Top of Form The primary aim of this investigation was to evaluate and confirm authentic hybrids within three resultant F1 progenies. The assessment of parents and F1 hybrids was carried out during the Kharif 2021for the purpose of accurate discrimination and rapid verification of true hybrids by employing 20 SSR molecular markers. The experimental materials consisted of three distinct cytoplasmic male sterile (CMS) lines including ICMA 843-22, ICMA 04999, and ICMA 02333, used as female parents along with three fertility restorers, viz., ICMR 01004, ICMR 20233, and ICMR 20342, were utilized as male parents. The analysis of SSR profiles was based on distinctive banding patterns, resulting in unique profiles for the hybrids. The amplified fragment sizes ranged between 90 to 300 base pairs (bp), effectively enabling the differentiation of authentic hybrids. Within the specific crosses, the percentage of polymorphism was determined 75% for the cross ICMA 843-22 × ICMR 01004, 80% for the cross combination ICMA 04999 × ICMR 20233, and 75% for the cross ICMA 02333 × ICMR 20342. The true hybrids were calculated using hybrid purity percentage formula using heterozygous banding patten among total plants evaluated. Among a total of 100 F1 plants, 85, 86, and 88 plants were accurately identified as true hybrids in the respective crosses i.e., ICMA 843-22 × ICMR 01004, ICMA 04999 × ICMR 20233, and ICMA 02333 × ICMR 20342. The identified markers hold significant potential for applications such as hybridity test, genetic purity assessments, diverse germplasm identification, and DNA fingerprinting endeavors in future.

Effect of Phosphorus Levels on Growth and Yield of Maize Hybrids

A field experiment was conducted at the Crop Research Farm, Department of Agronomy, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj during Rabi season 2022 on Maize crop. The experiment was laid out in Randomized Block Design with ten treatments and three replication. The treatment consisted of Maize hybrids (Govinda 111, Hybrid corn 4226, DKC 9133) with soil application of phosphorus (40 kg/ha, 60 kg/ha and 80 kg/ha) and a control (120:60:40 kg NPK/ha). The results of the experiment showed that, plant height (125.65 cm), dry weight (96.59 g/plant), crop growth rate (39.91 g/m2/day), No. of cob/plant (1.33), No. of grains/row (21.36), seed index (23.44 g), grain yield (5.90 t/ha) and stover yield (9.91 t/ha) and harvest index (37.12%) were significantly highest recorded in ‘Hybrid DKC 9133’ with the application of phosphorus 80 kg/ha. Maximum gross returns (1,32,865.00 INR/ha), net returns (89,392.55 INR/ha), and B:C (2.06) were also obtained with the same treatment.

Effect of Micrometeorological Parameters on Growth and Yield of Brinjal (Solanum melongena L.) under Different Planting Windows

An experiment was carried out at Faculty of Agriculture Department of Agricultural Meteorology Farm, Centre for Advanced Agricultural Meteorology, College of Agriculture, Pune during Kharif seasons of 2014 and 2015.The experiment was laid out in split plot design with three replications. The treatment comprised of three brinjal hybrids viz.,V1:Phule Arjun, V2: Krishna, V3: Panchganaga as main plot and four planting windows viz., P1: 31st MW (30 July-5 August), P2: 32ndMW (6-12August), P3:33rdMW (13-19 August) and P4: 34thMW (20-26 August) as sub plot treatments. In micrometeorological studies of the higher radiation absorptions and lower reflection was absorbed under hy. Phule Arjun as compared to hy. Krishana and hy Panchganga. The maximum Incident PAR (1270 u mol m-2s-1) Intercepted PAR (86.47u mol m-2s-1),Absorbed PAR (1094.4u mol m-2s-1) and Radiation use efficiency (2.43gmMJ m-2) was observed in hy .Phule Arjun. Cumulative GDD, HTU and PTU at the end of each growth stages showed that numerically higher requirement was observed in hy. Phule Arjun over hy. Krishana and hy. Panchganaga hybrids during both year 2014 and 2015 experimentation period. Whereas, the lowest canopy temperature was found in hy. Phule Arjun (29.0 0C) than rest of the brinjal hybrids. Canopy reflected PAR and transmitted PAR was higher in (191.54 and 188.62 µ mol m-2s-1) Panchganaga hybrids among the brinjal hybrids.

Evaluation of contact angle and mechanical properties of resin monomers filled with graphene oxide nanofibers

Abstract This in vitro study synthesized hybrid nanofibers embedded in graphene oxide (GO) and incorporated them into experimental resin composite monomers to evaluate their physical-mechanical properties. Inorganic-organic hybrid nanofibers were produced with precursor solutions of 1% wt. GO-filled Poly (d,l-lactide, PLA) fibers and scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) characterized the morphology and chemical composition of the spun fibers. Resin composite monomers were developed and a total of 5% nanofibers were incorporated into the experimental materials. Three groups were developed: G1 (control resin monomers), G2 (resin monomers/PLA nanofibers), and G3 (resin monomers/inorganic-organic hybrid nanofibers). Contact angle (n=3), flexural strength (n=22), elastic modulus (n=22), and Knoop hardness (n=6) were evaluated. The mean of the three indentations was obtained for each sample. The normality of data was assessed by QQ Plot with simulated envelopes and analyzed by Welch's method (p<0.05). Overall, SEM images showed the regular shape of nanofibers but were non-aligned. Compositional analysis from EDS (n=6) revealed the presence of carbon and oxygen (present in GO composition) and Si from the functionalization process. The results of contact angle (°) and hardness (Kg/mm2) for each group were as follow, respectively: G1 (59.65±2.90; 37.48±1.86a), G2 (67.99±3.93; 50.56±1.03b) and G3 (62.52±7.40; 67.83±1.01c). The group G3 showed the highest Knoop hardness values (67.83 kg/mm2), and the flexural strength of all groups was adversely affected. The experimental resin composite composed of hybrid nanofibers with GO presented increased hardness values and hydrophilic behavior.

Resumo Este estudo in vitro sintetizou nanofibras híbridas embebidas em óxido de grafeno (GO), incorporando-as à uma resina composta experimental de monômeros para avaliar suas propriedades físico-mecânicas. Nanofibras híbridas inorgânica-orgânicas foram produzidas com soluções precursoras de fibras poli (d, l-lactídeo, PLA) preenchidas com GO a 1% em peso e microscopia eletrônica de varredura (MEV) e espectroscopia de raio-X de energia dispersiva (EDS) caracterizaram a morfologia e composição química das fibras. Monômeros de resina composta foram desenvolvidos e um total de 5% de nanofibras foi incorporado aos materiais experimentais. Três grupos foram desenvolvidos: G1 (monômeros de resina controle), G2 (monômeros de resina/ nanofibras de PLA) e G3 (monômeros de resina/nanofibras híbridas inorgânico-orgânicas). Ângulo de contato (n=3), resistência à flexão (n=22), módulo de elasticidade (n=22) e dureza Knoop (n=6) foram avaliados. A média das três endentações foi obtida para cada amostra. A normalidade dos dados foi avaliada pelo QQ Plot com envelopes simulados e analisada pelo método de Welch (p<0,05). No geral, as imagens de MEV mostraram forma regular de nanofibras, mas não alinhadas. A análise composicional de EDS (n=6) revelou a presença de carbono e oxigênio (presentes na composição do GO) e Si resultante do processo de funcionalização. Os resultados do ângulo de contato (°) e dureza (Kg/mm2) para cada grupo foram os seguintes, respectivamente: G1 (59,65±2,90; 37,48±1,86a), G2 (67,99±3,93; 50,56±1,03b) e G3 (62,52±7,40; 67,83±1,01c). G3 apresentou os maiores valores de dureza Knoop (67,83 kg/mm2), e a resistência à flexão de todos os grupos foi prejudicada. A resina composta experimental composta por nanofibras híbridas com GO apresentou maiores valores de dureza e comportamento hidrofílico.

Heterosis Analysis Over Environments for Seed Yield and Its Attributing Traits in Linseed (Linum usitatissimum L.)

A complete set of 79 entries comprising of twelve parents, their 66 F1s and one check RLC-92 were evaluated during rabi 2020-21 at two locations i.e., ARS, Dahod, and BTRS, Anand, under two dates of sowing i.e., 2nd fortnight of October and 1st fortnight of November. The present study aimed to investigate the Heterosis analysis over environments for seed yield and its attributing traits in linseed (Linum usitatissimum L.). Among the all four environments, E1 had higher mean value for seed yield per plant and all other important yield contributing traits indicating sowing in second fortnight at Dahod conditions best for linseed cultivation. The hybrids viz., Indira × ILS 264, RLC 133 × Kartika, H 45 × Kartika, IPI 10 × H 45 and IPI 10 × Kartika depicted higher better parent heterosis (HB) and Shekhar × GS 384 and Shekhar × H 45 noted significant standard heterosis for seed yield per plant and other traits. Parental lines, Shekhar, GS 384, H 45, KB 961, IPI 10 and Kartika yielded superior heterotic crosses for seed yield and its component characters. The heterotic effect for seed yield per plant was found to be associated with heterosis for its related traits in the majority of the crosses. High standard heterosis and heterobeltiosis for seed yield per plant and its component characters suggested ample scope of exploiting heterosis commercially. The magnitude of heterosis provides information on the extent of genetic diversity in parents of a cross and helps in choosing the parents for superior Fl’s, and to exploit hybrid vigour.

Desenvolvimento de inibidores duais de histona desacetilase 6 (HDAC6) e tirosina quinase (TQ) com vistas ao tratamento do câncer / Design of histone deacetylase 6 (HDAC6) and tyrosine kinase (TK) dual inhibitors for cancer treatment

Câncer é a denominação atribuída a um conjunto de doenças que são responsáveis pela segunda maior causa de morte no Brasil e no mundo. A quimioterapia figura entre uma das estratégias utilizadas para o tratamento e cura do câncer, sendo amplamente empregada em estratégias terapêuticas isoladas, ou em associação à radioterapia e cirurgia. A enzima histona desacetilase 6 (HDAC6) é responsável por desacetilar a cadeia lateral de N-acetillisinas em -tubulinas, desempanhando papel crítico na dinâmica do citoesqueleto celular, estando superexpressa em uma série de neoplasias. Neste sentido, na última década os receptores tirosina quinase (TQ) foram os principais alvos de fármacos aprovados para o tratamento do câncer e de doenças autoimunes e continuam atraindo a atenção de grupos de pesquisa dada a exorbitante diversidade do quinoma humano. É sabido que a monoterapia seja com inibidores de HDAC, seja com inibidores TQ, apresenta problemas de toxicidade, reações adversas, ineficácia, resistência e/ou recidiva. Diversos estudos relatam o desenvolvimento de inibidores duais de HDAC-TQ, almejando tanto a simplificação do tratamento, quanto sinergismo terapêutico e redução de efeitos adversos. Assim, o presente trabalho apresenta o planejamento, síntese e avaliação da citotoxicidade de inibidores duais, potencialmente seletivos para HDAC6 e receptores TQ. No total, 23 compostos foram sintetizados entre 2 a 4 etapas. Todos os compostos finais foram caracterizados por RMN (1H e 13C) e espectrometria de massas de alta resolução (HRMS). A citotoxicidade foi determinada pelo ensaio de MTT, em linhagens derivadas de tumores sólidos (HCT116 e MCF-7) e hematológicos (Jurkat e Namalwa). Os compostos apresentaram citotoxicidade em concentrações micro e nanomolares em todas as linhagens testadas, sendo que a linhagem MCF-7 foi a mais resistente à ação dos compostos, e as linhagens hematológicas foram as mais sensíveis. Os inibidores 4d-f foram os mais ativos na triagem por MTT, com IC50 iguais a 20, 30 e 50 nM, respectivamente, em células Jurkat. Estudos mecanísticos do efeito citotóxico indicaram que os compostos 4d-f exercem atividade de forma tempo-dependente, e majoritariamente por ação antiproliferativa, embora estímulos apoptóticos também tenham sido observados nos estudos. Simulações de ancoramento molecular (docking) e de relação entre as estruturas químicas dos compostos e suas respectivas atividades biológicas (REA) permitiram identificar padrões moleculares, propriedades físico-químicas e eletrônicas que potencialmente possuem relação com a atividade biológica dos compostos, permitindo futuras otimizações do arcabouço molecular desta série de compostos. Tomados em conjunto, os resultados deste trabalho revelam o potencial terapêutico de inibidores duais de HDAC6-TQ. Notadamente, os compostos apresentados aqui podem ser os primeiros potenciais inibidores duais de HDAC6-TQ a serem reportados na literatura

Cancer is the name of a series of diseases that are the second main cause of death in Brazil and worldwide. Chemotherapy is one of the main strategies to treat and cure cancer, and has been widely applied as a single therapeutic agent, and in association with radiotherapy and surgery. Histone deacetylase 6 (HDAC6) deacetylates N-acetyllysine side chains of tubulin, playing crucial role on cytoskeletal dynamics, and could be overexpressed in several cancers. Tyrosine kinase receptors (TK) have been the main targets of FDA-approved drugs through the last decade for both cancer and autoimmune diseases, and have been attracting special attention of research groups due to the exorbitant diversity of the human kinome. It is known that either HDAC or TK single therapy have toxicity issues, adverse effects, inefficacy, resistance and/or recidive. Therefore, many studies report the design of HDAC-TK dual inhibitors aiming simpler treatments, synergism of action and side effects reduction. Herein, the design, synthesis and cytotoxic evaluation of dual and selective HDAC6-TK inhibitors are presented. A total of 23 compounds were designed and synthesized through 2 to 4 steps. All final compounds were characterized by 1H/13C NMR and high-resolution mass spectrometry (HRMS). The cytotoxicity of compounds was determined by MTT assay for both solid (HCT116 and MCF-7 cells) and hematological cancers (Jurkat and Namalwa cells). Compounds exhibited micro and nanomolar ranges of cytotoxicity for all cell lines tested. MCF-7 cells were the most resistant against the treatment, and hematological cells were more susceptible to the cytotoxic effect of the compounds. Compounds 4d-f were the most actives in the MTT screening against Jurkat cells (IC50 = 20, 30 and 50 nM, respectively). Mechanistic studies regarding the cytotoxic effects of 4d-f indicated that the compounds induced cell death in a time-dependent manner mainly via cytostatic activity even though apoptotic stimuli were observed also. Molecular docking and structure-activity relationships (SARs) allowed the identification of molecular patterns, and physicochemical and electronic properties that potentially modulate the biological activity of these compounds, allowing further optimizations of the molecular scaffold for these series of compounds. Taken together, the results of this study reveal the therapeutic potential of HDAC6-TK dual inhibitors. Noteworthy, the compounds reported herein could be the first HDAC6-TK dual inhibitors ever reported in literature

Nursery performance of potentially promising rootstocks for citriculture in the south of Brazil / Desempenho em viveiro de porta-enxertos potencialmente promissores para a citricultura do sul do Brasil

ABSTRACT: Diversification of rootstock varieties, with consequent reduction in phytosanitary risks, has great importance to the sustainability of citrus cultivation in the south of Brazil. This study evaluated the performance of 42 rootstocks in the nursery phase to generate 'Valencia Late' sweet orange seedlings. Therefore, nucellar seedlings from the rootstocks under study were analyzed in relation to plant height, stem diameter, mortality rate and percentage of bud set after grafting. The experimental design was completely randomized with four replications of 20 sample units. Not adapted genotypes had high mortality rates, and the ones of both hybrids LCR x CTSW - 009 and LVK x LCR - 038 were 100% and 90%, respectively. Other 13 genotypes had mortality rates above 30%, and 'Sunki' mandarin was the female genitor of ten of them. 'Swingle' citrumelo was the rootstock with the highest development (plant height and stem diameter), associated with low mortality rate. In relation to the other rootstocks, the best results were the fast grafting diameter, associated with low mortality rate obtained by hybrids HTR - 053, LRF x (LCR x TR) - 005, CLEO x TRBN - 245, CLEO x TRSW - 287, and citrandarins 'Indio', 'Riverside' and 'San Diego', as well as Trifoliate orange and 'Rangpur' lime. In addition to Trifoliate orange, which is widely used in Rio Grande do Sul, these rootstocks have great potential in citriculture in the south of Brazil.

RESUMO: A diversificação do uso de variedades porta-enxerto, com a consequente redução de riscos fitossanitários, é de fundamental importância para a sustentabilidade da citricultura do Sul do Brasil. O objetivo deste trabalho foi avaliar o comportamento de 42 porta-enxertos na formação de mudas de laranjeira 'Valência Late' em fase de viveiro. Nesse sentido, seedlings (plantas oriundas da germinação de sementes) nucelares dos porta-enxertos estudados foram avaliados relativamente aos caracteres altura da planta, diâmetro do caule, taxa de mortalidade e percentagem de pegamento de borbulhas após a enxertia. O delineamento experimental foi inteiramente casualizado, com quatro repetições de 20 unidades amostrais. Genótipos não adaptados tiveram altas taxas de mortalidade, os híbridos LCR x CTSW - 009 e LVK x LCR - 038 apresentaram 100% e 90% de mortalidade, respectivamente. Mais 13 genótipos apresentaram mortalidade superior a 30%, destes, dez têm como parental feminino a tangerineira 'Sunki'. O citrumelo 'Swingle' foi o porta-enxerto que apresentou maior desenvolvimento (altura da planta e diâmetro do caule), associado a uma baixa taxa de mortalidade. Quanto aos demais porta-enxertos, destacaram-se por atingir rapidamente o diâmetro de enxertia, associado a baixa taxa de mortalidade, os híbridos HTR - 053, LRF x (LCR x TR) - 005, CLEO x TRBN - 245, CLEO x TRSW - 287, citrandarins 'Indio', 'Riverside' e 'San Diego', assim como o limoeiro 'Cravo' e o Trifoliata. Além do Trifoliata, amplamente utilizado no Rio Grande do Sul, estes porta-enxertos são promissores para a citricultura do Sul do Brasil.

Autoatención y automedicación: reflexiones y retos desde la ontología del ser social / Self-care and Self-medication: Reflections and Challenges from the Ontology of a Social Being

Resumen La autoatención, aunque la mayor parte del tiempo se considera como un problema de salud pública, también puede ser considerada un acto de empoderamiento y contrahegemonía; sobretodo, si se toma en consideración la autonomía de los individuos para aliviar y comprender una enfermedad o padecimiento. En el contexto institucional de la atención de la salud, la autoatención se convierte en un espacio donde conviven diversos tipos de instituciones, saberes y representaciones. Por lo anterior, los actores sociales que practican autoatención resignifican y descentralizan la práctica médica y el conocimiento científico. Una parte de esas prácticas es la automedicación. El objetivo de este artículo es explicar cómo la autoatención y la automedicación son parte de la vida cotidiana de los individuos, y cómo esta práctica no se limita a un ámbito médico; en cambio, propone ubicar la práctica de autoatención como parte de una discusión más amplia que tiene base en la ontología del ser social expresada en categorías como el hogar y lo cotidiano. Se plantean retos que requieren pensar en la autoatención y la automedicación desde un pluralismo disciplinar que va desde la antropología y la sociología médica, hasta la economía de la salud, la psicología y la farmacología. Se reflexiona en el hogar como primer espacio real de atención, donde se expresan prácticas laicas y tácticas microsubersivas; el cotidiano como un reflejo de aspectos históricos, sociales, coyunturales, políticos, ambientales, entre otros. Se plantea un modelo atención más allá del consultorio, lo vestal o privado, trasciende la idea del antiguo consultorio.

Abstract Self-care, although most of the time is seen as a public health problem, may also be considered an act of empowerment and counter-hegemony if it takes into consideration the autonomy of social groups to self-understand and self-alleviate a disease or illness. When it comes to the institutional health care context, self-care becomes a space where various types of institutions, knowledge, and representations coexist. Due to the foregoing, social actors practicing self-care re-signify and decentralize medical practice and scientific knowledge, by means of their practice of self-medication. The objective of this article is to explain how selfcare and self-medication are part of an individual's everyday life, and how this practice is not limited to the medical realm; instead, it proposes to locate self-care practices as part of larger discussion about the ontology of a social being, expressed in categories such as the home and the everyday. But doing that poses a series of challenges that require thinking about self-care and self-medication from a perspective of disciplinary pluralism, ranging from anthropology and medical sociology to health economics, psychology and pharmacology. Home is reflected as the first real space of attention, where secular practices and micro-subversive tactics are expressed; also, everyday life as a reflection of historical, social, political and environmental aspects, among others. A care model is proposed beyond the office and the private, transcending the classical idea of a doctor's office.

Synthesis of ureasil-polyether film forming materials by using environmentally friendly solvent

Organic inorganic hybrids present several advantages as drug release systems, such as: high flexibility, high mechanical and thermal resistance, transparency, and low water solubility. These hybrids are synthesized through a chemical route named sol-gel that usually uses as solvente tetrahydrofuran (THF). Objetives: To develop film formers from hybrid materials replacing THF with ethanol, a less toxic solvent for skin application and for the environment. Methods: Four polymers were used: two based on polyethylene oxide (PEO) with molecular weight of 500 and 1900 g mol-1 and two based on polypropylene oxide (PPO), with molecular weight of 400 and 2000 g mol-1. The structural analysis was performed by FTIR, 1H-NMR and 29Si-NMR, and the thermal-mechanical analysis by DSC and TG-DTA. Results: The results of the thermo-mechanical analysis revealed that the solvent replacement did not affect the thermal stability and flexibility of the di-ureasil hybrid. Conclusions: Structural characterization confirmed the formation of hybrids both in THF and in ethanol. Therefore, ethanol is an excellent solvent for the synthesis of these hybrid matrices, since it allows obtaining the same material without changing its characteristics, with some advantages, however, over THF. Furthermore, this paper describes the efficiency of ethanol as a solvent, which is environmentally friendly, to replace THF in the physical-chemical characteristics of these filming former materials.

Coffea canephora breeding: estimated and achieved gains from selection in the Western Amazon, Brazil / Melhoramento do Coffea canephora: ganhos de seleção estimados e realizados na Amazônia Ocidental

ABSTRACT: Gain from selection is an important criterion in quantifying the efficiency of breeding programs. This study quantified the selection gain estimated under experimental conditions and realized gain achieved in the field, seeking to interpret the efficiency of the Coffea canephora selection. For that purpose, we considered experiments that began in 2004 with directed hybridizations to obtain new hybrid progenies. From a breeding population composed of 288 hybrid individuals, 12 genotypes were selected in experimental conditions from 2005 to 2012, with amplitude in the estimated gains from 127.70 to−19.19%. Those genotypes were evaluated from 2012 to 2018 in clonal tests in four environments of the Western Amazon. The environment that exhibited the greatest correlation between the predicted genetic values and the realized genetic gain observed in the field was the environment of Ouro Preto do Oeste, RO (0.67), the location in which the plants were selected, followed by the environments of Alta FlorestaD´Oeste, RO (0.44), Rio Branco, AC (0.43), and Porto Velho, RO (0.37).Experimental conditions showed that the effect due to dominance deviations was approximately three times greater than the additive effect. Nine clones exhibited higher genetic gains in the experimental conditions and at field, and two clones exhibited lower estimated gains and lower field performance.The clone G17-P7 exhibited high genetic gain under experimental conditions and low field performance. The selection in experimental conditions was positively correlated with plant performance in the field (r=0.55), which allows reduction of the original breeding population to a set of more promising clones to be grown in multiple environments, optimizing time and resources.

RESUMO: O ganho com a seleção é um dos critérios mais importantes para avaliar a eficiência de programas de melhoramento de plantas. O objetivo desse trabalho é quantificar a associação entre o ganho de seleção estimado em condições experimentais e o ganho de seleção realizado em campo, buscando quantificar a eficiência do melhoramento do Coffea canephora. Para isso, foram considerados experimentos iniciados com a hibridação direcionada para obtenção de progênies no ano de 2004, a avaliação de testes de progênies em condições experimentais no período de 2005 a 2012, e avaliações em diferentes ambientes da Amazônia Ocidental no período de 2012 a 2018. De uma população composta por 288 indivíduos estruturados em nove progênies de irmãos completos foram selecionados 12 genótipos com amplitude de 127,70 a -19,19% em suas estimativas de ganho com a seleção, para serem avaliados em quatro ambientes da Amazônia Ocidental. O ambiente que apresentou maior correlação entre os valores genéticos preditos e o ganho realizado foi Ouro Preto do Oeste - RO (0,67), local em que as plantas foram selecionadas, seguido pelos ambientes de Alta Floresta D´Oeste - RO (0,44), Rio Branco - AC (0,43) e Porto Velho - RO (0,37). Avaliações em condições experimentais mostraram que os efeitos devido aos desvios de dominância foram aproximadamente três vezes maiores do que os efeitos aditivos. Nove clones apresentaram maiores estimativas de ganho em condições experimentais e nos ensaios de campo, e dois clones apresentaram menores estimativas em ambas condições. O clone G17-P7 apresentou altas estimativas de ganho em condições experimentais e baixo desempenho em campo. A seleção em único ambiente esteve positivamente correlacionada com o desempenho dos cafeeiros em campo (r=0,55), permitindo reduzir a população de melhoramento original em um conjunto de clones de maior potencial agronômico.