Isolation and Characterization of Microbes of Different Kinds of Street Food and Determination of Antibiotic Susceptibility of the Isolates

Aim: Improper personal hygiene can facilitate the transmission of the pathogenic microorganisms found in environment and on people抯 hands via food to humans. The present study was undertaken to investigate the microbiological quality of different street food and determining the antibiotic susceptibility of the isolated microorganisms. Study Design: Collection of food samples for isolation of Pathogenic Microorganisms, to identify them by using Biochemical test, molecular test (16sr RNA typing), Antibiotic susceptibility was done by using different antibiotics against the isolates. Place and Duration of Study: Food samples were collected from street vendors of Sainkpuri area, work was done from December 2020 to April 2021 at Microbiology Department, St. Francis College for Women, Hyderabad. Methodology: Five samples of street food were collected in sterilized bottles, tested for the presence of microorganisms by following standard microbiological method used for isolation of microorganisms. The organisms were identified by carrying out various biochemical test according to Bergey抯 Manual of Systematic Bacteriology. The Molecular characterization was done based on 16sr RNA typing. Determining the sensitivity of the isolates against different Antibiotics by employing Kirby Bauer technique. Results: The organisms isolated from Manchuria and Ragada samples: Lactobacillus delbrueckii; Lactobacillus casei, Klebsiella pneumonia, Enterococcus faecalis and Vibrio spp respectively; Samosa and bonda: Staphylococcus aureus, Lactobacillus delbrueckii and Pantoea dispersa; Pani puri water: Staphylococcus aureus and Providencia vermicola. Antibiotic susceptibility tests showed that most of the isolated microorganisms were sensitive to Ciproflaxin. Conclusion: Hence the quality of street food is found to be low due to following factor: lack hygiene conditions in the food preparation, ingredients may also affect quality of the food, and presence of air borne microorganisms in the surrounding areas of service points.

A Survey Report on Ikki-Ryo : A Moxibustion Clinic Affiliated with the Uchihara Training Camp (1938-1945) for the Japanese Youth Volunteer Brigade for Reclamation of Manchuria and Mongolia / 日本東洋医学雑誌

Ikki-Ryo was a moxibustion clinic established as part of a national project facility that belonged to the Uchihara Training Camp (1938-1945) for the Japanese Youth Volunteer Brigade for Reclamation of Manchuria and Mongolia. Since such a case is extremely rare in the modern medical history of Japan, this article summarizes the information on Ikki-Ryo obtained through documents collection and analysis, interviews, and fieldwork investigation. Ikki-Ryo was proposed by acupuncturists Bunshi Shirota and Kyohei Tanaka, and was established under the directorship of Kanji Kato. It played a role in the clinical practice and training of moxibustion techniques. The data suggests that Ikki-Ryo provided advantageous clinical effects in the promotion of good health and in the treatment of some diseases (e.g. enuresis, suspected tuberculosis) for the trainees, while it is indicated that this clinic had a certain geographical, organizational, and emotional distance from other medical or healthcare departments at the camp. Although the role of moxibustion has changed throughout history, the concept and activities of Ikki-Ryo are still thought-provoking from the perspective of the diversity, versatility, complementarity, and sustainability of healthcare tools.

The First and the Second Pneumonic Plague in Manchuria and the Preventive Measure of Japanese Colonial Authorities (1910-1921) / 의사학

During the first plague epidemic in Manchuria (1910-1911), Japanese Government-General in Korea had not reported a plague patient at all in official. This did not mean the preventive measure of colonial authorities was successful. Their prevention program and measure were operated inadequately. They focused on instigative and sometimes irrelevant aspects such as rat removal to restore order in the colony. The quarantine facility was insufficient so that some people could not be effectively isolated. The reason pneumonic plague did not spread from Manchuria to Korea was mostly because Chinese coolie did not enter Korea. The colonial government promulgated Jeonyeombyeong Yebangryeong (Preventive Regulation of Contagious Disease) in June 5, 1915. This regulation aimed at unitary control by police and was strengthened 10-day quarantine. After the March First Movement, the colonial government tried to change imperial policy to cultural policy. The military police and civilian police were bifurcated and governors took charge of health administration. However, sanitary police still played important role for preventive measure. The preventive policy of colonial government experienced important change from cholera epidemic between 1919 and 1920. The death toll of two years had exceeded 20,000 people. During the cholera outbreak of two years, quarantine and isolation were emerged as important tools to prevent disease transmission, and were well-appointed more now than before. To prevent cholera epidemic, the colonial government strengthened house-to-house inspection as well as seaport quarantine, train quarantine, passenger quarantine. House-to-house inspection detected sixty percentage of cholera patients. When the second Manchurian plague spread in Korea in 1920-1921, this plague was known to Korean people as pneumonic plague. The colonial government propagated and educated pneumonic plague, and urged to wear a mask through Heuksabyeong Yebang Simdeuk (The Notandum for Plague Prevention). The colonial government did not focused on rat removal any more. They pointed out Chinese coolie as a source of infection. Though they did not know exact information and analysis on pneumonic plague, the preventive measure of the second plague in Manchuria was successful due to the well-equipped disinfection system such as house-to-house inspection and nationwide quarantine made by cholera prevention. While the first prevention of plague in Manchuria was successful despite rubbish preventive system, the second prevention of plague in Manchuria was successful due to the well-equipped disinfection system in spite of occurring plague patients.

Suh Yang Park, who Became a Doctor as a Son of a Butcher / 의사학

Dr. Suh Yang Park was born in 1885 as a son of a butcher, which was the lowest class at that time in Korea. However, contact with western missionaries, including Dr. O. R. Avison, provided him with an understanding of western civilization. After entering Chejungwon Medical School in 1900, Dr. Park learned basic sciences, basic medical sciences like anatomy and physiology, and other Western medical specialties such as internal medicine and surgery. He graduated from medical school in 1908 and received Government Certificate from Home Office, the first in Korea in this field, which granted the right to practice medicine. His certificate number was 4th overall. As both a doctor and a talented musician, Dr. Park actively participated in the social enlightenment movement. He was quite progressive in his time, having surpassed the social limitations as a son of a butcher, as well as actively propagating his knowledge of Western civilization onto others. After graduation, he had served as a professor at the school he graduated from, until he went into exile in Manchuria in 1917 due to the annexation of Korea by Japan in 1910. There, he opened a hospital and provided medical treatment for Koreans. He also established a school for young Koreans, inspiring them with a sense of nationalism. Also, Dr. Park was an active member of various Independence Movement Organizations in Manchuria. Then in 1932, at the time when Japan took control of Manchuria, his school was closed down. As a result, Dr. Park couldn't help but stop his anti-Japanese activities. In 1936, he returned to his homeland and passed away in 1940, just five years before the liberation of Korea from Japanese occupation.