Effects of miR-1290 mediated by ultrasound microbubbles on proliferation, apoptosis and invasion of ovarian cancer cells by regulating the expression of DKK3 / 中华内分泌外科杂志

Objective:To investigate the mechanism of miR-1290 mediated by ultrasound microbubbles on the proliferation, apoptosis and invasion of ovarian cancer cells by regulating the expression of DKK3.Methods:Logarithmic SKOV3 cells were divided into Control group, miR-1290 NC group, microbubble treatment (MB) group, miR-1290 inhibitor group and miR-1290 inhibitor-MB group. The targeting relationship between miR-1290 and DKK3 was verified by double luciferase assay; RT-PCR was used to detect the expression of miR-1290 and DKK3 mRNA in SKOV3 cells; the activity of SKOV3 cells was detected by MTT assay; the apoptosis of SKOV3 cells was detected by flow cytometry; cell scratch test and Transwell test were used to detect the migration and invasion abilities of SKOV3 cells; Western blot was used to detect the expression of protein.Results:The double luciferase experiment showed that miR-1290 had a targeting relationship with DKK3, and miR-1290 could negatively target DKK3; Compared with miR-1290 NC group [24, 48, 72 h: (0.53 ± 0.05), (0.82 ± 0.06), (1.24 ± 0.06) ], MB group [24, 48, 72 h: (0.43 ± 0.06), (0.71 ± 0.03), (1.03 ± 0.03) ], miR-1290 inhibitor group [24, 48, 72 h: (0.41 ± 0.03), (0.66 ± 0.04), (0.78 ± 0.05) ], miR-1290 inhibitor MB group [24, 48, 72 h: (0.33 ± 0.04), (0.54 ± 0.05), (0.67 ± 0.06) ] SKOV3 cell proliferation activity decreased significantly ( P<0.05), compared with the migration rate and invasion number of SKOV3 cells in miR-1290 NC group [ (45.98 ± 4.11) %, (235.14 ± 5.78) ], the migration rate and invasion number of SKOV3 cells in MB group [ (36.77 ± 4.24) %, (189.57 ± 4.58) ], miR-1290 inhibitor group [ (32.14 ± 3.78) %, (165.35 ± 5.01) ], and miR-1290 inhibitor MB group [ (20.40 ± 3.01) %, (86.21 ± 4.23) ] decreased significantly,the expression of DKK3 mRNA and protein, apoptosis rate, apoptosis promoting protein C-Caspase-3 and Bax in SKOV3 cells increased greatly ( P<0.05) ; the above indexes of SKOV3 cells in miR-1209 NC group and Control group had no great difference ( P>0.05) . Conclusion:MiR-1290 can negatively regulate the expression of DKK3, while ultrasound microbubble can down regulate the expression of miR-1290 and up regulate the expression of DKK3, thereby inhibiting the proliferation, migration and invasion of ovarian cancer SKOV3 cells and promoting the apoptosis of cancer cells.

Effect of miR1290 on the growth of endometrial cancer and the related mechanism / 中华肿瘤杂志

Objective: To explore the expression of miR1290 in endometrial cancer tissues and its relationship with the pathological grade, and to find out the effect of miR1290 on biological characteristics of endometrial cancer cells and its mechanism. Methods: A total of 38 cases of endometrioid adenocarcinoma tissues, 10 cases of adjacent tissues and 23 cases of normal endometrial tissues were collected in Provincial Hospital Affiliated to Shandong University from May 2020 to October 2020. The expression of miR1290 was detected by reverse transcription polymerase chain reaction (RT-PCR). The expressions of miR1290 in endometrial cancer cells including KLE and Ishikawa were knocked down by lentiviral transfection. Cell counting kit 8 (CCK-8) test and colony formation test were used to detect cell proliferation ability, wound healing and Transwell test were used to detect cell invasion and migration ability, western blot was used to detect the expressions of epithelial-mesenchymal transition (EMT), phospholipids acylinositide 3-kinase (PI3K)/Akt and Wnt/β-catenin pathway related proteins. Results: The relative expressions of miR1290 in endometrial cancer tissues were 5.40±3.20, which was 1.55 times of normal endometrial tissues (P<0.01) and 1.75 times of adjacent tissues (P<0.01). The relative expressions of miR1290 in 17 cases of endometrial tissues at proliferative stage and 6 cases of endometrial tissues at secretory stage were 3.00±1.08 and 4.97±0.58, respectively, and the difference was statistically significant (P<0.01). In KLE cells and Ishikawa cells, the expression of miR1290 in miR1290 knockdown (Sh-miR1290) group was decreased when compared with the negative control (Sh-NC) group. The absorbance value of Sh-miR1290 group detected by the CCK-8 method and the colony formation rate detected by the colony formation experiment were both increased, the number of cells penetrating the basement membrane in the Transwell experiment and the wound healing rate in the scratch experiment were decreased (P<0.05). In KLE cells, knockdown of miR1290 reduced the expressions of EMT-related proteins including N-cadherin, Vimentin, Snail and Slug(P<0.05), and the expressions of PI3K and P-Akt/Akt (P<0.05), while there was no significant change in the expressions of Wnt and β-catenin (P>0.05). In Ishikawa cells, knockdown of miR1290 decreased the expressions of EMT-related proteins including N-cadherin, Snail and Slug, and the expressions of Wnt and β-catenin, increased the expression of E-cadherin (P<0.05), while there was no significant change in the expressions of PI3K and P-Akt/Akt (P>0.05). Conclusions: The expressions of miR1290 in endometrial cancer tissues are higher than that in the adjacent tissues and normal endometrial tissues. Knockdown of miR1290 expression can promote the proliferation of endometrial cancer cells, but inhibit cell invasion, migration and EMT ability through the PI3K/Akt and Wnt/β-catenin pathways.

Prognostic value of plasma miR-1290 expression in patients with oral squamous cell carcinoma / 华西口腔医学杂志

OBJECTIVE@#To investigate the prognostic value of plasma miR-1290 in patients with oral squamous cell carcinoma (OSCC).@*METHODS@#Seventy patients with OSCC admitted to Danzhou People's Hospital from January 2014 to December 2018 were included in this study. Real-time fluorescence quantitative polymerase chain reaction (PCR) was used to detect the expression of miR-1290 in these patients. The optimal cut-off value of plasma miR-1290 expression was determined by the ROC curve method, and patients with OSCC were divided into the high (n=31) and low (n=39) miR-1290-expressing groups. The clinicopathological features of the two groups were compared, and survival curves were drawn using the Kaplan-Meier method. Risk factors affecting the poor prognosis of patients were analyzed using univariate and multivariate COX regression models.@*RESULTS@#The expression level of plasma miR-1290 in the OSCC group was significantly lower than that in the control group (0.65±0.14 vs. 2.06±0.90; t=13.912, P<0.001). The low expression of plasma miR-1290 appeared to be related to the clinical stage, differentiation degree, tumor diameter, and lymph node metastasis of OSCC (P<0.05). Survival analysis showed that the overall survival rate and the progression-free survival rate of the low-miR-1290 group were significantly lower than that of the high-miR-1290 group (P<0.01). Multivariate COX regression analysis showed that clinical stage, lymph node metastasis, and plasma miR-1290<1.14 were independent risk factors for the poor prognosis of patients with OSCC (P<0.05).@*CONCLUSIONS@#The expression level of plasma miR-1290 in patients with OSCC significantly decreased, and the low expression of miR-1290 is related to the short survival time of OSCC patients. Thus, miR-1290 may be a potential marker predicting the poor prognosis of OSCC.