Résumé
Eleven monoterpene glucosides were isolated from a water decoction of Monochasma savatieri by column chromatography over macroporous adsorbent resin, MCI resin, Sephadex LH-20, and HW-40C, combined with preparative TLC, reversed phase HPLC, and flash column chromatographic techniques. Their structures were elucidated by comprehensive analysis of spectroscopic data, along with acidic and enzymatic hydrolysis as well as electronic circular dichroism (ECD) and NMR calculations, including six new compounds (1-4, 7 and 8), named monochasides A-D, G and H, respectively. Comparing the reported data of 9-hydroxylinaloyl 3-O-β-D-glucoside (5), (6Z)-9-hydroxylinaloyl 3-O-β-D-glucoside (6), and kankanoside D1 (9) with those obtained in this study, the absolute configurations of 6 and 9 were proved for the first time. Other two compounds were identified as 8-hydroxygeraniol 1-O-β-D-glucopyranoside (10) and 8-hydroxygeraniol 8-O-β-D-glucopyranoside (11), respectively.
Résumé
In order to identify the endophytic fungus MD76 and isolate its secondary metabolite. METHODS: The identification of strain MD76 is based on morphological characteristics and sequence alignment analysis of 18s rDNA ITS, the secondary metabolites were isolated by chromatography technology; and their structures were elucidated by extensive spectroscopic analysis. RESULTS: The strain MD76 was identified as Phyllosticta capitalensis; and seven compounds were gained, including benzoic acid(1), 4-hydroxybenzoic acid(2), 4-hydroxybenzene propanoic acid(3), paeoniflorin(4), oxypaeoniflorin(5), gallic acid(6), apigenin(7). CONCLUSION: The endophytic fungus P. capitalensis was gained firstly from Paeonia suffruticosa and seven compounds were isolated from P. capitalensis for the first time.
Résumé
OBJECTIVE: To study the chemical constituents of the seed cakes of Paeonia ostii. METHODS: Chemical constituents were isolated by Toyopearl HW-40, Sephadex LH-20, preparative HPLC, and silica gel column chromatographies. The structures were elucidated on the basis of spectral data analysis. RESULTS: Seven monoterpene glucosides, paeoniflorin (I), oxypaeoniflorin (II), abiflofin(III), 4″- hydroxylalbiflorin(IV), 1-O-β-D-glucopyransoylpaeonisuffron(V), β-gentiobi-osylpaeoniflorin(VI), and abiflofin R1 (VII) were isolated from the seed cake of Paeonia ostii. CONCLUSION: All the compounds are isolated from the seed cakes of Paeonia ostii for the first time.