Application of Giemsa stain for easy detection of Trichinella spiralis muscle larvae

The application of Giemsa technique to stain compressed diaphragm samples obtained from rodents experimentally infected with Trichinella spiralis is described. Diaphragm samples from rats heavily infected with 20 muscle larvae per gram of body weight (20 ML/gbw) were cut into several pieces and stained with Giemsa; on the other hand, whole diaphragms from slightly infected mice (1 ML/gbw) were also stained with Giemsa. Besides, muscle samples were also stained with Giemsa. Observation at 10 x magnification revealed that both ML and nurse cells (NC) look as bluish structures clearly contrasting with the pinkish color of the non-infected muscle fibers. NC in the diaphragms of mice could be easily observed at naked eye as blue points contrasting with the pink surrounding areas formed by the non-infected muscle fibers. Among NC observed in the diaphragms of rats infected with 20 ML/gbw, 4.4% was multiple infection. These findings were confirmed in sectioned and hematoxylin-eosin stained specimens. This data could be usefulness for a rapid diagnosis of trichinellosis in post-mortem mammals without magnification procedures.

Application of Giemsa stain for easy detection of Trichinella spiralis muscle larvae

The application of Giemsa technique to stain compressed diaphragm samples obtained from rodents experimentally infected with Trichinella spiralis is described. Diaphragm samples from rats heavily infected with 20 muscle larvae per gram of body weight (20 ML/gbw) were cut into several pieces and stained with Giemsa; on the other hand, whole diaphragms from slightly infected mice (1 ML/gbw) were also stained with Giemsa. Besides, muscle samples were also stained with Giemsa. Observation at 10 x magnification revealed that both ML and nurse cells (NC) look as bluish structures clearly contrasting with the pinkish color of the non-infected muscle fibers. NC in the diaphragms of mice could be easily observed at naked eye as blue points contrasting with the pink surrounding areas formed by the non-infected muscle fibers. Among NC observed in the diaphragms of rats infected with 20 ML/gbw, 4.4% was multiple infection. These findings were confirmed in sectioned and hematoxylin-eosin stained specimens. This data could be usefulness for a rapid diagnosis of trichinellosis in post-mortem mammals without magnification procedures.

Motphological characteristics and Localization of Tree Shrew's Thymic Nurse Cells under Electron Microscope / 昆明医科大学学报

This paper reports the observations on morphological characteristics and locations of TNC in tree shrew(Tupaia beiangeri Chinensis)under EM.It is confirmed that all the TNC in 8 newborn tree shrews locate in the cortical area of the thymus.The characteristics of its ultrastructure are similar to what have been observed in the thymic cortex of human/mouse. The desmosomes of TNC and the structure that is in contact with the capillary in the human TNC have not been observed in the tree shrew.

THE IMMUNOCYTOCHEMICAL OBSERVATION OF NEUROENDOCRINE CHARACTERISTIC IN MICE THYMIC NURSE CELLS / 解剖学报

The arginine vasopressin (AVP) and oxytocin (OT) in thymic nurse cells (TNCs) of mice were stained by the method of immunogold and indfrect immunofluorescence, PAP immunocytochemical technique. TNCs of mice were shown immunocytochemical reactivity with antfserum of AVP and OT. The posttive areas were much denser near the cells membrane and around TNC-lymphocytes (TNC-Ls). It was implicated that TNCs might serve as neuroendocrine cells and might play a role in TNC-Ls differentiation via secreting AVP and OT.

EFFECT OF ORCHIDECTOMY ON MURINE THYMIC NURSE CELLS (TNC) / 中国免疫学杂志

The results obtained were as follows:It was found that the orchidectomi-zed animals had a significantlyhigher TNC count per thymus.Inaddition,thymus weight was increa-sed and tymocytes,peripheral WBCand lymphocytes counts were elevatedin orchidectomized mice,comparedwith sham-operafed animals.

HISTOLOGICAL AND HISTOCHEMICAL CHANGES OF THYMIC NURSE CELLS AND IMMUNE ORGANS OF MOUSE AFTER THE INOCULATION OF LEUKEMIC CELLS / 解剖学报

It was discovered in this experiment that 3 days after inoculation of the ascitic fluid from P_(388) leukemic mice, the positive percentages of AcP and ?-Gase of the lymphocytes within the TNC of the recipients were increased significantly, while the positive percentage of ANAE was decreased. All the positive percentages of the three enzymes of the thymocytes within the thymus were increased significantly, but no abnormal structural changes were seen in the thymus. No histological and cytochemical changes were found in the lymph nodes and the spleen. Nine days after inoculation, the leukemia cell infiltration, hemorrhage and cell degeneration occurred in all the thymus, lymph nodes and spleen of the mouse. The normal structures of the organs were destroyed. The positive percentages of the cytochemical reactions of the three enzymes in the lymphocytes were all increased significantly. At this stage, TNC was very difficult to be isolated from the thymus. The results indicate that thymus and TNC may have a close relationship with the development of this type of leukemia.

HISTOCHEMICAL AND ELECTRON MICROSCOPICAL OBSERVATION ON THYMIC NURSE CELL OF HUMAN FETUSES / 解剖学报

This paper reports some histochemical,scanning and transmission electron mic-roscopical features of thymic nurse cell(TNC)of human fetuses.The results showedthat TNCs were all negative for ANAE,AcP,AlP,ATPase,5'-Nase and PAS.TNCcontained various numbers of lymphocytes.Some intracytoplasmic lymphocytes werepositive for ANAE,AcP,AlP and ATPase.Their ANAE positive percentage deter-mined were 26.8%.However,ANAE positive percentage of extracytoplasmic lym-phocytes of TNC were 51.3%.Our data demonstrated that there was significant dif-ference between these two ANAE positive percentages,P