Effect of Rhizobium, PSB and Mo on the Growth of Different Varieties of Kabuli Chickpea (Cicer kabulinum L.)

The present investigation was conducted with the objective of finding out the Effect of Rhizobium, PSB and Mo on the Growth of Different Varieties of Kabuli Chickpea. The study comprised six treatments of Inoculants I1-Control, I2-Rhizobium(Rh.)+phosphate solubilizing bacteria (PSB) seed inoculation, I3-Molybdenum (Mo)@0.5 g AM* kg-1 seed, I4-Molybdenum @1.0 g AM kg-1 seed, I5-Rh.+PSB+Mo seed treatment @0.5g AM kg-1 seed, I6-Rh.+PSB+Mo seed treatment 1.0g AM kg-1 seed and two varieties V1-RVSJKG 102, V2-Phule G 0517, evaluated in Factorial randomized block design (FRBD) with three replications. The results of the present study revealed that the seed inoculation, I6Rh.+ PSB + Mo@1 g AM kg-1 seed was found to be the best among other inoculants with respect to growth in chickpeas, and Variety Phule G 0517 produced higher values of growth and yield attributing.

Enhancing Nutrient Content and Uptake in Chickpea through Phosphorus and PSB Inoculation in Custard Apple Based Agri-horti System

The field experiment was conducted during the Rabi season of 2018-19 at the Agricultural Research Farm of Rajiv Gandhi South Campus, Banaras Hindu University, Barkachha, Mirzapur, Uttar Pradesh, India. This study aimed to investigate the effects of varying levels of phosphorus and inoculation with Phosphate Solubilizing Bacteria (PSB) on nutrient content and uptake in chickpea (Cicer arietinum L.) in a custard apple-based agri-horticultural system. The experimental design employed a Factorial Randomized Block Design with three replications and twelve treatments, resulting in a total of 36 plots. The treatments ranged from different levels of phosphorus and PSB inoculation, denoted as T1 to T12. The study assessed the impact of these treatments on nitrogen (N), phosphorus (P) and potassium (K) content in grain and straw, as well as their uptake by chickpea. Findings reveal that higher PSB levels and phosphorus applications led to increased nitrogen and phosphorus content and uptake in both grain and straw, demonstrating a significant synergistic effect. Conversely, potassium content and uptake exhibited limited response to PSB inoculation. Moreover, protein content and yield in grain and straw significantly improved with these treatments.

Effects of Biofertilizers and Phosphorus on Growth and Yield of Mustard

The field experiment titled “Effect of Biofertilizers and Phosphorous on Growth and Yield of Mustard” was conducted during Rabi 2022 at Crop Research Farm, Department of Agronomy, SHUATS, Prayagraj (U.P). The experiment was laid out in randomized block design with nine treatments and control which are replicated thrice. The treatments viz T1: PSB + Phosphorus 40 kg/ha, T2: PSB + Phosphorus 50 kg/ha, T3: PSB + Phosphorus 60 kg/ha, T4: VAM + Phosphorus 40 kg/ha, T5: VAM + Phosphorus 50 kg/ha, T6: VAM + Phosphorus 60 kg/ha, T7: PSB + VAM + Phosphorus 40 kg/ha, T8: PSB + VAM + Phosphorus 50 kg/ha, T9: PSB + VAM + Phosphorus 60 kg/ha. The results obtained that growth parameters viz. plant height (180.40 cm), Number of branches (18.10), dry weight (42.01 g/plant), Significantly higher yield parameters viz. number of silique/plant (314.47), test weight (6.80 g) and number of seeds/silique (16.50). were recorded higher with the application of PSB + VAM + Phosphorus 60 kg/ha.

Effect of Vermicompost, Biofertilizers and in Organic Fertilizers on Nutrient Uptake and Physico-chemical Properties of Soil: A Case Study of Chickpea (Cicer arietinum L.)

A field trial was conducted during Rabi 2020-21 in randomized block design with three replications and seven treatments comprising individual and combined application of various organic and inorganic nutrient sources at Research Farm, MMU Sadopur (Ambala), Haryana. The experiment was intended to evaluate the effect of vermicompost, biofertilizers and inorganic fertilizers applied in chickpea on nutrient uptake and physico-chemical properties of soil pH (8.24). The highest organic carbon (0.58%), electrical conductivity (0.51 dS m-1), particle density (2.50 g/cm3) and porosity (48.47%) along with maximum available N (126.5 kg ha-1), P (23.2 kg/ha) and K (98.33 kg/ha) were observed in the treatment of T6 i.e., 3 t/ha Vermicompost + Rhizobium + PSB. Similarly, the nutrient content (N, P and K) was maximum in seed and stover of chickpea harvested from the plots receiving 3 t/ha Vermicompost + Rhizobium + PSB, which was statistically at par with T7 i.e., 2.5 t/ha Vermicompost + Rhizobium + PSB. The highest net return was recorded in plots treated with 50% RDF (Recommended dose of fertilizer) + Rhizobium + PSB and 75% RDF + Rhizobium + PSB with a benefit cost ratio (B: C) of 3.2 and 3.1, respectively. However, the seed yield in integrated nutrient management was comparable to recommended dose of chemical fertilizers

Role of Biofertilizers in Chickpea: A Review

Compared to when both rhizobium and the bacteria that solubilize phosphate are inoculated separately, the interaction effect between the two inoculations gradually increases the growth parameters and yield production. Rhizobium and phosphate solubilizing bacteria (PSB) had shown advantage in enhancing chickpea productivity, cost effective, ecofriendly and renewable sources of plant nutrients. Biofertilizers have the ability to improve the growth and nodulation of chickpea enables it to withstand the periods of drought. Utilising Rhizobium's symbiotic properties to increase the nitrogen supply to agricultural plants.

Influence of Integrated Nutrient Management on the Productivity of kharif Redgram (Cajanus cajan L.)

A field experiment was conducted during kharif season of 2021-22 at Agricultural Research Station, Tandur, Vikarabad, Professor Jayashankar Telangana State Agricultural University (PJTSAU) under deep black soils to study the effect of integrated nutrient management on the growth and yield of kharif redgram (Cajanus cajan L.). The experiment was laid out in Randomized block design with 9 treatments in 3 replications. Treatments comprised of T1: Control, T2: 50% RDF (10:25:0 NPK kg ha-1) + 50% FYM (2.5 t ha-1), T3: 75% RDF (15:37.5:0 NPK kg ha-1) + 25% FYM (1.25 t ha-1), T4: 100% RDF (20:50:0 NPK kg ha-1), T5: 50% RDF + FYM @ 5 t ha-1 + Rhizobium (Seed treatment) + PSB (Seed treatment), T6: 75% RDF + FYM @ 5 t ha-1 + Rhizobium (Seed treatment) + PSB (Seed treatment), T7: 100% RDF + FYM @ 5 t ha-1 + Rhizobium (Seed treatment) + PSB (Seed treatment), T8: 100% RDF + Rhizobium (Seed treatment) + PSB (Seed treatment) and T9: FYM @ 5 t ha-1 + Rhizobium (Seed treatment) + PSB (Seed treatment). Among the treatments, application of 100% RDF, FYM @ 5 t ha-1, Rhizobium and PSB recorded significantly higher seed yield (1898 kg ha-1) and stalk yield (6275 kg ha-1).

Toxicity of Herbicide, Paraquat Dichloride and Insecticide, Lambda-cyhalothrin on Phosphate Solubilizing Bacteria, Pantoa dispersa in Aquatic Ecosystems

Aim: The study was aimed to determine the toxicity of paraquat dichloride and lambda-cyhalothrin on phosphate solubilizing bacteria, Pantoa dispersa in aquatic ecosystems.Study Design: This study employs randomized block design, statistical analysis of the data and interpretation.Place and Duration of the Study: Soil sample was collected from the root nodules of leguminous plants in a sterile polythene bag from the Elele, in Etche L.G.A, Rivers State. The fresh water sample was collected from Bane town in Khana L.G.A, brackish water sample was collected from Choba river in Obio/Akpor L.G.A while the marine water was collected from Bonny River of Bonny L.G.A., all of Rivers State, Nigeria. The samples were collected aseptically and transported in an ice-pack immediately to the Rivers State University, Microbiology laboratory for analysis. The study lasted for three months.Methodology: The bacterium, Pantoa dispersa was isolated and identified based on conventional and molecular characterization from water and soil samples. Different concentrations (3.13%, 6.25, 12.50, 25.00%, 50.00% and 75.00%) and the control (0%) of the herbicide (paraquat dichloride) and insecticide, Lambda-cyhalothrin were prepared using fresh, brackish and marine water samples and 10ml of the test organism, Pantoa dispersa was introduced and the survival count was determined at 0, 4hr, 8hr, 12hr and 24hr. The Mean Lethal Concentration (LC50) of the insecticide and herbicide on Pantoa dispersa in the three aquatic ecosystem was determined.Results: The LC50 of the herbicide (Paraquat dichloride) was recorded as 15.8% in brackish water, 17.37% in fresh water and 27.44% in marine water. While the LC50 of the insecticide, Lambda-cyhalothrin to Pantoa dispersa was 26.84% in fresh water, 27.26% in brackish water and 32.33% in marine water.Conclusion: From the study, the herbicide, Paraquat dichloride was more toxic in the three aquatic ecosystems compared to the insecticide, Lambda-cyhalothrin. The use of these agrochemicals should be monitored as they result in the mortality of beneficial soil bacteria like Pantoa dispersa which is phosphate solubilizing bacteria in aquatic ecosystems.

Optimization of soybean yield in ultisols through adaptive varieties screening and plant growth promoting Rhizobacter

This research was aimed at obtaining varieties of soybean adaptive to acid soils and to obtain Plant Growth-Promoting Rhizobacteria (PGPR) isolates that can improve the agronomic characteristics of soybean and increase the ultisols fertility. This research was conducted in two-stages research on Sampali Village, Percut Sei Tuan sub-District, Deli Serdang District, North Sumatra Province, Indonesia from August 2019 until March 2020. The first stage (adaptive varieties screening) using the non-factorial Randomized Block Design (RBD) with the varieties of Argomulyo, Wilis, Kaba, Dena-1, Devon-1, Dega-1, Demas-1, Burangrang, Detam-1, and Kipas Merah. The second stage (application of PGPR isolates singly and in combinations) using the factorial RBD, the first factor of applicative single and the combination of PGPR isolates, the second factor of adaptive varieties including Detam-1 and Wilis. Data were analyzed with ANOVA and followed by DMRT at P<0.05. The results showed that the Detam-1 and Wilis varieties had significantly higher yield per plant of 14.73 g and 14.54 g, respectively, than other varieties. The applications of a single and combination of PGPR isolates significantly increased the number of branches, stem diameter, plant height, yield per plant, soil pH, organic-C, available-P, and total-N and decreased the soil C/N. The Detam-1 variety had the higher in yield per plant compared to Wilis variety. The isolates combination of Rhizobium leguminosarum+Rhizobium sp2+Bacillus sp+Burkholderia sp for Detam-1 and Wilis varieties can be recommended to support the growth and yield of soybean on ultisols.

Agronomic efficiency and profitability of cotton on integrated use of phosphorus and plant microbes / Eficiência e rentabilidade agronômica do algodão no uso integrado de fósforo e micróbios vegetais

Cotton crop, plays a significant role in Pakistan's economy by ruling a prominent place in edible oil and local textile industry. Phosphorus (P) inaccessibility and deficiency of soil organic matter are the key restraints for low crop productivity in cotton. Therefore, a two years field study was designed during 2014-15, to explore the influence of phosphate solubilizing bacteria (PSB), farmyard manure (FYM), poultry manure (PM) and inanimate sources of P on various physiological, growth, yield and quality parameters of cotton crop at CCRI Multan. Field responses of seeds inoculated with two distinctive phosphate solubilizing bacteria (PSB) strains viz. S0 = control, S1 =strain-1, S2 = strain-2 and eight organic, inorganic P sources viz., P0= control, P1 = 80 kg ha-1 P from inorganic source, P2 = 80 kg ha-1 P from FYM, P3 = 80 kg ha-1 P from PM, P4 = 40 kg ha-1 P from FYM + 40 kg ha-1 P from inorganic source, P5 = 40 kg ha-1 P from PM + 40 kg ha-1 P from inorganic source, P6 = 80 kg ha-1 P from FYM + 40 kg ha-1 P from inorganic source, P7 = 80 kg ha-1 P from PM + 40 kg ha-1 P from inorganic source and P8 = 40 kg ha-1 P from FYM + 40 kg ha-1 P from PM were evaluated. Results revealed that inoculation of seeds with PSB and collective use of inorganic and organic sources of P had considerably increased the yield contributing attributes in cotton. However, the treatment P7 (80 kg P ha-1 from PM + 40 kg P ha-1 from inorganic source) in coincidence with seeds inoculated with PSB (S1) produced taller plant, maximum boll weight, significantly higher LAI and CGR. Significantly higher seed cotton yield, lint yield, fiber length and maximum BCR of 1.95 and 1.81 was also obtained from the P7 treatment during both crop-growing seasons. In conclusion, combined use of 80 kg P ha-1 from PM + 40 kg P ha-1 from inorganic source and cotton seeds inoculated with strain-1 improved phosphorus uptake ensuing in greater consumption of photo-assimilates for maximum growth and yield.

A safra de algodão, desempenha um papel significativo na economia do Paquistão, ao ocupar um lugar de destaque no óleo comestível e na indústria têxtil local. A inacessibilidade e a deficiência de fósforo (P) da matéria orgânica do solo são as principais restrições para a baixa produtividade das culturas em algodão. Portanto, um estudo de campo de dois anos foi desenvolvido durante 2014-15, para explorar a influência de bactérias solubilizantes de fosfato (PSB), esterco de capoeira (FYM), esterco de aves (PM) e fontes inanimadas de P sobre vários fatores fisiológicos, crescimento, rendimento e parâmetros de qualidade da cultura do algodão no CCRI Multan. Respostas de campo de sementes inoculadas com duas cepas distintas de bactérias solubilizantes de fosfato (PSB) viz. S0 = controle, S1 = cepa-1, S2 = cepa-2 e oito fontes orgânicas de P inorgânicas viz., P0 = controle, P1 = 80 kg ha-1 P de fonte inorgânica, P2 = 80 kg ha-1 P de FYM, P3 = 80 kg ha-1 P de PM, P4 = 40 kg ha-1 P de FYM + 40 kg ha-1 P de fonte inorgânica, P5 = 40 kg ha-1 P de PM + 40 kg ha-1 P de fonte inorgânica, P6 = 80 kg ha-1 P de FYM + 40 kg ha-1 P de fonte inorgânica, P7 = 80 kg ha-1 P de PM + 40 kg ha-1 P de fonte inorgânica e P8 = 40 Foram avaliados kg ha-1 P da FYM + 40 kg ha-1 P da PM. Os resultados revelaram que a inoculação de sementes com PSB e o uso coletivo de fontes inorgânicas e orgânicas de P aumentaram consideravelmente os atributos que contribuem para a produtividade no algodão. No entanto, o tratamento P7 (80 kg P ha-1 da PM + 40 kg P ha-1 da fonte inorgânica) em coincidência com sementes inoculadas com PSB (S1) produziu planta mais alta, peso máximo de cápsula, IAF e CGR significativamente maiores. Produtividade significativamente maior do algodão, rendimento de fiapos, comprimento da fibra e BCR máximo de 1,95 e 1,81 também foi obtida a partir do tratamento P7 durante as duas épocas de cultivo. Em conclusão, o uso combinado de 80 kg P ha-1 de PM + 40 kg P ha-1 de fonte inorgânica e sementes de algodão inoculadas com a cepa-1 melhoraram a captação de fósforo, resultando em maior consumo de foto assimilados para obter crescimento e produtividade máximos.

Isolation and identification of phosphate solubilizing bacteria from indigenous microorganisms (IMO) of cow rumen in East Java, Indonesia as eco-friendly biofertilizer

Aims@#Phosphate is an essential nutrient required for plant growth, but its solubility in the soil is relatively low (0.1%). Microbes can dissolve phosphate to meet crop requirements. This study aimed to isolate phosphate solubilizing bacteria from indigenous microorganisms (IMO) of cow rumen.@*Methodology and results@#The selection of isolates on a Pikovskaya medium was using a clear zone index and a spectrophotometer for phosphate solubilization measurements. Hypersensitivity was tested on tobacco leaves and tested antagonists within isolates. The results found that four selected isolates had the highest phosphate dissolving potential, namely, MTA1, SMAD1, SMAD2, and SMAD3. The culture of selected isolates on plate media showed that the morphological characters of the four colonies are the same. They had round form (circular), the edge of the colony were smooth, flat elevation, white and cream color. Isolate MTA1 had the highest phosphate solubilizing activity compared to the others. The isolate that showed the highest phosphate solubilizing activity were identified based on 16S rRNA gene. The result of molecular identification showed that strain MTA1 was closely related to Lactobacillus plantarum with a similarity level of 99%. L. plantarum performed the highest ability to form a clear zone (7.66 mm). The highest concentration of soluble phosphate was observed on day 5 (278.42 mg/L).@*Conclusion, significance, and impact of the study@#Lactobacillus plantarum which was isolated from the IMO of cow rumen in East Java, Indonesia was identified as one of the phosphate solubilizing bacteria that are useful for the development of eco-friendly biofertilizer. The application of phosphate solubilizing microbes can be used to increase the soil fertility.

Impact of phosphate solubilizing bacteria on wheat (Triticum aestivum ) in the presence of pesticides / Impacto das bactérias solubilizantes de fosfato no trigo (Triticum aestivum ) na presença de pesticidas

Abstract Three phosphate solubilizing bacteria were isolated and identified by 16S rRNA sequencing as Pseudomonas putida, Pseudomonas sp and Pseudomonas fulva . The strains were subjected to plant biochemical testing and all the PGPR attributes were checked in the presence of pesticides (chlorpyrifos and pyriproxyfen). The phosphate solubilizing index of strain Ros2 was highest in NBRIP medium i.e 2.23 mm. All the strains showed acidic pH (ranges from 2.5-5) on both medium i.e PVK and NBRIP. Strain Ros2 was highly positive for ammonia production as well as siderophore production while strain Rad2 was positive for HCN production. The results obtained by the strains Rad1, Rad2 and Ros2 for auxin production were 33.1, 30.67 and 15.38 µg ml-1, respectively. Strain Rad1 showed 16% increase in percentage germination in comparison to control in the presence of pesticide stress. Most promising results for chlorophyll content estimation were obtained in the presence of carotenoids upto 6 mgg-1 without stress by both strains Rad1 and Rad2. Study suggests that especially strain Ros2 can enhance plant growth parameters in the pesticide stress.

Resumo Três bactérias solubilizantes de fosfato foram isoladas e identificadas por seqüenciamento de rRNA 16S como Pseudomonas putida, Pseudomonas sp e Pseudomonas fulva. As estirpes foram submetidas a testes bioquímicos de plantas e todos os atributos PGPR foram verificados na presença de pesticidas (clorpirifos e piriproxifeno). O índice de solubilização de fosfato da estirpe Ros2 foi mais elevado no meio NBRIP, isto é, 2,23 mm. Todas as estirpes apresentaram um pH ácido (varia de 2,5-5) em ambos os meios, isto é PVK e NBRIP. A estirpe Ros2 foi altamente positiva para a produção de amoníaco, bem como a produção de sideróforos enquanto a estirpe Rad2 foi positiva para a produção de HCN. Os resultados obtidos pelas estirpes Rad1, Rad2 e Ros2 para a produção de auxina foram 33,1, 30,67 e 15,38 μg ml-1 , respectivamente. A deformação Rad1 mostrou aumento de 16% na germinação percentual em comparação com o controlo na presença de stress de pesticida. Os resultados mais promissores para a estimativa do teor de clorofila foram obtidos na presença de carotenóides até 6 mgg-1 sem estresse por ambas as cepas Rad1 e Rad2. Estudo sugere que especialmente a estirpe Ros2 pode melhorar parâmetros de crescimento de plantas no estresse de pesticidas.

Characterization of phosphate solubilizing bacteria and nitrogen fixing bacteria from limestone mining region

Aims: Phosphate and nitrogen are major macronutrients needed by plants. Phosphates in the soil are present in the organic and inorganic form. The amounts of phosphate and nitrogen in marginal soil can be increased by plant growth promoting rhizobacteria (PGPR). The aim of this study was to isolate and characterize phosphate solubilizing bacteria which has ability to fix nitrogen from the soil around limestone mining area. Methodology and results: There were 22 isolates that could solubilize phosphate and fix nitrogen. There were 9 isolates that could not cause hypersensitivity symptoms (necrotic) on tobacco leaf. Quantitative assay of phosphate solubilizing was done by colorimetric method. Quantitative assay of phosphate showed that isolate GPC1.7 had the highest phosphate solubilizing activity on Pikovskaya broth (450 mg/L) on the 6th and 7th day of incubation whereas isolate GPA2.2 had the highest nitrogen fixing activity (0.162 ppm/h), measured with Acetylene Reduction Assay whereas nitrogenase activity of GPC1.7 was unidentified. Isolate GPA2.1 and GPA2.2 were Gram negative bacteria whereas isolate GPC1.7 was Gram positive bacteria. Identification based on 16S rRNA gene showed that GPA2.1 was closely related to Pseudomonas psychrotolerans, GPA2.2 was closely related to Stenotrophomonas maltophilia, GPC1.7 was closely related to Bacillus megaterium and B. aryabhattai. Conclusion, significance and impact of study: Phosphate solubilizing bacteria and nitrogen fixing bacteria isolate could be further used for revegetation process of the ex-limestone mining area.

Agronomic efficiency and profitability of cotton on integrated use of phosphorus and plant microbes

Abstract Cotton crop, plays a significant role in Pakistans economy by ruling a prominent place in edible oil and local textile industry. Phosphorus (P) inaccessibility and deficiency of soil organic matter are the key restraints for low crop productivity in cotton. Therefore, a two years field study was designed during 2014-15, to explore the influence of phosphate solubilizing bacteria (PSB), farmyard manure (FYM), poultry manure (PM) and inanimate sources of P on various physiological, growth, yield and quality parameters of cotton crop at CCRI Multan. Field responses of seeds inoculated with two distinctive phosphate solubilizing bacteria (PSB) strains viz. S0 = control, S1 =strain-1, S2 = strain-2 and eight organic, inorganic P sources viz., P0= control, P1 = 80 kg ha-1 P from inorganic source, P2 = 80 kg ha-1 P from FYM, P3 = 80 kg ha-1 P from PM, P4 = 40 kg ha-1 P from FYM + 40 kg ha-1 P from inorganic source, P5 = 40 kg ha-1 P from PM + 40 kg ha-1 P from inorganic source, P6 = 80 kg ha-1 P from FYM + 40 kg ha-1 P from inorganic source, P7 = 80 kg ha-1 P from PM + 40 kg ha-1 P from inorganic source and P8 = 40 kg ha-1 P from FYM + 40 kg ha-1 P from PM were evaluated. Results revealed that inoculation of seeds with PSB and collective use of inorganic and organic sources of P had considerably increased the yield contributing attributes in cotton. However, the treatment P7 (80 kg P ha-1 from PM + 40 kg P ha-1 from inorganic source) in coincidence with seeds inoculated with PSB (S1) produced taller plant, maximum boll weight, significantly higher LAI and CGR. Significantly higher seed cotton yield, lint yield, fiber length and maximum BCR of 1.95 and 1.81 was also obtained from the P7 treatment during both crop-growing seasons. In conclusion, combined use of 80 kg P ha-1 from PM + 40 kg P ha-1 from inorganic source and cotton seeds inoculated with strain-1 improved phosphorus uptake ensuing in greater consumption of photo-assimilates for maximum growth and yield.

Resumo A safra de algodão, desempenha um papel significativo na economia do Paquistão, ao ocupar um lugar de destaque no óleo comestível e na indústria têxtil local. A inacessibilidade e a deficiência de fósforo (P) da matéria orgânica do solo são as principais restrições para a baixa produtividade das culturas em algodão. Portanto, um estudo de campo de dois anos foi desenvolvido durante 2014-15, para explorar a influência de bactérias solubilizantes de fosfato (PSB), esterco de capoeira (FYM), esterco de aves (PM) e fontes inanimadas de P sobre vários fatores fisiológicos, crescimento, rendimento e parâmetros de qualidade da cultura do algodão no CCRI Multan. Respostas de campo de sementes inoculadas com duas cepas distintas de bactérias solubilizantes de fosfato (PSB) viz. S0 = controle, S1 = cepa-1, S2 = cepa-2 e oito fontes orgânicas de P inorgânicas viz., P0 = controle, P1 = 80 kg ha-1 P de fonte inorgânica, P2 = 80 kg ha-1 P de FYM, P3 = 80 kg ha-1 P de PM, P4 = 40 kg ha-1 P de FYM + 40 kg ha-1 P de fonte inorgânica, P5 = 40 kg ha-1 P de PM + 40 kg ha-1 P de fonte inorgânica, P6 = 80 kg ha-1 P de FYM + 40 kg ha-1 P de fonte inorgânica, P7 = 80 kg ha-1 P de PM + 40 kg ha-1 P de fonte inorgânica e P8 = 40 Foram avaliados kg ha-1 P da FYM + 40 kg ha-1 P da PM. Os resultados revelaram que a inoculação de sementes com PSB e o uso coletivo de fontes inorgânicas e orgânicas de P aumentaram consideravelmente os atributos que contribuem para a produtividade no algodão. No entanto, o tratamento P7 (80 kg P ha-1 da PM + 40 kg P ha-1 da fonte inorgânica) em coincidência com sementes inoculadas com PSB (S1) produziu planta mais alta, peso máximo de cápsula, IAF e CGR significativamente maiores. Produtividade significativamente maior do algodão, rendimento de fiapos, comprimento da fibra e BCR máximo de 1,95 e 1,81 também foi obtida a partir do tratamento P7 durante as duas épocas de cultivo. Em conclusão, o uso combinado de 80 kg P ha-1 de PM + 40 kg P ha-1 de fonte inorgânica e sementes de algodão inoculadas com a cepa-1 melhoraram a captação de fósforo, resultando em maior consumo de foto assimilados para obter crescimento e produtividade máximos.

Assessment of Rice Associated Bacterial Ability to Enhance Rice Seed Germination and Rice Growth Promotion

ABSTRACT The application of beneficial bacteria has recently been used for sustainable agriculture. In current research, 71 bacterial isolates were obtained from rice plant and the rhizosphere soil of different paddy fields in Guilan province, Iran. After primitive investigation, 40 bacteria with typical predominant characteristics were selected. By PCR-RFLP of their 16S r-DNA gene, 8 Operational Taxonomic Units (OTUs) totally consisted of 33 isolates were obtained. From all of them, 8 isolates were selected for rice seed germination experiment, then, effective isolates were used for pot experiment to evaluate their ability for promoting rice growth. All of them were able to increase rice growth and yield, but in different potential. These tested isolates were identified as Alcaligenes faecalis (DEp8, O1R4), Pantoea ananatis (AEn1), Bacillus vietnamensis (MR5), Bacillus idriensis (MR2) and Stenotrophomonas maltophilia by partial sequencing of their 16S r-DNA gene. Among them, AEn1 and MR5 produced indole-3- acetic acid (IAA) in larger amounts than the other isolates and the isolates AEn1 and O1R4 were able to solubilize phosphate in higher amounts. According to the results obtained, it can be concluded that AEn1, O1R4 and MR5 can be considered as bacterial inoculants to use as alternatives for chemical fertilizers.

Caracterización de rizobacterias promotoras de crecimiento en plántulas de Eucalyptus nitens / Characterization of growth-promoting rhizobacteria in Eucalyptus nitens seedlings

Se aislaron bacterias rizosféricas y endófitas a partir de rizósfera y tejidos de raíz de árboles de Eucalyptus nitens con el objetivo de evaluar su capacidad de promover el crecimiento en plántulas de la misma especie en condiciones de invernadero. Los aislamientos que incrementaron el crecimiento de las plántulas fueron identificados y caracterizados por su capacidad de producir ácido indolacético (AIA), solubilizar fosfato y expresar la 1-aminociclopropano-1-carboxilato (ACC) desaminasa. Los 105 aislamientos obtenidos fueron morfológicamente diferentes y solo 15 promovieron significativamente el crecimiento de plántulas de E. nitens. Los máximos incrementos observados fueron en el peso seco aéreo (142 %) y de la raíz (135 %); también aumentaron la altura de las plantas (50 %) y el largo de raíces (45 %) de las mismas. Las rizobacterias pertenecieron a los géneros Arthrobacter, Lysinibacillus, Rahnella y Bacillus. Los aislados identificados como A. phenanthrenivorans 21 y B. cereus 113 incrementaron la emergencia de E. nitens a los 12 días en un valor promedio de 3,15 veces con relación al control. R. aquatilis aislado 78 presentó la mayor producción de AIA (97,5 ± 2,87 μg/ml) en presencia de triptófano y el mayor índice de solubilización de fósforo (2,4). B. amyloliquefaciens aislado 60 fue positivo para la actividad ACC desaminasa. Los resultados obtenidos indican el potencial de las rizobacterias estudiadas como promotoras de emergencia y crecimiento de plántulas de E. nitens y su posible uso como inoculantes, ya que presentan más de un mecanismo de acción asociado a la promoción del crecimiento.

Rhizospheric and endophytic bacteria were isolated from the rizosphere and root tissue of Eucalyptus nitens. The objective of this work was to evaluate their capacity to promote growth in seedlings of the same species under greenhouse conditions. The isolates that improved seedling growth were identified and characterized by their capacity to produce indoleacetic acid (IAA), solubilize phosphates and increase 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity. One hundred and five morphologically different strains were isolated, 15 of which promoted E. nitens seedling growth, significantly increasing the height (50%), root length (45%) as well as the aerial and root dry weight (142% and 135% respectively) of the plants. Bacteria belonged to the genus Arthrobacter, Lysinibacillus, Rahnella and Bacillus. Isolates A. phenanthrenivorans 21 and B. cereus 113 improved 3.15 times the emergence of E. nitens after 12 days, compared to control samples. Among isolated R. aquatilis, 78 showed the highest production of IAA (97.5±2.87 μg/ml) in the presence of tryptophan and the highest solubilizer index (2.4) for phosphorus, while B. amyloliquefaciens 60 isolate was positive for ACC deaminase activity. Our results reveal the potential of the studied rhizobacteria as promoters of emergence and seedling growth of E. nitens, and their possible use as PGPR inoculants, since they have more than one mechanism associated with plant growth promotion.

Role of phosphate solubilizing bacteria on rock phosphate solubility and growth of aerobic rice.

Use of phosphate-solubilizing bacteria (PSB) as inoculants has concurrently increased phosphorous uptake in plants and improved yields in several crop species. The ability of PSB to improve growth of aerobic rice (Oryza sativa L.) through enhanced phosphorus (P) uptake from Christmas island rock phosphate (RP) was studied in glasshouse experiments. Two isolated PSB strains; Bacillus spp. PSB9 and PSB16, were evaluated with RP treatments at 0, 30 and 60 kg ha-1. Surface sterilized seeds of aerobic rice were planted in plastic pots containing 3 kg soil and the effect of treatments incorporated at planting were observed over 60 days of growth. The isolated PSB strains (PSB9 and PSB16) solubilized significantly high amounts of P (20.05-24.08 mg kg-1) compared to non-inoculated (19-23.10 mg kg-1) treatments. Significantly higher P solubilization (24.08 mg kg-1) and plant P uptake (5.31 mg plant -1) was observed with the PSB16 strain at the highest P level of 60 kg ha-1. The higher amounts of soluble P in the soil solution increased P uptake in plants and resulted in higher plant biomass (21.48 g plant -1). PSB strains also increased plant height (80 cm) and improved root morphology in aerobic rice. The results showed that inoculation of aerobic rice with PSB improved phosphate solubilizing activity of incorporated RP.

Biogeochemical processes in the continental slope of Bay of Bengal: I. Bacterial solubilization of inorganic phosphate

Microorganisms play a vital role in the biogeochemical cycles of various marine environments, but studies on occurrence and distribution of such bacteria in the marine environment from India are meager. We studied the phosphate solubilizing property of bacteria from the deep sea sediment of Bay of Bengal, India, to understand their role in phosphorous cycle (and thereby the benthic productivity of the deep sea environment). Sediment samples were obtained from 33 stations between 10 degrees 36'N-20 degrees 01' N and 79 degrees 59' E-87 degrees 30' E along 11 transects at 3 different depths i.e. ca. 200 m, 500 m, 1000 m in each transect. Total heterotrophic bacterial (THB) counts ranged from 0.42 to 37.38 x 10(4) CFU g(-1) dry sediment weight. Of the isolates tested, 7.57% showed the phosphate solubilizing property. The phosphate solubilizing bacterial genera were Pseudomonas, Bacillus, Vibrio, Alcaligenes, Micrococcus, Corynebacterium and Flavobacterium. These strains are good solubilizers of phosphates which ultimately may play a major role in the biogeochemical cycle and the benthic productivity of the Exclusive Economic Zone (EEZ) of Bay of Bengal, because this enzyme is important for the slow, but steady regeneration of phosphate and organic carbon in the deep sea.