Quantitative evaluation of renal parenchymal perfusion using contrast-enhanced ultrasonography in renal ischemia-reperfusion injury in dogs

This study evaluated whether renal perfusion changes can be noninvasively estimated by using contrast-enhanced ultrasonography (CEUS) in renal ischemia-reperfusion injury and investigated the correlation between renal perfusion measured by CEUS and necrosis and apoptosis of renal tubular epithelial cells. In six dogs with experimentally induced renal ischemia-reperfusion injury, changes in time to peak intensity, peak intensity, and area under the curve were measured on CEUS. Peak intensity and area under the curve of the renal cortex began to decrease on day 1 (about 20% lower than baseline) and reached the lowest levels (about 50% of baseline) on day 4. They then gradually increased until day 10, at which time peak intensity was about 87% and area under the curve was about 95% of baseline; neither fully recovered. Both parameters were strongly correlated with the necrosis scores on histopathologic examination on day 4 (r = −0.810 of peak intensity and r = −0.886 of area under the curve). CEUS allowed quantitative evaluation of perfusion changes in acute renal ischemia-reperfusion injury, and CEUS results were correlated with renal tubular damage on histopathologic examination. Thus, CEUS could be a noninvasive, quantitative diagnostic method for determining progress of renal ischemia-reperfusion injury.

Value of combined utilization of urinary CysC and NAG in the diagnosis of renal tubules damage / 国际检验医学杂志

Objective To discuss the diagnostic value of combined utilization of urinary cystatin C (CysC) and β‐N‐acetyl‐glu‐cosaminidase (NAG) in renal tubules injury .Methods Urine samples were collected from 130 cases of patients with renal tubular damage (case group) and 100 cases of health individuals (normal control group) ,respectively .The concentration of CysC and the activity of NAG in urine were detected .Results The sensitivity ,specificity ,and positive predictive value of combined utilization of CysC and NAG in diagnosis of renal tubules damage were 85 .4% ,89 .6% and 82 .1% ,respectively .The concentration of CysC and the activity of NAG in case group after treatment were both significantly different from those before treatment (P<0 .05) .Conclu‐sion Combined utilization of urinary CysC and NAG has a significant value for the diagnosis and the therapeutic monitoring in re‐nal tubules injury .

A trying to evaluate acute renal tubular damage after ESWL / 대한비뇨기과학회지

Determination of urinary marker proteins was carried out trying to evaluate renal tubular damage as a part of renal injury caused by ESWL via EDAP LT-01+lithotiptor. All of twenty patients undergoing ESWL, suffered from unilateral, nonobstructive, infection-free, and previously untreated renal stone (s). Two urinary marker proteins, N-acetyl-beta-gluoosaminidase (NAG) and beta 2-micmglobulin (beta2-M) were monitored in all the patients 1 day before, 1 and 3 day (s) after ESWL using total 100 storage of 3 sessions which is average therapeutical dose. Urinary level or NAG (normal value; 0.29-7.23 U/L) was 1.66+/-1.31 1 day before, 1.97+/-1.57, 2.66+/-2.90 U/L 1 and 3 day (s) after ESWL respectively. beta2-M level (normal value <300 ug/L) was 142+/-137 1 day before. 133+/-100, 151+/-186 ug/L 1 and 3 day(s) alter ESWL, respectively. Consequently it was concluded that ESWL using average therapeutical dose (100 storage) via EDAP LT-01+ litholriptor can hardly develop renal tubular damage.