Chemical constituents of anti-oxidative activities from seeds of Jatropha curcas / 中草药

Objective: To study the chemical constituents with anti-oxidative activities from the seeds of Jatropha curcas. Methods: DPPH Radical scavenging assay was used for screening the active fractions. Using activity-guided- isolation, the active fractions were separated by column chromatography with silica gel, ODS, and Sephadex LH-20. The isolated compounds were then identified by spectral analyses (UV, IR, ESI-MS, HR-ESI-MS, 1H-NMR, 13C-NMR, and 2D-NMR). Anti-oxidative activities of all the isolated compounds were determined finally. Results: The n-butanol layer of ethanol extract from the seeds of J. curcas showed stronger activity and was studied further. Six compounds were isolated from this active layer and their structures were identified as jatrophasin A (3, 4, 4′, 5′-tetrahydroxyl- 5′-methoxyl-bisepoxylignan, 1), (±)-3, 3′- bisdemethylpinoresinol (2), 7-epi-sesamin-dicatechol (3), isoprincepin (4), β-sitosterol (5), and β-daucosterol (6). Conclusion: Compounds 3 and 4 show the strong, 1 and 2 show the medium anti-oxidative activities. Compound 1 is a new compound, named jatrophasin A, compounds 2, 3, and 4 are reported for the first isolation from the plants of Jatropha L.

A New Compound with Anti-oxidative Activity from Seeds of Jatropha curcas / 中草药·英文版

Objective To search for chemical constituents with anti-oxidative activity from seeds of Jatropha curcas.Methods DPPH radical scavenging assay was used to screen fractions or constituents with anti-oxidative activities.Active fractions were separated by varied chromatography and then identified on the basis of physiochemical properties and spectroscopic methods.Results The n-butanol layer of ethanol extract from the seeds of J.curcas showed stronger activity than other fractions and was studied further.A new compound was isolated from this active layer, and its structure was identified as jatrophasin A (3,4,4',5'-tetrahydroxyl-3'-methoxyl-bisepoxylignan, 1).It showed stronger anti-oxidative activity compared with resveratrol.Conclusion Compound 1 is a new compound which has never been reported with strong anti-oxidative activity.