Résumé
By reviewing the relevant literature in recent years, this paper analyzes the transformation and conduction of moxibustion physical signals, and explores the action mechanism of moxibustion. It is found that the different molecular targets activated by moxibustion photothermal signal are the keys to transform stimulation signal into biological signal. Photothermal signal transmits signal through neuroendocrine immune system and produces a series of biological reactions, thus regulating the functions of various systems of the body.
Sujets)
Rythme cardiaque , Système immunitaire , MoxibustionRésumé
Objective To study the expressions of Nodal in normal gallbladder,and gallbladders with cholelithiasis,cholecystitis and carcinoma;and to study the impact of inhibiting or promoting Nodal expressions in gallbladder carcinoma on the Smad2/4 pathway and epithelial-mesenchymal transition.Methods Immunohistochemistry was used to detect the expressions and distributions of Nodal protein in 30 normal gallbladders,96 simple cholecystitis/calculous cholecystitis specimens and 42 gallbladder carcinoma specimens.The mRNA and protein expressions of Nodal in normal and malignant gallbladdcr mucosal epithelium cells and breast cancer cells were detected by RT-PCR,western blotting and wound healing tests.The impact of activating agents and inhibitors on the expression levels of Nodal and its signaling pathway Smad2/4 and EMT-related proteins were analyzed.Results Immunohistochemistry showed that the positive rates of Nodal in the gallbladder cancer group was significantly higher than that in the gallbladder stone group and the normal gallbladder group.The results were 83.3％ (35/42),44.8％ (43/96),6.7％ (2/30) respectively (P＜ 0.01).RT-PCR and Western blotting showed the expressions of Nodal in gallbladder carcinoma cells were higher than normal gallbladder cells (P＜0.05).After using rhNodal to up regulate the Nodal expression,the Smad2 protein phosphorylation was promoted and the EMT associated proteins were up-regulated.After using the inhibitor SB431542 to suppress the Nodal expression,the Smad2 protein phosphorylation decreased and the EMT associated proteins were down-regulated.Conclusions The expression of Nodal was closely related to cell proliferation and metastasis in gallbladder carcinoma.Tumor progression was promoted via the smad2/4 pathway through epithelial-mesenchymal transition.
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The abnormal changes in the apoptosis activities are relevant to a number of diseases including the tumor genesis and development. Since the gene structure of NF-κB being cloned three decades ago, the investigation of the transcriptional factor has been improved over time. A series of target genes are revealed to be modulated, which participate in physiological and pathological activities, including inflammation, proliferation, differentiation,senescence, and apoptosis. This review is focused on the signaling network of cell apoptosis regulated by NF-κB, the molecular involved, as well as the environmental variants associated. Furthermore, as NF-κB is unusually up-regulated in diseases such as cancer, searching for its specific antagonists may prevent the occurrence of those diseases.
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The abnormal changes in the apoptosis activities are relevant to a number of diseases including the tumor genesis and development. Since the gene structure of NF-κB being cloned three decades ago, the investigation of the transcriptional factor has been improved over time. A series of target genes are revealed to be modulated, which participate in physiological and pathological activities, including inflammation, proliferation, differentiation,senescence, and apoptosis. This review is focused on the signaling network of cell apoptosis regulated by NF-κB, the molecular involved, as well as the environmental variants associated. Furthermore, as NF-κB is unusually up-regulated in diseases such as cancer, searching for its specific antagonists may prevent the occurrence of those diseases.
Résumé
Focal adhesion kinase(FAK),the non-receptor tyrosine kinase,has been implicated in regulating a number of fundamental biological signal transudation pathways that control cell proliferation,survival,adhesion,motility.Many researches have reported that FAK highly expressed in many cancers and involved in the process of cancer development,such as initial,progress,metastasis,multi-drug resistance of cancer.FAK has been the hotpot in cancer research,and it will be a new target in cancer therapy.