Résumé
Objective:To observe the relationship between the vascular endothelial growth factor (VEGF) and the survival of flap in the rat back which received radiotherapy. Methods: Forty Wistar rats were divided into four groups. A 3×4 cm~2 full thickness dorsal flap with pedicle was prepared at the posterior end. The skin flaps were given~(60)Co radiotherapy in the 2, 4, 6 day postoperation. Group A was given of VEGF in the third, fifth, seventh day. Group B was given a high quantity of VEGF. Group C was the control of Group A,Group D was the control of Group B. Control groups received normal saline. Results: Pathological observation revealed that the survival of flap and blood vessel lumen diameter of group A were higher than that of group B(P<0.05). Group A showed remarkable difference compared with group C(P<0.01). Group B showed remarkable difference compared with group D(P<0.05). Results of fluorescence staining and SDH examination showed that three times injection had better results than one time injection. Conclusion: Three times injection of VEGF has better effect on the viability of rat skin flap than one time injection.
Résumé
Objective To study the effect of vascular endothelial growth factor(VEGF) on the viability of skin flap treated with radiotherapy at different time after operation and clarify its enhancing effect on angiogenesis.Methods Fifty Wistar rat were randomly divided into 3,5,7,9 d groups and control group.A 3 cm?4 cm full thickness dorsal flap with the pedicle remaining attached at the posterior end in rats and were treated with 60Co radiotherapy at the 2nd,4th,and 6th day postoperatively.The total quantity was 12 Gy.In the experiment groups every rat was given VEGF 120 ng on the 3rd,5th,7th and 9th day.The rats in control group were given the same quality normal saline and radiotherapy.Pathological observation,fluorescence staining and sesuccinic dehyrogenase(SDH) assay were used to measure the blood vessel density,blood vessel diameter,microcirculation and cell vitality of skin flap.Results In 5 d and 7 d VEGF group,the number of average blood vessels and cells of the skin falp was increased compared with control group(P