Résumé
The aim of this study was to evaluate the physical and morphological characteristics of the sperm-rich fraction of jackass semen. To this end, 130 ejaculates from five Pêga jackasses were collected using an open model artificial vagina. The sperm-rich fraction was collected using the split-ejaculate method and assessed for the number of mounts/ejaculate, for physical and morphological characteristics of the semen and number of doses produced/ejaculate. It was observed that all characteristics evaluated differed among the five jackasses, except for the head defect rates. The mean values obtained for the jackass sperm-rich fraction collected were: number of mounts/ejaculate - 1.27; semen volume - 20.21mL; motility - 84.53%; vigor - 4.46; motility after dilution - 80.10%; sperm concentration/mL - 894.38 x 10 6; total sperm/ejaculate - 16.14 x 10 9; number of insemination doses/ejaculate (400x10 6 motile sptz) - 33.39; number of insemination doses/ejaculate (800 x 10 6 motile sptz) - 16.69; and percentage of normal sperm - 90.46%. Thus, in the present experiment the split-ejaculate method using an open artificial vagina worked well with the jackasses, and the sperm-rich fraction of the ejaculate of Pêga jackasses had high quality and sperm concentration, allowing its use for semen processing without reducing the number of insemination doses produced per ejaculate.(AU)
Foram coletados 130 ejaculados de cinco jumentos da raça Pêga, utilizando-se vagina artificial modelo aberta, com o objetivo de se avaliar as características físicas e morfológicas da fração rica em espermatozoides do ejaculado de asininos. Para tal, a fração rica em espermatozoides, composta pelos três primeiros jatos ejaculados, foi coletada e avaliada quanto ao número de montas/ejaculado, quanto às características físicas e morfológicas do sêmen, bem como quanto ao número de doses inseminantes produzidas/ejaculado. Observou-se que todas as características avaliadas diferiram entre os cinco reprodutores avaliados, com exceção do percentual de defeitos de cabeça. Os valores médios obtidos da coleta da fração rica do ejaculado de jumentos foram: número de montas/ejaculado - 1,27; volume de sêmen - 20,21mL; motilidade - 84,53%; vigor - 4,46; motilidade pós-diluição - 80,10%; espermatozoides/mL - 894,38 x 10 6; espermatozoides/ejaculado - 16,14 x 10 9; número de doses inseminantes/ejaculado (400x10 6 sptz móveis) - 33,39; doses inseminantes/ejaculado (800 x 10 6 sptz/móveis) - 16,69; e percentual de espermatozoides normais - 90,46%. Assim, no presente experimento, observou-se boa aceitação dos reprodutores à coleta fracionada utilizando-se a vagina artificial aberta, sendo a fração rica do ejaculado de jumentos da raça Pêga caracterizada por alta qualidade e concentração espermática, o que viabilizou sua utilização para o processamento do sêmen, sem prejuízos quanto ao número de doses inseminantes produzidas/ejaculado.(AU)
Sujets)
Animaux , Mâle , Equidae , Sperme , Numération des spermatozoïdes/médecine vétérinaire , Mobilité des spermatozoïdes , Prélèvement d'organes et de tissus/méthodesRésumé
This study was designed to analyze the sperm kinematic and morphometric subpopulations in the different fractions of the ejaculate in normozoospermic men. Ejaculates from eight normozoospermic men were collected by masturbation in three fractions after 3-5 days of sexual abstinence. Analyses of sperm motility by computer-assisted sperm analysis (CASA-Mot), and of sperm morphometry by computer-assisted sperm morphometry analysis (CASA-Morph) using fluorescence were performed. Clustering and discriminant procedures were performed to identify sperm subpopulations in the kinematic and morphometric data obtained. Clustering procedures resulted in the classification of spermatozoa into three kinematic subpopulations (slow with low ALH [35.6% of all motile spermatozoa], with circular trajectories [32.0%], and rapid with high ALH [32.4%]), and three morphometric subpopulations (large-round [33.9% of all spermatozoa], elongated [32.0%], and small [34.10%]). The distribution of kinematic sperm subpopulations was different among ejaculate fractions (P < 0.001), with higher percentages of spermatozoa exhibiting slow movements with low ALH in the second and third portions, and with a more homogeneous distribution of kinematic sperm subpopulations in the first portion. The distribution of morphometric sperm subpopulations was also different among ejaculate fractions (P < 0.001), with more elongated spermatozoa in the first, and of small spermatozoa in the third, portion. It is concluded that important variations in the distribution of kinematic and morphometric sperm subpopulations exist between ejaculate fractions, with possible functional implications.
Résumé
Com o objetivo de se estudar o efeito do tipo de envase sobre a criopreservação do sêmen asinino, coletado de forma fracionada, utilizou-se o sêmen de cinco jumentos da raça Pêga, submetido a um protocolo de congelamento envolvendo um diluidor e duas formas de envase, flatpacks ou palhetas de 0,55mL, tendo como ponto final a avaliação dos parâmetros seminais in vitro após o descongelamento do sêmen. Os resultados de motilidade espermática para as amostras envasadas em flatpacks ou em palhetas foram de 24,67% e 35,34%, no momento do descongelamento; de 13,39% e 25,83%, 60 minutos após o descongelamento; e de 7,86% e 13,33%, aos 120 minutos após o descongelamento, respectivamente. Já os valores para o vigor espermático foram de 2,55 e 3,33, no momento do descongelamento; de 1,57 e 2,48, após 60 minutos do descongelamento; e de 1,11 e 1,67, após 120 minutos do descongelamento, na mesma ordem anterior. As características seminais diferiram entre os jumentos, evidenciando uma grande variação individual. O percentual de ejaculados aprovados (motilidade ≥30%, vigor ≥3) foi influenciado pelo reprodutor e pelo tipo de envasamento, estando o melhor resultado (P<0,05), de 86,21%, associado ao sêmen envasado em palhetas, quando comparado ao valor de 56,67%, obtido para o sêmen envasado em flatpacks.
The effect of the type of package on the cryopreservation of jackass semen was studied, using the sperm-rich fraction of five jackasses and a special cryopreservation semen protocol, related to one extender and two different packages, FlatPacks or straws. The characteristics of the in vitro semen were evaluated after thawing. The motility results were 24.67% and 35.34% after thawing; 13.39% and 25.83% 60 minutes after thawing and 7.86% and 13.33%, 120 minutes after thawing. The vigor results were 2.55 and 3.33 after thawing, 1.57 and 2.48 60 minutes after thawing and 1.11 and 1.67 120 minutes after thawing, for FlatPacks or straws packages, respectively. The jackasses used were different regarding seminal characteristics, with great individual variation. The approved post-thaw viability (motility ≥ 30%, vigor ≥ 3) was affected by jackass and type of package, with the best results (P<0.05) for semen packed in straws (86.21%), compared to semen packed in FlatPacks (56.67%).