Morphology accuracy evaluation of direct composite occlusal veneer using two types of modified stamp-technique / 北京大学学报(医学版)

OBJECTIVE@#To evaluate the morphology accuracy of direct occlusal veneer using two types of modified stamp-technique, comparing the Results of two types of stamp and different composite resin.@*METHODS@#Model scanner was used to get the original data from the standard resin teeth in plastic model. Two types of stamps were made: solid silicon stamp and transparent silicon stamp. In the study, 54 resin tooth were randomly divided into 9 groups: Groups 1-8 were restored by direct composite resin, using general and bulk-filled composite resin in occlusal veneer (1 mm or 2 mm) with two types of stamp-technique (the solid silicon, and the transparent silicon), and the control group was restored by indirect way using CAD/CAM composite resin restoration by biocopy technique to mimic the original shape of the teeth. After the resin teeth were prepared for occlusal veneer, the direct and indirect composite resins were restored. After the restoration was finished, the data were obtained again by the same model scanner in the plastic model. 3D comparisons were made using the original and restoration data, the average difference and the root mean square of difference (RMS) were recoded from the software. The RMS of all the groups were analyzed using one way ANOVA (α=0.05).@*RESULTS@#The RMS of occlusal surface in solid silicon stamp group was (0.136±0.031) mm, in transparent silicon group was (0.130±0.024) mm, and in control group was (0.130±0.009) mm. There were no significant difference. While the RMS of buccal/lingual surface in solid silicon stamp group was the smallest [(0.135±0.020) mm, P < 0.05], and in transparent silicon group it was (0.147±0.021) mm, and control group (0.153±0.014) mm. The general composite resin using the two types of stamp had the largest occlusal RMS in 2 mm occlusal veneer, which were significantly larger than control group (P < 0.05).@*CONCLUSION@#The direct occlusal veneer using two types of modified "stamp-technique" had relatively good morphology accuracy. Direct occlusal veneer using bulk-filled composite resin made with two types of stamp technique had even more accurate morphology than using general composite resin. The solid silicon stamp had a better morphology accuracy in buccal/lingual surface than the transparent silicon stamp.

Predictive value of STAMP in Health Information System in children with critical congenital heart disease / 中国胸心血管外科临床杂志

@#Objective    To analyze the perdictive value of Screening Tool for the Assessment of Malnutrition in Pediatrics (STAMP) for malnutrition or postoperative complications in children with critical congenital heart disease (CHD). Methods    A total of 875 children with critical CHD who were hospitalized in West China Hospital, Sichuan University form August 2019 to February 2021, including 442 males and 433 females with a median age of 30 (12, 48) months, were assessed by STAMP in Health Information System. Clinical data of postoperative complications were collected. Results    (1) Based on World Health Organization Z-score as gold standard, 24.5% had malnutrition risk, and 34.3% were diagnosed with malnutrition. According to STAMP, the children were with medium malnutrition risk of 37.9% and high malnutrition risk of 62.1%. There was a statistical difference of incidence rate of malnutrition and detection rate of STAMP malnutrition risk in gender, age, ICU stay or length of mechanical ventilation (P<0.05); (2) with the optimal cut-off point of 5.5 in STAMP for malnutrition, the sensitivity, specificity, positive predictive value, negative  predictive value and area under the curve (AUC) were 68.3%, 84.3%, 48.1%, 88.3% and 0.82, respectively; (3) 12.0% of the children were with postoperative complications; (4) with the optimal cut-off point of 5.5 in STAMP for postoperative complications, the sensitivity, specificity, positive predictive value, negative predictive value and AUC were 83.8%, 73.1%, 18.8%, 99.1% and 0.85, respectively. Conclusion    Children with critical CHD have a higher incidence of malnutrition risk and postoperative complications. STAMP has a good perdictive value for malnutrition or postoperative complications, however, the sensitivity and specificity of STAMP are affected by the gold standard or the cut-off point.

DC-STAMP and tace levels are higher in patients with periodontitis

Abstract Although periodontitis is one of the commonest infectious inflammatory diseases in humans, the mechanisms involved with its immunopathology remain ill understood. Numerous molecules may induce inflammation and lead to bone resorption, secondary to activation of monocytes into osteoclasts. TACE (TNF-α converting enzyme) and DC-STAMP (dendritic cell-specific transmembrane protein) appear to play a role on bone resorption since TACE induces the release of sRANKL (soluble receptor activator of nuclear factor kappa-β ligand) whereas DC-STAMP is a key factor in osteoclast induction. The present study evaluated the levels of TACE and DC-STAMP in patients with and without periodontitis. Twenty individuals were selected: 10 periodontally healthy participants undergoing gingivectomy for esthetic reasons and 10 diagnosed with periodontitis. Protein levels of such molecules in gingival tissue were established using Western blotting. Protein levels of both TACE and DC-STAMP were higher in the periodontitis group than in the control group (p<0.05; Student t-test). In conclusion, TACE and DC-STAMP protein levels are elevated in patients with periodontitis, favoring progression of bone resorption.

Resumo Apesar de a periodontite ser uma das doenças infecto inflamatórias humanas mais comuns, os mecanismos que conduzem à imunopatologia não estão bem definidos. Inúmeras moléculas induzem atividade inflamatória que levam à perda óssea. Para que haja a reabsorção óssea, células monocíticas são ativadas e se transformam em osteoclastos. As moléculas TACE (Enzima conversora de TNF-α) e DC-STAMP (Proteína transmembrana específica de célula dendrítica) parecem atuar no processo de reabsorção óssea uma vez que a TACE induz a liberação de sRANKL (ativador do receptor do fator nuclear kappa-β ligante solúvel), enquanto a DC-STAMP é um fator chave na indução dos osteoclastos. Diante disso, o presente estudo avaliou a expressão gênica das moléculas TACE e DC-STAMP em pacientes com e sem periodontite uma vez que o papel destas moléculas no curso do desenvolvimento da periodontite ainda é pouco explorado. Foram selecionados 20 indivíduos, sendo 10 com saúde periodontal e com indicação para remoção de tecido gengival por motivos estéticos e 10 pacientes com periodontite. As análises da expressão das moléculas no tecido gengival foram realizadas por meio de western blotting. Os níveis proteicos tanto de TACE quanto de DC-STAMP, foram maiores nos tecidos do grupo com periodontite em comparação aos do grupo controle (p<0.05; Student' t-test). Portanto, os dados demonstram que a expressão protéica das moléculas TACE e DC-STAMP estão elevados em pacientes com periodontite, favorecendo a progressão da reabsorção óssea nesta patologia.

The Effects of Stamping or Printing on Tablet or Capsule Readability / 医薬品情報学

Background: With the marked changes occurring in the medical field, such as rapid population aging, the frequency of one‐dose package use by medication‐dispensing services is increasing.　Pharmaceutical companies promote the development of pharmaceutical products that can be identified based on their color, size, and imprinted information to reduce one‐dose packaging errors and difficulties in tablet identification.　However, there have not been any studies about the effectiveness of such measures for aiding the identification of tablets in the clinical setting.　Therefore, we examined the effects of imprinting on the ease of identification of tablets and capsules.Methods: The study was conducted over a 3‐month period and involved 39 pharmacists aged under 40.　The times they needed to transcribe the characters imprinted on each tablet/capsule were measured.Results: The time needed to identify a tablet was significantly shortened by kana printing (p<0.01), whereas more time was required to identify a capsule when the text color was similar to that of the capsule.　The observed transcription errors included ‘inaccurately transcribing alphanumeric characters' and ‘omitting units or other information.'Discussion: These results suggest that kana printing is effective at increasing the ease of tablet/capsule identification, which is also affected by the color of the printed text.

Expressions of TXNIP,STAMP2 and GATA3 and the changes of serum oxidative stress indexes in diabetic patients with breast cancer / 中国糖尿病杂志

Objective To measure the expressions of TXNIP,STAMP2 and GATA3 in diabetes patients with breast cancer and to determine the changes of oxidative stress indexes before and after surgery.Methods The expression levels of TXNIP,STAMP2 and GATA3 mRNA in cancer tissues and adjacent normal tissues were measured in 45 diabetes patients with breast cancer.The levels of serum oxidative stress indexes including MDA,MPO,SOD and TAC were detected and compared before and after surgery.Results The expressions of TXNIP,STAMP2 and GATA3 mRNA and their protein levels in cancer tissues were lower than those in adjacent normal tissues (P<0.05).The positive expression rate of the three indexes were 60%,66.7% and 73.3%,higher than that of TXNIP protein with 2.2% in the adjacent normal tissues(P<0.05).The TXNIP,STAMP2 and GATA3 protein positive rate of breast cancer tissue were related to differentiation,lymph node metastasis and clinical grades (P<0.05).Serum MDA and MPO levels increased first and then decreased.SOD and TAC showed a trend of decrease first and then increase.The turning point is 6 d after surgery.Conclusion TXNIP,STAMP2,GATA3 and serum oxidative stress indicators may be the indicators for diagnosis and treatment of breast cancer in diabetes patients.

An association study of six-transmembrane protein of prostate 2 gene with metabolic index and tumour necrosis factor in Uygur population / 中华内科杂志

Objective To explore the relationship between genetic polymorphisms of sixtransmembrane protein of prostate 2 (STAMP2) and metabolic index,TNFα in Xinjiang Uygur population.Methods STAMP2 gene functional regions were sequenced in Uygur Xinjiang population diagnosed as metabolic syndrome.Patients were divided into the following three groups by their TNFα concentration:the high level group(TNFα≥7.95 μg/L,n =313),the moderate level group(TNFα ＞5.34-＜7.95 μg/L,n =268)and the low level group(≤5.34 μg/L,n =313).The selected representative variations were genotyped by TaqMan-PCR in 894 Uygur individuals.The association of the genetic variations of STAMP2 gene with metabolic index and TNFα was analyzed.Results Three representative variations were genotyped,including rs8122,rs1981529 and rs34741656.The genotype distribution and allele frequencies of rs8122and rs1981529 were statistically different among the three groups (P ＜ 0.05),while no difference was observed with rs34741656(P ＞0.05).By ANOVA analysis,statistical difference was showed between the rs1981529 polymorphism AA and AG in the concentration of TNFα(P ＜ 0.05).None of the polymorphisms was significantly associated with TC,HDL-C,LDL-C and TG (P ＞ 0.05).Conclusion Two STAMP2 gene polymorphisms,rs8122 and rs1981529 are associated with the concentration of TNFα in Xinjiang Uygur population.

Investigations on nutritional status and nutritional risk in hospitalized pediatric patients / 临床儿科杂志

Objectives To validate the Screening Tool for the Assessment of Malnutrition in Pediatrics (STAMP) in hospitalized pediatric patients, and to assess the nutritional status and nutritional risk using STAMP. Methods the nutritional status of hospitalized pediatric patients was investigated by adopting STAMP. The STAMP score≥4 was deifned as a high standard of nutritional risk. Results Among 1 506 hospitalized children, children with high nutritional risk accounted for 26.56%. The overall prevalence of malnutrition and stunting was 19.12%and 10.16%, respectively. There was signiifcant diffe-rence in stunting among each department (P<0.05), which was the highest in department of internal pediatrics. The proportion of patients receiving nutritional support was 10.09%. Among them, the rate of parenteral and enteral nutrition support was 7.84%and 2.92%, respectively. The ratio of parenteral nutrition to enteral nutrition was 2.68:1. After intervention to hospitalized pa-tients with high nutritional risk, the overall prevalence of high nutritional risk decreased from 26.56%at admission to 21.71%at discharge (P<0.05). Conclusions STAMP can objectively relfect the possible malnutritional risk in hospitalized pediatric patients. The nutrition condition of the pediatric patients can be improved through nutritional risk management.

Nutritional assessment and intervention of hand-foot-mouth disease in children / 临床儿科杂志

Objectives To conduct nutritional risk assessment analysis of hand-foot-mouth disease in children and to explore rational and feasible nutritional intervention. Methods The information of children with hand-foot-mouth disease in three hospitals in Guilin area was collected by ifxed-spot continuous sampling. Inquiry investigations, physical mea-surements combined with clinical diagnosis were undertaken, and nutritional risk assessment and management were con-ducted by the malnutrition screening tool of children (STAMP scoring). Those with STAMP scores ≥4 were considered at high nutritional risk. Nutritional risk reassessment was conducted on children with high nutritional risk after nutritional intervention and management. Results A total of 192 children with hand-foot-mouth disease were selected. The occur-rence rate of high nutritional risk was 68.75%(132/192). The hospitalization time of patients with high nutritional risk was prolonged and the hospitalization expense was increased apparently. The nutritional support treatment and nutritional edu-cation were undertaken on the children with high nutritional risk. The incidence of high nutritional risk from admission to discharge decreased from 68.75%to 36.98%(P<0.05). The clinical outcomes of those children accepting nutritional inter-vention were signiifcantly improved (P<0.05). Conclusions The occurrence and prognosis of hand-foot-mouth disease is closely related to the nutritional status of children, so nutritional risk assessment, nutritional intervention and management should be performed as early as possible.

Nocturnal Penile Tumescence Monitoring with Stamps / 대한비뇨기과학회지

Nocturnal penile tumescence monitoring has gained wide acceptance in differential diagnosis of impotence. We developed a modified stamp technique which use a strip of stamps, each 2.2cm by 2.5cm, and the vinyl plaster for snugger wrapping and sealing of stamps around the penile shaft. This is a simple, time-saving and useful screening test for organic impotence.