Résumé
Oestrogen is produced in sizable quantities in the testis, as well as the brain. Progesterone enhances libido, sperm count, improves mood, and keeps weight down while increasing muscle mass. This study investigated some of the effects of oestrogen and progesterone on the testis of adult male Wistar rats. Twenty (20) adult male wistar rats were randomly assigned into four groups (A-D) (n=5) and drugs were administered to the rats as follows: Group A received 1ml of distilled water per day, group B received 5 mg/kg b.w. of Stilbestrol per day, group C received 0.5 mg/kg b.w. of Lynestrenol per day while group D received 5 mg/kg body weight of Stilbestrol and 0.5 mg/kg b.w. of Lynestrenol. Histological, testicular histomorphometry, hormonal and semen parameters were observed. Histological evaluations for group (B-D) showed elongated seminiferous tubules, degeneration of the basement membrane, severe thinning of sertoli cells, reduced number of spermatogenic cells, wide interstitial space and scanty leydig cells. Biochemical analyses revealed a significant increase (P<0.05) in serum follicle stimulating hormone (FSH) levels in the experimental groups (B-D) when compared to the control group. Semen analysis showed that there was a significant reduction (P<0.05) in Sperm Motility and Life and Death ratio (L/D) in all experimental groups while significant decrease (P<0.05) in sperm morphology was observed in groups B and C while no significant differences (P>0.05) was observed in sperm count in the treatment groups compared with control group. These findings suggest that Stilbestrol and lynestrenol administrations had deleterious effects on testicular cell morphology.
Résumé
AIM: To establish a dysmenorrhea model in mice. METHODS: The mice were given with some kinds of oestrogens once a day for 3-25 days. On the last day, the mice were injected intraperitoneally with oxytocin and the number of twisting body was recorded to evaluate the intensity of dysmenorrhea. The optimum conditions to establish the model was analysed statisticly. RESULTS: The optimum oestrogens was stilbestrol. Stibestrol should be given for 12-15 days. The regression equation of the dose-effect curve of stibestrol was Y = 0.03±0.04 X, r = 0. 9688. The optimum dosage of oxytocin was 20 U·kg-1. The dysmenorrhea model in mice could be preserved for about 7 days. 90% of the twisting body reactions concentrated in 0-30 minutes after oxytocin was given. The effect of oxytocin (20 U·kg-1) had significent difference with that of prostaoglantin (1.3 mg·kg-1). The test of uterus in vivo showed that stilbestrol could increase the uterine contraction frequency and strengthen the contractility. The dysmenorrhea model in mice was testified by some anti- dysmenorrhea drugs. CONCLUSION: Compared with the hypodermic implantation in rats, the dysmenorrhea model in mice was simple, reliable, economical and testifiable, etc.
Résumé
AIM To establish a dysmenorrhea model in mice. METHODS The mice were given with some kinds of oestrogens once a day for 3～25 days. On the last day, the mice were injected intraperitoneally with oxytocin and the number of twisting body was recorded to evaluate the intensity of dysmenorrhea. The optimum conditions to establish the model was analysed statisticly. RESULTS The optimum oestrogens was stilbestrol. Stibestrol should be given for 12～15 days. The regression equation of the dose effect curve of stibestrol was =0 03?0 04 X, r =0 9688. The optimum dosage of oxytocin was 20 U?kg -1 . The dysmenorrhea model in mice could be preserved for about 7 days. 90% of the twisting body reactions concentrated in 0～30 minutes after oxytocin was given. The effect of oxytocin (20 U?kg -1 ) had significent difference with that of prostaoglantin (1 3 mg?kg -1 ). The test of uterus in vivo showed that stilbestrol could increase the uterine contraction frequency and strengthen the contractility. The dysmenorrhea model in mice was testified by some anti dysmenorrhea drugs. CONCLUSION Compared with the hypodermic implantation in rats, the dysmenorrhea model in mice was simple, reliable, economical and testifiable,etc.