Etiological & Microbiological Profile Of Infective Keratitis - A Hospital based study

INTRODUCTION: Microbial keratitis is a potentially serious corneal infection and a major cause of visual impairment worldwide. A conservative estimate of the number of corneal ulcers occurring annually in the developing world alone is 1.5-2 million. Permanent visual dysfunction has been reported in a significant proportion of patients in both developing and developed countries. Srinivasan et al comment that ulceration of the cornea in south India 'is a blinding disease of epidemic proportions. AIM: To study epidemiological, etiological & microbiological factors associated with the patients with infective keratitis attending the hospital. MATERIALS & METHODS: A Retrospective study of the patients who attended Ophthalmology Dept. of Gayatri Vidhya Parishad Instirtute of Health Care & Technology Marikavalasa Visakhapatnam for Infectious Keratitis .A total of 83 corneal scrapings from patients presenting with infective keratitis were evaluated from Dec2014 to Jan 2018. Scrapings were subjected to Gram stain KOH preparation and culture for bacterial and fungal pathogens on suitable media. RESULTS: About 55% of the patients with corneal ulcer presenting to this hospital were inflicted with trauma prior to the onset of keratitis.Injury with vegetable matter followed by injury with wooden particles and stones were the major cause of corneal insult. Microbiologicaletiology was established in 90% of cases (75 of 83cases). Out of 75 positive cultures 64% were bacterial and 36 % were fungal. Among the bacteria most common organism grown wasPseudomonas, Staph aureus followed by coagulase negative staphylococcus. Among Fungal isolates Fusarium followed by Aspergilluswere seen. CONCLUSIONS: In our study trauma with vegetable matter was the most common causative factor, followed by wooden particles and stones. This may be because most of the patients are agricultural laborers. Males are more commonly affected than females. Bacterial ulcers are slightly more common than fungal& Pseudomonas and Aspergillus were the most common pathogens.

Antibiotic sensitivity pattern of causative bacterial pathogens responsible for corneal ulcer.

Introduction: Number of blind people in the world is 45 million. Out of which 5.4 million blind people are in our country. Corneal ulcer is a major cause of blindness throughout the world. About10% cases of blindness are due to corneal ulcer. Aim:To detect antibiotic sensitivity Pattern of causative bacteria responsible for corneal ulcer. Material and mathod: 150 samples were collected during period of 1 year from clinically diagnosed cases of corneal ulcer at SSG Hospital, Baroda.Results: Total 150 samples were tested for antibiotic sensitivity pattern and among them Gram positive bacteria showed highest sensitivity to ciprofloxacin and among Gram negative bacteria showed highest sensitivity to ciprofloxacin, gatifloxacin and amikacin while pseudomonas showed highest sensitivity to imepenem.

Epidemiological and microbiological profile of infective keratitis in Ahmedabad.

Context: Study of patients attending tertiary care ophthalmology institute at Ahmedabad. Aims: To study the microbiological etiology and epidemiological factors associated with suppurative keratitis. Settings and Design: A total of 150 corneal scrapings were evaluated from patients presenting with corneal ulcers at a tertiary ophthalmology center, Ahmedabad from July 2007 to June 2008. Materials and Methods: Scrapings were subjected to Gram stain, potassium hydroxide preparation and culture for bacterial and fungal pathogens. Socio-demographic data and risk factors were recorded. Results: Ninety percent (135/150) people with corneal ulcers had trauma as predisposing factor for keratitis. Trauma due to wooden objects was the leading cause (46/135) followed by vegetable matter and stone injury (23/135). Microbial etiology was established in 59.3% (89/150) of scrapings. Out of 89 positive isolates, 65.1% (58/89) were bacterial while 34.9% (31/89) were fungal. Among the bacterial isolates, 60.3% (35/58) were Gram-positive cocci while 39.7% (23/58) were Gram-negative bacilli. The most common bacterial isolate was Staphylococus aureus (32.7%, 19/58) followed by coagulase-negative Staphylococci (25.8%, 15/58) and Pseudomonas (18.9%, 11/58). Among the 31 fungal pathogens, Aspergillus species was the most common (35.4%11/31), followed by Fusarium species (22.5%, 7/31). Conclusion: Trauma with wooden material is the most common predisposing factor for suppurative keratitis. Males were more affected than females. Bacterial ulcers were more common than fungal in areas in and around Ahmedabad. Staphylococcus aureus and Aspergillus were the commonest bacterial and fungal isolates respectively. Geographical variation persists in microbial etiology of suppurative keratitis.