Chinese expert consensus on clinical application of irradiated blood / 中国输血杂志

Transfusion associated graft versus host disease (TA-GVHD) is a serious transfusion adverse reaction. It is widely believed that transfusion of irradiated blood is the most effective method to prevent TA-GVHD. There are some problems in the application of irradiated blood in China, such as slow development, insufficient understanding, and non-standard application. This consensus was completed after in-depth discussion by experts in the fields of transfusion medicine, hematology, and pediatrics in China. The Writing Group mainly discuss key technical points and clinical applications related to irradiated blood, and proposes 23 related recommendations. This consensus aims to standardize and promote the rational use of irradiated blood, minimize the risk of TA-GVHD in recipients and lay the foundation for developing more standardized guidelines in the future.

Effect of 60 Co γ-ray irradiation on the quality of leukocyte-filtrated red blood cells suspension / 新乡医学院学报

Objective To investigate the effect of 60Co γ-ray irradiation on the quality of theleukocyte-filtrated red blood cells suspension.Methods Thirty bags of leukocyte-filtrated red blood cells were randomly divided into irradiation group(n =15) which was given 60Co γ-ray irradiation and control group(n =15) which was not given 60Co γ-ray irradiation.The content of extracellular K +,Na +,free hemoglobin (FHb),adenosine triphosphate (ATP) and 2,3-diphosphoglyceric acid (2,3-DPG) and pH value of the leukocyte-filtrated red blood cells were detected in the two groups.Results The content of K+,Na+,FHb,ATP,2,3-DPG and pH value in irradiation group was (5.20 ± 0.37) mmol · L-1,(140.22 ± 0.05) mmol · L-1,(0.44 ±0.04) mg· L-1,(5.18 ± 0.13)μmol· L-1,(3.34±0.30) mmol· L-1 and 6.85±0.02;the content of K +,Na +,FHb,ATP,2,3-DPG and pH value in the control group was(5.00 ± 0.40)mmol · L-1,(140.47 ± 0.83)mmol · L-1,(0.42 ± 0.08) mg · L-1,(5.22 ± 0.06) μmol · L-1,(3.56 ± 0.52) mmol · L-1 and 6.84 ± 0.05;there was no statistic difference in the content of K +,Na +,FHb,ATP,2,3-DPG,and pH value between the two groups(t =1.93,-1.67,-1.64,-2.02,1.77,1.82;P ＞ 0.05).Conclusion 6o Co γ-ray irradiation of the leukocyte-filtrated red blood cells can not effect the activity and function of red blood cells.

Anti-CD3 Antibody Ameliorates Transfusion-Associated Graft-Versus-Host Disease in a Chemotherapy-Based Mouse Model With Busulfan and Fludarabine

ABSTRACT To establish a transfusion-associated graft-versus-host disease (TA-GVHD) mouse model with busulfan and fludarabine for effective treatment evaluation. BALB/c (H-2d) mice were injected with busulfan (15 mg/kg) and fludarabine (30 mg/kg) twice a day for 4 days. The mice were transfused with 106 T cell-depleted bone marrow (TCD-BM )and cells in different groups 3 days after chemotherapy: syngeneic BALB/c, MHC minor mismatch DBA/2 (H-2d), or MHC major mismatch C57BL/6(H2-b). Recipient BALB/c mice were injected with either blood only or blood+splenocyte. TA-GVHD was monitored in terms of body weight loss, clinical scores, and survival. Dexamethasone (50 mg/kg), cyclophosphamide (50 mg/kg), cyclosporine A (30 mg/kg), and anti-CD3 (1 mg/kg) were injected to each group to examine the treatments. Blood transfusion alone is insufficient to induce TA-GVHD in a chemotherapy-based mouse model. A MHC-mismatched TA-GVHD model can be induced by splenocyte and blood transfusion. This MHC-mismatched TA-GVHD model was resistant to dexamethasone treatment. Treatment based on anti-CD3 monoclonal antibody slightly ameliorated TA-GVHD. Treatment effectiveness was associated with T-cell depletion following activation by anti-CD3. Busulfan and fludarabine chemotherapy regimen can be used to establish a TA-GVHD mouse model. Anti-CD3 monoclonal antibody is a potential alternative to treat TA-GVHD.