RÉSUMÉ
A field experiments was conducted on sandy loam soil during 2016 -17 and 2017 -18 at M.G. chitrakoot Gramodaya Vishwavidyalaya, Chitrakoot, Satna (M.P.) to find out the most productive and profitable mustard based cropping system under different moisture conservation practices. Treatment consisted three cropping systems viz. Sesame –mustard, green gram-mustard and urdbean –mustard and four moisture conservation practices (no irrigation, life saving irrigation, straw mulch @4 t/ha and kaolin 5% spray). Results revealed that on pooled basis of two years mulching @4 t/ha produced significantly higher grain yield of sesame (512 kg/ha), green gram (1023 kg/ha) urdbean (2349 kg/ha) and mustard (2558kg/ha) than no irrigation while numerical enhancement were recorded under life saving irrigation and kaolin 5 % spray treatment in seed yield of sesame, green gram, urdbean and mustard crop. The maximum mustard equivalent grain yield of 3759 kg/ha, system production efficiency of 33.52 kg/ha/day and system profitability of 339.00 Rs/ha/day was recorded under green gram – mustard system. Among the moisture conservation practices, straw mulch treatment had highest mustard equivalent grain yield of 4021 kg/ha, production efficiency of 35.83 kg/ha/day and system profitability (35.83 Rs/ha/day). The gross returns (Rs 182764/ha), net returns (Rs 139644/ha) and benefit: cost ratio (3.24) were maximized under green gram- mustard system x straw mulch @4 t/ha treatment on pooled basis.
RÉSUMÉ
An experiment was conducted on urdbean during kharif 2021 at Instructional Farm, College of Agriculture, Ummedganj, Kota (Rajasthan). The experiment comprised of 10 treatments viz. (Control, 75% RDF, 100% RDF, 75% RDF + Rhizobium @ 600 g ha-1 seed inoculation, 75% RDF + PSB @ 600 g ha-1 seed inoculation, 75% RDF + Rhizobium @ 600 g ha-1 + PSB @ 600 g ha-1, 75% RDF + 0.1% FeSO4 + 0.5% ZnSO4 at pre flowering and pod formation stage, 75% RDF + 0.1% FeSO4 + 0.5% ZnSO4 at pre flowering and pod formation stage + Rhizobium @ 600 g ha-1, 75% RDF + 0.1% FeSO4 + 0.5% ZnSO4 at pre flowering and pod formation stage + PSB @ 600 g ha-1, 75% RDF + 0.1% FeSO4 + 0.5% ZnSO4 at pre flowering and pod formation stage + Rhizobium 600 g ha-1+ PSB @ 600 g ha-1) was carried out in randomized block design with three replications. Significantly higher plant height at 60 DAS (25.28 cm) and at harvest (34.43 cm), nodules plant-1 (29.22) at 45 DAS, nodule fresh weight (86.31 mg plant-1) at 45 DAS, chlorophyll content (3.19 mg g-1) at 45 DAS and dry matter accumulation (2.71, 5.94 and 9.99 g plant-1) at 30, 60 DAS and at harvest were recorded with application of 75% RDF + 0.1% FeSO4 + 0.5% ZnSO4 at pre flowering and pod formation stage + Rhizobium 600 g ha-1+ PSB @ 600 g ha-1 in urdbean over rest of the treatments. Among these treatments, application of 75% RDF + 0.1% FeSO4 + 0.5% ZnSO4 at pre flowering and pod formation stage + Rhizobium 600 g ha-1+ PSB @ 600 g ha-1 produced maximum seed yield (1269.30 kg ha-1) of urdbean.
RÉSUMÉ
Aphids are one of the most devastating pests, affecting the potential yield and quality ofBrassica juncea. In the current study, we have attempted to pyramid two transgenic lines containing chickpea lectin (CHPL, P1) and urdbean protease inhibitor (UPI, P2) in each under the phloem specific rolC promoter, through conventional breeding approach. In the derived F2 population, both lectin and protease inhibitor genes were segregating in a 9:3:3:1 ratio (p-value: 0.81), indicative of a single copy of the transgenes in the parents. Furthermore, the parental, as well as pyramided progenies were evaluated for their potential resistance to aphids in terms of mortality and natality. The lines containing both the transgenes were found to be superior over single gene transgenics as a higher mortality rate (96%) was found in F2on the 9th day as compared to single gene transgenics (86% and 80% in P1 and P2 respectively). A significant decrease in the number of nymphs was observed in P1 and P2 but most in F2 plants as almost 43, 32.08, and 107.5 times decrease in the number of nymphs was found in P1, P2, and F2 individuals over control. Expression profiling was done to see if there was any impact of gene pyramiding on the expression pattern of both transgenes before and after aphid treatment, and no significant changes were observed, indicating constitutive expression of transgenes in pyramided lines also. In conclusion, pyramided lines were found to be promising and were superior for aphid resistance.
RÉSUMÉ
Aphids are one of the most devastating pests, affecting the potential yield and quality ofBrassica juncea. In the current study, we have attempted to pyramid two transgenic lines containing chickpea lectin (CHPL, P1) and urdbean protease inhibitor (UPI, P2) in each under the phloem specific rolC promoter, through conventional breeding approach. In the derived F2 population, both lectin and protease inhibitor genes were segregating in a 9:3:3:1 ratio (p-value: 0.81), indicative of a single copy of the transgenes in the parents. Furthermore, the parental, as well as pyramided progenies were evaluated for their potential resistance to aphids in terms of mortality and natality. The lines containing both the transgenes were found to be superior over single gene transgenics as a higher mortality rate (96%) was found in F2on the 9th day as compared to single gene transgenics (86% and 80% in P1 and P2 respectively). A significant decrease in the number of nymphs was observed in P1 and P2 but most in F2 plants as almost 43, 32.08, and 107.5 times decrease in the number of nymphs was found in P1, P2, and F2 individuals over control. Expression profiling was done to see if there was any impact of gene pyramiding on the expression pattern of both transgenes before and after aphid treatment, and no significant changes were observed, indicating constitutive expression of transgenes in pyramided lines also. In conclusion, pyramided lines were found to be promising and were superior for aphid resistance.