Determinación de CK-MB: interferencias en la utilización del método inmunológico / Determination of CK-MB: interferences in the use of an immunological method

En la práctica diaria, cuando se realiza la determinación de creatinquinasa isoenzima MB (CK-MB) por el método inmunológico, es habitual obtener resultados incongruentes: valores de CK-MB cercanos o superiores al de creatinquinasa total (CK). En el presente trabajo se informa acerca de las interferencias más frecuentes encontradas con este método en nuestro Instituto

Analysis of serum urinary proteins in normal and renal diseases by cellulose acetate electrophoresis.

A method using dried polyacrylamide gel to concentrate urine samples has been described, tested and used for the purpose of urine protein analysis. Concentrated urine samples from 10 normals and 100 patients with IgM nephropathy and systemic lupus erythematosus (SLE) were analysed by cellulose acetate electrophoresis (CAE). The results demonstrated that the patterns of proteins in the electrophoresis could be used to discriminate the two diseases. The best discriminating power was found in the logarithm of gamma globulin to albumin ratio. In IgM nephropathy the ratio of gamma globulin to albumin is much smaller than the ratio in SLE, indicating that relatively larger gamma globulins were excreted in SLE. In addition, the ratio can be used to discriminate subgroups of patients with IgM nephropathy. Urine from patients with IgM nephropathy with focal and segmental changes showed a significantly higher ratio. The study indicated the usefulness of the technique in discriminating the two common glomerular diseases.